Mapping the physiological states of Carnobacterium maltaromaticum obtained” by applying stressful fermentation conditions

This work received funding from the European Union´s Horizon 2020 research and innovation programme under grant agreement No 777657Mapping the physiological states of Carnobacterium maltaromaticum obtained” by applying stressful fermentation conditions. 4. Microbial Stress: from systems to molecules and bac

Application of Lactic Acid Bacteria in Time-Temperature Integrators

International audienceBiological Time-Temperature Integrators (TTI) offer a novel approach for improving food safety and preventing spoilage. These smart tools relay, by an irreversible color shift, the cumulative effect of time and temperature on the microbial quality of food they are attached to. Among various types of TTI developed to date, biological TTI have the advantage of reproducing microbiological spoilage reactions that take place in food. They are based on the pH decline of a medium contained within the label, resulting from lactic acid bacteria (LAB) growth and acidification. When developing LAB-based TTI, careful LAB strain selection, research, and development efforts on TTI production are necessary to closely match the behavior of both spoilage and pathogenic microorganisms that grow during the storage of perishable foods. Covering a wide range of time-temperature profiles is a challenging goal involving research in different domains (microbiology, food science, modelling, etc.). This chapter describes the design and working principle of LAB-based TTI, how they are parametrized to be able to track a wide range shelf-lives and how their performance is evaluated. Current applications and future prospects of this innovative way of using lactic acid bacteria are also discussed

Reproductive performance of common dentex, Dentex dentex, broodstock held under different photoperiod and constant temperature conditions

International audienc

Reproductive performance of common dentex, Dentex dentex, broodstock held under different photoperiod and constant temperature conditions

International audienc

Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization.

International audiencePseudomonas aeruginosa plays a major role in cystic fibrosis (CF) progression. Therefore, it is important to understand the initial steps of P. aeruginosa infection. The structure and dynamics of CF respiratory tract microbial communities during the early stages of P. aeruginosa colonization were characterized by pyrosequencing and cloning-sequencing. The respiratory microbiota showed high diversity, related to the young age of the CF cohort (mean age 10 years). Wide inter- and intra-individual variations were revealed. A common core microbiota of 5 phyla and 13 predominant genera was found, the majority of which were obligate anaerobes. A few genera were significantly more prevalent in patients never infected by P. aeruginosa. Persistence of an anaerobic core microbiota regardless of P. aeruginosa status suggests a major role of certain anaerobes in the pathophysiology of lung infections in CF. Some genera may be potential biomarkers of pulmonary infection state

<em>Porphyromonas</em>, a potential predictive biomarker of Pseudomonas aeruginosa pulmonary infection in cystic fibrosis

Additional material is published online only. To view please visit the journal online (http://dx.doi.org/10.1136/bmjresp-2018-000374).International audienceIntroduction : Pseudomonas aeruginosa pulmonary infections are the primary cause of morbi-mortality in patients with cystic fibrosis (CF). In this cohort study, the objective was to identify candidate biomarkers of P. aeruginosa infection within the airway microbiota. Methods : A 3-year prospective multicentre study (PYOMUCO study) was conducted in Western France and included patients initially P. aeruginosa free for at least 1year. A 16S-targeted metagenomics approach was applied on iterative sputum samples of a first set of patients (n=33). The composition of airway microbiota was compared according to their P. aeruginosa status at the end of the follow-up (colonised vs non-colonised), and biomarkers associated with P. aeruginosa were screened. In a second step, the distribution of a candidate biomarker according to the two groups of patients was verified by qPCR on a second set of patients (n=52) coming from the same cohort and its load quantified throughout the follow-up. Results : Porphyromonas (mainly P. catoniae) was found to be an enriched phylotype in patients uninfected by P. aeruginosa (pDiscussion : Further studies on replication cohorts are needed to validate this potential predictive biomarker, which may be relevant for the follow-up in the early years of patients with CF. The identification of infection candidate biomarkers may offer new strategies for CF prevision medicine

Preserving bacteria with oligosaccharides and eco-friendly processes (Premium)

Funding H2020 - MSCA RISE PREMIUM n° 777567Preserving bacteria with oligosaccharides and eco-friendly processes (Premium). 55. Annual Meeting of the Society for Cryobiolog