Improving cough and cold OTC medication use for children in Hardin County

Background: Ohio struggles with high infant mortality rates. This project was designed to combat incorrect use of cough and cold over-the-counter (OTC) medications. Parents or caregivers, especially those with low health literacy, often use these medications incorrectly in infants, sometimes leading to a fatal outcome. Methods: Utilizing a three-pronged approach, this IRB-approved project addresses the issue of incorrect cough and cold OTC medications use for infants in Hardin County. Passive education was provided to community members via social media/flyers, educational interventions were performed with community members (virtual or in-person), and continuing professional development (CPD) was provided to pharmacists and pharmacy technicians in Hardin County. Results: Passive education was disseminated in Hardin County. Thirty-eight community members attended the educational interventions; comparison of pre- and post-tests found statistically significant increases in knowledge (p=0.028). All participants indicated the information received was helpful, and 53% indicated they would change how cough and cold medications were administered. Thirteen professionals completed CPD; average post-test score=93%. Conclusion: It is important to teach parents and caregivers about proper use of cough and cold OTC medications. Education classes were effective in increasing community members’ knowledge, and CPD for pharmacy professionals reinforced opportunities for patient counseling

The Effect of Online Quizzing on Understanding of Key Concepts in an Introduction to Psychology Course

Students enrolled in Introduction to Psychology were required to complete online chapter-review quizzes as part of their course. Using a within-subjects experimental design, we compared the students’ understanding of key concepts during a week when they were required to take an online review quiz to their understanding of key concepts during a week when an online review quiz was not offered. Results suggest that online quizzing improves understanding of key chapter concepts

Gigantism in unique biogenic magnetite at the Paleocene-Eocene Thermal Maximum

We report the discovery of exceptionally large biogenic magnetite crystals in clay-rich sediments spanning the Paleocene-Eocene Thermal Maximum (PETM) in a borehole at Ancora, New Jersey. Aside from previously-described abundant bacterial magnetofossils, electron microscopy reveals novel spearhead-like and spindle-like magnetite up to 4 μm long and hexaoctahedral prisms up to 1.4 μm long. Similar to magnetite produced by magnetotactic bacteria, these single-crystal particles exhibit chemical composition, lattice perfection, and oxygen isotopes consistent with an aquatic origin. Electron holography indicates single-domain magnetization despite their large crystal size. We suggest that the development of a thick suboxic zone with high iron bioavailability – a product of dramatic changes in weathering and sedimentation patterns driven by severe global warming – drove diversification of magnetite-forming organisms, likely including eukaryotes

Activation of AMP-Activated Protein Kinase by Interleukin-6 in Rat Skeletal Muscle: Association With Changes in cAMP, Energy State, and Endogenous Fuel Mobilization

OBJECTIVE: Interleukin-6 (IL-6) directly activates AMP-activated protein kinase (AMPK) in vivo and in vitro; however, the mechanism by which it does so is unknown. RESEARCH DESIGN AND METHODS: We examined this question in skeletal muscle using an incubated rat extensor digitorum longus (EDL) muscle preparation as a tool. RESULTS: AMPK activation by IL-6 coincided temporally with a nearly threefold increase in the AMP:ATP ratio in the EDL. The effects of IL-6 on both AMPK activity and energy state were inhibited by coincubation with propranolol, suggesting involvement of β-adrenergic signaling. In keeping with this notion, IL-6 concurrently induced a transient increase in cAMP, and its ability to activate AMPK was blocked by the adenyl cyclase inhibitor 2′5′-dideoxyadenosine. In addition, like other β-adrenergic stimuli, IL-6 increased glycogen breakdown and lipolysis in the EDL. Similar effects of IL-6 on AMPK, energy state, and cAMP content were observed in C2C12 myotubes and gastrocnemius muscle in vivo, indicating that they were not unique to the incubated EDL. CONCLUSIONS: These studies demonstrate that IL-6 activates AMPK in skeletal muscle by increasing the concentration of cAMP and, secondarily, the AMP:ATP ratio. They also suggest that substantial increases in IL-6 concentrations, such as those that can result from its synthesis by muscles during exercise, may play a role in the mobilization of fuel stores within skeletal muscle as an added means of restoring energy balance.United States Public Health Service (RO1DK19514, RO1DK067509); Ruth L. Kirschstein NRSA Postdoctoral Training Grant (HL-07224); Fonds de la Recherche en Santé du Québe

Impact of animal strain on gene expression in a rat model of acute cardiac rejection

<p>Abstract</p> <p>Background</p> <p>The expression levels of many genes show wide natural variation among strains or populations. This study investigated the potential for animal strain-related genotypic differences to confound gene expression profiles in acute cellular rejection (ACR). Using a rat heart transplant model and 2 different rat strains (Dark Agouti, and Brown Norway), microarrays were performed on native hearts, transplanted hearts, and peripheral blood mononuclear cells (PBMC).</p> <p>Results</p> <p>In heart tissue, strain alone affected the expression of only 33 probesets while rejection affected the expression of 1368 probesets (FDR 10% and FC ≥ 3). Only 13 genes were affected by both strain and rejection, which was < 1% (13/1368) of all probesets differentially expressed in ACR. However, for PBMC, strain alone affected 265 probesets (FDR 10% and FC ≥ 3) and the addition of ACR had little further effect. Pathway analysis of these differentially expressed strain effect genes connected them with immune response, cell motility and cell death, functional themes that overlap with those related to ACR. After accounting for animal strain, additional analysis identified 30 PBMC candidate genes potentially associated with ACR.</p> <p>Conclusion</p> <p>In ACR, genetic background has a large impact on the transcriptome of immune cells, but not heart tissue. Gene expression studies of ACR should avoid study designs that require cross strain comparisons between leukocytes.</p

Search for Diffuse Neutral Hydrogen and HI Clouds in the NGC 2403 Group

We have observed the NGC 2403 group of galaxies using the Robert C. Byrd Green Bank Telescope (GBT) in a search for faint, extended neutral hydrogen clouds similar to the clouds found around the M81/M82 group, which is located approximately 250 kpc from the NGC 2403 group along the same filament of galaxies. For an HI cloud with a size < 10 kpc within 50 kpc of a group galaxy, our 7-sigma mass detection limit is 2.2 x 10^6 M_sun for a cloud with a linewidth of 20 km/s, over the velocity range from -890 to 1750 km/s. At this sensitivity level we detect 3 new HI clouds in the direction of the group, as well as the known galaxies. The mean velocity of the new clouds differs from that of the group galaxies by more than 250 km/s, but are in the range of Milky Way High Velocity Clouds (HVCs) in that direction. It is most likely that the clouds are part of the Milky Way HVC population. If HI clouds exist in the NGC 2403 group, their masses are less than 2.2 x 10^6 M_sun. We also compared our results to structures that are expected based on recent cosmological models, and found none of the predicted clouds. If NGC 2403 is surrounded by a population of dark matter halos similar to those proposed for the Milky Way in recent models, our observations imply that their HI content is less than 1% of their total mass.Comment: Accepted by A

Signal regularity-based automated seizure detection system for scalp EEG monitoring

Розглянуто роботу автоматизованої системи реєстрації ЕЕГ головного мозку для раннього виявлення епілептичних нападів. Розроблено комп’ютерний алгоритм для перетворення складних багатоканальних сигналів ЕЕГ мозку на кілька динамічних показників, супроводжуваних дослідженнями їхніх просторово-часових властивостей. Робота алгоритму аналізується на великому клінічному наборі даних.The purpose of the present study was to build a clinically useful automated seizure detection system for scalp EEG recordings. To achieve this, a computer algorithm was designed to translate complex multi-channel scalp EEG signals into several dynamical descriptors, followed by the investigations of their spatiotemporal properties that relate to the ictal (seizure) EEG patterns as well as to normal physiologic and artifact signals. This paper describes in detail this novel seizure detection algorithm and reports its performance in a large clinical dataset

Management of chemotherapy-associated febrile neutropenia

The development of febrile neutropenia during a course of chemotherapy is not only a life-threatening complication, it can also lead to a decision to reduce chemotherapy intensity in subsequent treatment cycles, thus putting patient outcomes at risk. Although there are strategies available for the primary prevention of febrile neutropenia, these are not widely used in the UK management of breast cancer. It is, therefore, paramount to have a well thought out and rigorously implemented care protocol for febrile neutropenia, involving patients, family/carers and health-care professionals in both primary and secondary care, to ensure early detection and effective management

Quantitative Proteomics of Intracellular Campylobacter jejuni Reveals Metabolic Reprogramming

Campylobacter jejuni is the major cause of bacterial food-borne illness in the USA and Europe. An important virulence attribute of this bacterial pathogen is its ability to enter and survive within host cells. Here we show through a quantitative proteomic analysis that upon entry into host cells, C. jejuni undergoes a significant metabolic downshift. Furthermore, our results indicate that intracellular C. jejuni reprograms its respiration, favoring the respiration of fumarate. These results explain the poor ability of C. jejuni obtained from infected cells to grow under standard laboratory conditions and provide the bases for the development of novel anti microbial strategies that would target relevant metabolic pathways

Satellite Cells Derived from Obese Humans with Type 2 Diabetes and Differentiated into Myocytes In Vitro Exhibit Abnormal Response to IL-6

Obesity and type 2 diabetes are associated with chronically elevated systemic levels of IL-6, a pro-inflammatory cytokine with a role in skeletal muscle metabolism that signals through the IL-6 receptor (IL-6Rα). We hypothesized that skeletal muscle in obesity-associated type 2 diabetes develops a resistance to IL-6. By utilizing western blot analysis, we demonstrate that IL-6Rα protein was down regulated in skeletal muscle biopsies from obese persons with and without type 2 diabetes. To further investigate the status of IL-6 signaling in skeletal muscle in obesity-associated type 2 diabetes, we isolated satellite cells from skeletal muscle of people that were healthy (He), obese (Ob) or were obese and had type 2 diabetes (DM), and differentiated them in vitro into myocytes. Down-regulation of IL-6Rα was conserved in Ob myocytes. In addition, acute IL-6 administration for 30, 60 and 120 minutes, resulted in a down-regulation of IL-6Rα protein in Ob myocytes compared to both He myocytes (P<0.05) and DM myocytes (P<0.05). Interestingly, there was a strong time-dependent regulation of IL-6Rα protein in response to IL-6 (P<0.001) in He myocytes, not present in the other groups. Assessing downstream signaling, DM, but not Ob myocytes demonstrated a trend towards an increased protein phosphorylation of STAT3 in DM myocytes (P = 0.067) accompanied by a reduced SOCS3 protein induction (P<0.05), in response to IL-6 administration. Despite this loss of negative control, IL-6 failed to increase AMPKα2 activity and IL-6 mRNA expression in DM myocytes. There was no difference in fusion capacity of myocytes between cell groups. Our data suggest that negative control of IL-6 signaling is increased in myocytes in obesity, whereas a dysfunctional IL-6 signaling is established further downstream of IL-6Rα in DM myocytes, possibly representing a novel mechanism by which skeletal muscle function is compromised in type 2 diabetes