In vitro assessment of decellularized porcine dermis as a matrix for urinary tract reconstruction

The contribution by the units in York and Leeds to tissue-engineering research is considerable, and they describe here their work to assess a natural matrix for use in the urinary tract, and to develop an in vitro regimen for assessing the biocompatibility of potential biomaterials. They describe how they have developed a simple, reproducible and rigorous regimen which will help to identify the causes of potential bio-incompatibility. Buccal mucosa has become the tissue of choice for urethroplasty. Authors from Edinburgh report on the oral complications after its harvesting; they found that most patients were satisfied with its use, but that it had long-term complications, about which patients should be informed. OBJECTIVES : To assess the potential of PermacolTM (Tissue Science Laboratories, Swillington, UK), a natural matrix derived from decellularized porcine dermis, as a matrix for urological tissue engineering, and thus to develop an in vitro regimen for assessing the biocompatibility of potential biomaterials before experimentation in animal models. MATERIALS AND METHODS : Urinary tract-derived normal human urothelial (NHU) and smooth muscle (SM) cells were grown in monoculture as autologous cell lines. Permacol was assessed for its ability to support colonization by NHU and SM cells. The failure of the Permacol matrix to be infiltrated by SM cells was further investigated using the highly invasive EJ bladder cancer cell line. RESULTS : NHU cells readily attached and grew as a monolayer on the surface of Permacol. Cells stratified when the culture medium was supplemented with 2 mmol/L calcium. EJ cells initially grew on the surface and subsequently invaded the matrix, while SM cells only colonized the surface of Permacol when cocultured with NHU cells. Cytoxicity, evaluated by contact inhibition and conditioned-medium assays, excluded the presence of soluble toxins in the biomaterial. CONCLUSIONS : We developed a simple, reproducible and rigorous regimen for assessing potential biomaterials in vitro. Applying this system might reduce the use of animals and help to identify causes of potential bio-incompatibility. The inability of SM cells to penetrate the Permacol matrix suggests that required matrix-bound signalling factors are absent, possibly as a result of the procedures used for processing Permacol. Identifying the key regulatory factors that regulate SM cell growth and orchestrate regenerative processes in the urinary tract will be important for developing suitable biomaterials for the bladder

Feasibility of noninvasive near-infrared spectroscopy to diagnose detrusor overactivity

Item does not contain fulltextIntroduction: Near-infrared spectroscopy (NIRS) is an optical technology able to detect the hemodynamic changes in biological tissues. Our objective was to determine the feasibility of applying NIRS in the noninvasive diagnosis of detrusor overactivity (DO). Patients and Methods: Comparative analysis was performed on 39 involuntary detrusor contractions (IDC) from 23 filling cystometries with simultaneous noninvasive NIRS of the bladder in 14 patients with DO. Motion artifacts were checked for via surface EMG of the abdominal muscles. Results: Thirty-nine IDC were obtained. The median amplitude of rise in detrusor pressure at DO was 48 cm H(2)O (range: 5-219). The median filling volume at DO was 148 ml (range: 9-531). NIRS curves demonstrated apparently significant deviations from baseline in 35 of 39 (90%) DO episodes. All onsets of NIRS deviations occurred within the time period of the IDC with a mean delay of 3 s (range: 0-9). Conclusion: NIRS can be a potential tool for the noninvasive diagnosis of DO