Axillary node metastasis from differentiated thyroid carcinoma with hürthle and signet ring cell differentiation. A case of disseminated thyroid cancer with peculiar histologic findings

<p>Abstract</p> <p>Background</p> <p>Differentiated thyroid cancer is usually associated with an excellent prognosis and indolent course. Distant metastases are rare events at the onset of thyroid cancer. Among these presentations, metastasis to the axillary lymph nodes is even more unusual: only few cases were previously reported in the literature; there has been no report of axillary lymph node metastasis from follicular thyroid carcinoma. Axillary lymph node metastasis generally arises in the context of disseminated disease and carries an ominous prognosis.</p> <p>Case presentation</p> <p>Here we present a case of axillary lymph node metastasis in the context of disseminated differentiated thyroid cancer. The patient underwent near total thyroidectomy and neck and axillary lymph node dissection. A histopathological diagnosis of poorly differentiated follicular carcinoma with "signet ring cells" and Hürthle cell features was established. The patient received radioactive iodine therapy and TSH suppression therapy. Subsequently his serum thyroglobulin level decreased to 44.000 ng/ml from over 100.000 ng/ml.</p> <p>Discussion and Conclusion</p> <p>Currently there are only few reported cases of axillary node metastases from thyroid cancer, and to our knowledge, this is the first report on axillary lymph node metastasis from follicular thyroid carcinoma. "Signet ring cell" is a morphologic feature shared by both benign and, more rarely, malignant follicular thyroid neoplasm, and it generally correlates with an arrest in folliculogenesis. Our case is one of the rare "signet ring cells" carcinomas so far described.</p

Extracts of some indigenous plants affecting hatching and mortality in the root-knot nematode [Meloidogyne javanica (Treub) Chitwood]

The root-knot nematodes (Meloidogyne spp.) (RKNs) are one of the major pests of vegetables which cause yield losses due to galling and reduction in root development and shoot growth. Herein, effects of five different plants extracts; pepper, Capsicum frutescents (Cf); henbane, Hyoscyamus niger (Hn); bead-tree, Melia azedarah (Ma); common cocklebur, Xanthium strumarium (Xs); and yarrow, Achillea wilhelmsii (Aw) on eggs and second stage juveniles (J2s) of the RKN (Meloidogyne javanica) were evaluated in vitro tests. All plant extracts showed different levels of anti-nematode’s activity. Plant extract concentrations of 3, 6 and 12% for Hn, Xs and Ma caused 100% inhibition of egg hatching, followed by Cf and Aw. Ma and Xs were more effective for inducing mortality among J2s than the other tested extracts. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved

Extracts of Some Indigenous Plants Affecting Hatching and Mortality in the Root-Knot Nematode [Meloidogyne javanica (Treub) Chitwood]

WOS: 000358899300006The root-knot nematodes (Meloidogyne spp.) (RKNs) are one of the major pests of vegetables which cause yield losses due to galling and reduction in root development and shoot growth. Herein, effects of five different plants extracts; pepper, Capsicum frutescents (Cf); henbane, Hyoscyamus niger (bin); bead-tree, Melia azedarah (Ma); common cocklebur, Xanthium strumarium (Xs); and yarrow, Achillea wilhelmsii (Aw) on eggs and second stage juveniles (J2s) of the RKN (Meloidogyne javanica) were evaluated in vitro tests. All plant extracts showed different levels of anti-nematode's activity. Plant extract concentrations of 3, 6 and 12% for Hn, Xs and Ma caused 100% inhibition of egg hatching, followed by Cf and Aw. Ma and Xs were more effective for inducing mortality among J2s than the other tested extracts.Scientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK 111O784]The authors are grateful to Assoc. Prof. Dr. Z. Devran (Dept. of Plant Protection, Fac. of Agriculture, Antalya Univ., Antalya, Turkey) for supplying the root-knot nematode (M. javanica). Thanks also to the Plant Protection Central Research Institute, Ankara, Turkey for supplying the plant extracts. This study was supported by the Scientific and Technical Research Council of Turkey (TUBITAK 111O784)

Effects of Some Indigenous Plant Extracts on Mortality of the Root Lesion Nematode, Pratylenchus thornei Sher & Allen

WOS: 000373756700021The genus of root lesion nematodes (RLN), Pratylenchus, feeds and reproduces in the root cortex of many plant species. RLN was reproduced on carrot culture by using a pure culture. In vitro tests were carried out to determine the effects of plant extracts from three indigenous plants: henbane, Hyoscyamus niger; bead-tree, Melia azedarah and common cocklebur, Xanthium strumarium on P. thornei (the major wheat root-lesion nematode). The nematodes were exposed for 12, 24, 48, 72 and 96 hrs in three concentrations of plant extracts. The effect of plant extracts was performed using the suspensions of P. thornei (100 nematodes ml(-1)) in distilled water. Distilled water was used as a control. All dishes were kept at 28 +/- 2 degrees C. The plant extracts of H. niger, M. azedarah and X strumarium exhibited highly promising mortality rate (100%) after 72 hours of exposure time. The result of this study suggests the studied plant extracts to be used for reducing the P. thornei population

Effects of some indigenous plant extracts on mortality of the root lesion nematode, Pratylenchus thornei Sher & Allen

The genus of root lesion nematodes (RLN), Pratylenchus, feeds and reproduces in the root cortex of many plant species. RLN was reproduced on carrot culture by using a pure culture. In vitro tests were carried out to determine the effects of plant extracts from three indigenous plants: henbane, Hyoscyamus niger; bead-tree, Melia azedarah and common cocklebur, Xanthium strumarium on P. thornei (the major wheat root-lesion nematode). The nematodes were exposed for 12, 24, 48, 72 and 96 hrs in three concentrations of plant extracts. The effect of plant extracts was performed using the suspensions of P. thornei (100 nematodes ml-1) in distilled water. Distilled water was used as a control. All dishes were kept at 28±2°C. The plant extracts of H. niger, M. azedarah and X. strumarium exhibited highly promising mortality rate (100%) after 72 hours of exposure time. The result of this study suggests the studied plant extracts to be used for reducing the P. thornei population. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved

Identification of Turkish isolate of the entomopathogenic fungi, Purpureocillium lilacinum (syn: Paecilomyces lilacinus) and its effect on potato pests, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) and Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

Potato (Solanum tuberosum L.) is one of the most important vegetable crops in Turkey as well in the world. Potato plants are attacked by a large number of insect pests such as aphids, beetles, leafhoppers and lepidopterous pests under both field and storage conditions. The Colorado potato beetle (CPB) [Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)] and the potato tuber moth (PTM) [Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)] are the most destructive pests in potatoes grown areas. Many pesticides that used to control the potato pests are very toxic to environment and human. An alternative approach to the commercial pesticides is using biological agents like fungi. Purpureocillium lilacinum was described more than a century ago and is a commonly occurring fungus in soil. P. lilacinum (syn: Paecilomyces lilacinus) is the most widely tested fungus for the control of nematodes, aphids and insects. The fungus was determined in Turkey but its molecular identification has not been done yet. In this study, a Turkish isolate of P. lilacinum was identified by classical (morphologic and morphometric properties) and molecular methods. Also, the study concerned with the effect of different conidial concentrations of the fungal isolate on mortality rates of CPB and PTM at different temperatures. P. lilacinumwas found to be most effective on the last larval instar of CPB and PTM (33.2 and 43.3% mortality, respectively) on 10th day of treatment with the fungal concentration of 108cfu ml-1at 25°C. This fungus developed on all dead larvae and was highly susceptible at the tested temperatures, but its activity decreased at 15°C. Findings of this study showed a potential of the fungus as a bioagent against the CPB and the PTM. © 2016, Egyptian Society for Biological Control of Pests. All rights reserved

Nematicidal Activity of Beauveria bassiana (Bals.-Criv.) Vuill. against Root-Knot Nematodes on Tomato Grown under Natural Conditions

WOS: 000400123900016Plant parasitic nematodes such as root-knot nematodes (RKNs) are a major threat to vegetables, fruits and ornamental plants which cause yield loss. One of the best alternative methods of nematode control is using the entomopathogenic fungi (EPF). The aim of this study was to evaluate the effects of two Turkish isolates of entomopathogenic fungi, Beauveria bassiana (Bals.-Criv.) Vuill. (F-56 and F-63), against naturally infected mixed populations of Meloidogyne incognita and Meloidogyne javanica on tomatoes under natural conditions. The experiment contained 3 conidial suspensions (10(6), 10(7) and 10(8) cfu ml(-1)) with 4 replicates of each treatment. A total of 32 plants were arranged in a randomized block. EPF were applied 4 times (15 days before planting, planting, 15, and 30 days after planting). Nematicide [Paecilomyces lilacinus strain 251 (BioAct (R))] was used as a positive control. At the end of the growing season, the efficiency of the applications was determined according to the root gall index (I) and yields of tomatoes (II). The fungi concentration of 10(8) cfu ml(-1) was significantly greater than in the others. This concentration from both isolates [F-56 (4.59 +/- 0.54) and F-63 (2.77 +/- 0.59)] controlled M. incognita and M. javanica and increased the yield of tomatoes. The results demonstrated the suitability of using B. bassiana isolate F-56 and F-63 for reducing RKNs on tomatoes under greenhouse conditions

Identification of Turkish Isolate of the Entomopathogenic Fungi, Purpureocillium lilacinum (syn: Paecilomyces lilacinus) and its Effect on Potato Pests, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae) and Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

WOS: 000358899300019Potato (Solanum tuberosum L.) is one of the most important vegetable crops in Turkey as well in the world. Potato plants are attacked by a large number of insect pests such as aphids, beetles, leafhoppers and lepidopterous pests under both field and storage conditions. The Colorado potato beetle (CPB) [Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)] and the potato tuber moth (PTM) [Phihorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)] are the most destructive pests in potatoes grown areas. Many pesticides that used to control the potato pests are very toxic to environment and human. An alternative approach to the commercial pesticides is using biological agents like fungi. Purpureocillium lilacinum was described more than a century ago and is a commonly occurring fungus in soil. P. lilacinum (syn: Paecilomyces lilacinus) is the most widely tested fungus for the control of nematodes, aphids and insects. The fungus was determined in Turkey but its molecular identification has not been done yet. In this study, a Turkish isolate of P. lilacinum was identified by classical (morphologic and morphometric properties) and molecular methods. Also, the study concerned with the effect of different conidial concentrations of the fungal isolate on mortality rates of CPB and PTM at different temperatures. P. lilacinum was found to be most effective on the last larval instar of CPB and PTM (33.2 and 43.3% mortality, respectively) on 10th day of treatment with the fungal concentration of 108cfu ml(-1) at 25 degrees C. This fungus developed on all dead larvae and was highly susceptible at the tested temperatures, but its activity decreased at 15 degrees C. Findings of this study showed a potential of the fungus as a bioagent against the CPB and the PTM.Scientific and Technical Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TUBITAK 111O784]A part of this study (preparation of P. lilacinum) was supported by the Scientific and Technical Research Council of Turkey (TUBITAK 111O784). Thanks are due to Drs. F.D. Erdogus and M. Alkan (Dept. of Entomology, Plant Protection Central Research Institute, Ankara-Turkey) for their assisstance throughout this study

A new culturing method for the rice white tip nematode, Aphelenchoides besseyi Christie, 1942, on carrot discs

[No abstract available