Analisis Mutasi Gen Protein X Virus Hbv Pada Penderita Hepatitis B Akut Di Manado

Faktor-faktor yang mempengaruhi perkembangan hepatitis B kronis menjadi kanker hati antara lain mutasi pada gen x. Penelitian ini bertujuan untuk mengidentifikasi gen protein x virus HBV dan menganalisis apakah terjadi mutasi gen yang terkait dengan munculnya tumor ganas sirosis hati (HCC). Penelitian ini menggunakan primer untuk proses nested PCR yang telah dirancang sebelumnya. Proses nested PCR terhadap 10 sampel DNA HBV pasien dilakukan untuk mengamplifikasi fragmen DNA gen x dilanjutkan dengan sekuensing untuk penentuan urutan nukleotida gen x. Urutan nukleotida gen x diterjemahkan menjadi urutan asam amino protein x. Terhadap hasil penentuan urutan asam amino protein x, dianalisis apakah terdapat keberadaan mutasi dikaitkan dengan hepatitis B akut. Identifikasi molekuler menunjukkan bahwa dari 10 sampel DNA HBV pasien yang uji, 5 sampel yang berhasil ditentukan urutan nukleotida gen xnya. Hasil analisis mutasi gen, ditemukan adanya mutasi gen protein x, tapi tidak ada satupun ditemukan adanya mutasi yang terkait dengan HCC

Prevalensi Hiperurisemia Pada Remaja Obes Di Smk Negeri 1 Bitung

Uric acid is an end product of purine metabolism of substances derived from the rest of the food we eat. Substances contained in any materials purine foods we eat both animals and plants. Some studies show an association between uric acid with hypertension, obesity kidney disease and cardiovasculer disease. The associated factors with the encidance of hyperuricemia is sex, BMI, carbohydrate intake, and intake of purines. This study aimed to determine the prevalence of obesity among adolescents in SMK Negeri 1 Bitung and prevalence of hyperuricemia in obese adolescents at SMK Negeri 1 Bitung. The method was used an observational cross-sectional approach. Data obtained by measuring waist circumference and laboratory examinations are taking blood specimens for examination uric acid levels. From the 100 students representing adolescents were screened by measuring waist circumference, which includes criteria obese students is 24 students (24 %) and non-obese students (76 %). A total of 10 students who met the criteria were examined obese uric acid levels, there was 1 female students (1%) who had hyperuricemia. As control of the research conducted checks on 10 non-obese students to examine the levels of uric acid, there were 6 students (6%) who hyperuricemia consisting of 2 boys (2%) and 4 female students (4%). The prevalence of obese adolescents in biutng quite high (24%) and the incidane of hyperuricemia 10 students who meet the criteria and are willing to be obese subjects is 1 students (1%) and the incidance of hyperuricemia of 10 students who are willing to serve non-obese subjects were 6 students (6%). It was concluded that tehere wass no association the obesity with hyperuricemia

Kajian Anti Piretik Dan Anti Oksidan Dari Ekstrak Alga Hijau Boergesenia Forbesii

Alga laut yang tumbuh secara liar di pinggiran perairan merupakan salah satu tumbuhan laut yang berpotensi penting untuk industri farmasitika Indonesia. Alga hijau Boergesenia forbesii merupakan salah satu yang alga liar yang memiliki potensi untuk dikembangkan. Dalam penelitian ini akan diuji aktifitas ekstrak alga hijau Boergesenia forbesii apakah memiliki aktifitas antipiretik. Metode yang digunakan untuk uji antipiretik yaitu menaikkan suhu tubuh dari hewan uji dengan memberikan larutan pepton 10% dengan dosis 150 mg/kg. Kontrol positif menggunakan 1% parasitamol dengan dosis 150 mg/kg. Hewan perlakuan menggunakan ekstrak alga hijau Boergesenia forbesii 1% denga dosis 150 mg/kg. Hasil yang diperoleh menunjukkan ekstrak alga hijau Boergesenia forbesii memiliki aktifitas antipiretik. Tetapi perlu dilakukan penelitan lanjutan untuk diperoleh senyawa yang lebih murni lagi

Hubungan Antara Status Gizi Dengan Kadar Gula Darah Puasa Pada Masyarakat Kelurahan Bahu Kecamatan Malalayang Manado

: Nutrition Status is state of the body that is the end result of a balance between the nutrients into the body and its use. One way to monitor nutrition status of adults is by measuring body mass index. Blood glucose levels be used as a parameter to the success of the metabolism in the body. In a certain state with respect to glucose levels in the body can occur hypoglycemia or hiperglikemia. The purpose of research this is to know the relation of nutrition status with fasting blood glucose levels in people in the village of Bahu. Method: this type of research is obeservasional analytic with cross sectional design. The number of samples as much as 63 people taken by simple random sampling in bahu village. Data obtained process used by chi squre test with degrees of significance (α) = 0,05. The result showed that nutrition status with normal body mass index there was 8 person who has fasting blood glucose levels <110mg/dl and 3 person has fasting blood glucose levels ≥110 mg/dl. While nutrition status with obese body mass index there was 35 person who has fasting blood glucose levels <110mg/dl and 17 person has fasting blood glucose levels ≥110 mg/dl. Conclusion in this research was not relationship between nutrition status with fasting blood glucose levels with value p=1,0

Hubungan Obesitas Dengan Tekanan Darah Dan Aktivitas Fisik Pada Remaja Di Kota Bitung

: Obesity is one of the global health problem which is often found. Indonesia is a developing country with high incident of obesity. Adolescents are the age group with high levels of obesity risk. The increasing of obesity followed by increasing co-morbidity the potential to be disease in a later time. Obesity increasing the risk of high blood pressure. Hypertension in adolescents already many found. Hypertension in adolescents is problem because it can be continued in adults. one important factor which play a role in obesity is physical activity. Objective: This research to determine the relationship of obesity with blood pressure and physical activity in adolescents in Bitung. Methodology: This study was observational analytic cross sectional design. Results: There were 60 subjekts as the subject of study that meets the inclusions kriteria which have been measured. Consisted of 21 boys and 39 girls. Of 60 obese adolescents are 34 adolescents with high blood pressure, 26 adolescent with normal blood pressure, 52 adolescent with moderate physical activity, 8 adolescents with low physical activity, there is no a significant relationship with blood pressure (Chi Square Test p=0,120), and there is no a significant relationship with physical activity (Chi Square Test: p=0,486. Conclusion: There is no a relationship between obesity with blood pressure and physical activity in adolescents in Bitung