Culture media supplemented with inorganic salts improve the growth and viability of several bacterial strains

In order to improve growth and storage conditions for bacterial cultures, commonly used basic culture media, Luria-Bertani broth (LB) and glucose-yeast extract (GY) were tested along with their supplemented versions (LBS and GYS) containing a complex set of inorganic salts required for common physiological processes. The growth kinetics and viability of 15 representative strains were compared on LB/LBS or GY/GYS. Growth kinetics were examined during a 24 h period. Five out of 15 strains showed enhanced growth on LBS and GYS. Three strains showed very low viability (3 months or lower) both on the basic and salt-supplemented media. Six strains could be equally recovered after 6 or 12 months both from LB/LBS and from GY/GYS. Six of the tested 15 bacterial strains showed significantly better recovery rate on the inorganic-supplemented media LBS or GYS than on basic LB or GY. These results show that inorganic supplement of basic media may significantly improve the growth and viability of several bacterial strains

The introduction and spreading of the fitness-wellness service from the beginning to the present days

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Tevékenységszervezést támogató vizuális segítségek tervezése és kivitelezése

INST: L_408A „DATA” projekt egy új, magyar nyelven is elérhető, tevékenység szervezést támogató applikáció kidolgozására irányul. A mintatartalmak kidolgozásáért az ELTE keretein belül egy – Havasi Ágnes és Bertók Csilla vezetésével működő – team felelős, melynek az egyetemen tanuló diákok is részesei lehetnek. Hallgatóként, az utóbbi félévben csatlakoztam ehhez a csapathoz. Szakdolgozatom keretében a kormányablakban történő ügyintézéshez és néhány, ehhez kapcsolódó online ügyintézési folyamathoz terveztem és készítettem vizuális segítségeket

Culture media supplemented with inorganic salts improve the growth and viability of several bacterial strains

In order to improve growth and storage conditions for bacterial cultures, commonly used basic culture media, Luria-Bertani broth (LB) and glucose-yeast extract (GY) were tested along with their supplemented versions (LBS and GYS) containing a complex set of inorganic salts required for common physiological processes. The growth kinetics and viability of 15 representative strains were compared on LB/LBS or GY/GYS. Growth kinetics were examined during a 24 h period. Five out of 15 strains showed enhanced growth on LBS and GYS. Three strains showed very low viability (3 months or lower) both on the basic and salt-supplemented media. Six strains could be equally recovered after 6 or 12 months both from LB/LBS and from GY/GYS. Six of the tested 15 bacterial strains showed significantly better recovery rate on the inorganic-supplemented media LBS or GYS than on basic LB or GY. These results show that inorganic supplement of basic media may significantly improve the growth and viability of several bacterial strains

Immortelle (<i>Helichrysum italicum</i> (Roth) G. Don) Essential Oil Showed Antibacterial and Biofilm Inhibitory Activity against Respiratory Tract Pathogens

The biofilm formation of bacteria in different parts of the human body can influence the success of antibiotic therapy. Essential oils (EOs) and their components are becoming increasingly popular in point of view of medicinal applications, because of their antibacterial efficacy. The immortelle EO has been used traditionally as an expectorant; however, there are no studies summarizing its antibacterial effect against respiratory tract bacteria. Our aim was to investigate the antibacterial and biofilm inhibitory activity of immortelle (Helichrysum italicum) EO against respiratory tract pathogens such as Haemophilus influenzae, H. parainfluenzae, Pseudomonas aeruginosa and Streptococcus pneumoniae. In order to prove the antibacterial effect of the immortelle EO, broth microdilution and biofilm inhibition tests, and membrane damage assay were investigated. Scanning electron microscopy was used to identify the structural modifications in bacterial cells. Our results showed that immortelle EO has antibacterial and anti-biofilm effects against respiratory tract bacteria used in this study. H. parainfluenzae was the most sensitive to each treatment, however, P. aeruginosa was the most resistant bacteria. In conclusion, the studied EO may have a role in the treatment of respiratory tract infections due to their antibacterial and anti-biofilm activity