Updated Constraints and Future Prospects on Majoron Dark Matter

Majorons are (pseudo-)Nambu-Goldstone bosons associated with lepton number symmetry breaking due to the Majorana mass term of neutrinos introduced in the seesaw mechanism. They are good dark matter candidates since their lifetime is suppressed by the lepton number breaking scale. We update constraints and discuss future prospects on majoron dark matter in the singlet majoron models based on neutrino, gamma-ray, and cosmic-ray telescopes in the mass region of MeV--10 TeV.Comment: 25 pages, 6 figures, 3 table

Constraints on dark matter-neutrino scattering from the Milky-Way satellites and subhalo modeling for dark acoustic oscillations

The elastic scattering between dark matter (DM) and radiation can potentially explain small-scale observations that the cold dark matter faces as a challenge, as damping density fluctuations via dark acoustic oscillations in the early universe erases small-scale structure. We study a semi-analytical subhalo model for interacting dark matter with radiation, based on the extended Press-Schechter formalism and subhalos' tidal evolution prescription. We also test the elastic scattering between DM and neutrinos using observations of Milky-Way satellites from the Dark Energy Survey and PanSTARRS1. We conservatively impose strong constraints on the DM-neutrino scattering cross section of $\sigma_{{\rm DM}\text{-}\nu,n}\propto E_\nu^n$ $(n=0,2,4)$ at $95\%$ confidence level (CL), $\sigma_{{\rm DM}\text{-}\nu,0}< 10^{-32}\ {\rm cm^2}\ (m_{\rm DM}/{\rm GeV})$, $\sigma_{{\rm DM}\text{-}\nu,2}< 10^{-43}\ {\rm cm^2}\ (m_{\rm DM}/{\rm GeV})(E_\nu/E_{\nu}^0)^2$ and $\sigma_{{\rm DM}\text{-}\nu,4}< 10^{-54}\ {\rm cm^2}\ (m_{\rm DM}/{\rm GeV})(E_\nu/E_{\nu}^0)^4$, where $E_\nu$ is the neutrino energy and $E_\nu^0$ is the average momentum of relic cosmic neutrinos today, $E_\nu^0 \simeq 6.1\ {\rm K}$. By imposing a satellite forming condition, we obtain the strongest upper bounds on the DM-neutrino cross section at$95\%$CL,$\sigma_{{\rm DM}\text{-}\nu,0}< 4\times 10^{-34}\ {\rm cm^2}\ (m_{\rm DM}/{\rm GeV})$,$\sigma_{{\rm DM}\text{-}\nu,2}< 10^{-46}\ {\rm cm^2}\ (m_{\rm DM}/{\rm GeV})(E_\nu/E_{\nu}^0)^2$and$\sigma_{{\rm DM}\text{-}\nu,4}< 7\times 10^{-59}\ {\rm cm^2}\ (m_{\rm DM}/{\rm GeV})(E_\nu/E_{\nu}^0)^4$.Comment: 19 pages, 7 figures. v2: Matches the published version in JCA

Neutrino lines from MeV dark matter annihilation and decay in JUNO

We discuss the discovery potential of JUNO experiment for neutrino lines from MeV dark matter (DM) annihilation and decay in a model independent way. We find that JUNO will be able to give severe constraints on the cross section of DM annihilating into neutrinos and on the lifetime of DM decaying into neutrinos. More concretely, with $20$ years of data-taking in the fiducial volume $17$ kton, the cross section will be constrained smaller than $4\times 10^{-26}\,{\rm cm^{3}\,sec^{-1}}$ for the mass of a DM particle $15\,{\rm MeV} \lesssim m_{\chi} \lesssim 50\,{\rm MeV}$ at $90\,\%$ C.L., which might be strong enough to test thermal production mechanism of DM particles for such range of DM mass. The lifetime will be constrained as strong as $1\times 10^{24}\,{\rm sec}$ for the mass of a DM particle $m_{\chi} \simeq 100\,{\rm MeV}$ at $90\,\%$ C.L..Comment: v2: 26 pages,10 figures, added profile dependence, subdominant neutrino interactions, and references as well as made some correction

Arterial spin labeling image findings in the acute phase in paediatric patients with acute encephalopathy with biphasic seizures and late reduced diffusion

IntroductionDiagnosing acute encephalopathy with biphasic seizures and late reduced diffusion (AESD) after the first seizure (early seizure/seizures, ES/ESs) is challenging because a reduced apparent diffusion coefficient (ADC) in the cortical or subcortical white matter, often described as having a “bright-tree appearance (BTA),” is usually not observed until secondary seizures (late seizures, LSs) occur. Previous studies have reported hypoperfusion on arterial spin labeling (ASL) within 24 h after ES/ESs in patients with AESD and hyperperfusion within 24 h after LS onset. This study aimed to investigate cerebral blood flow in the hyperacute phase (between ES/ESs and LSs) using ASL in patients with AESD.MethodsEight ASL images were acquired in six patients with AESD admitted to our hospital from October 2021 to October 2022. ASL findings in the hyperacute phase were investigated and video-electroencephalogram findings obtained around ASL image acquisition in the hyperacute phase were evaluated.ResultsFour ASL images were obtained for three patients before LS onset, with three images showing hyperperfusion areas and one image showing hypoperfusion areas. These hyperperfuion regions coincided with BTA on subsequent images of these patients.In one patient, the first ASL image was obtained in the late hyperacute phase and revealed hyperperfusion areas with a slightly abnormal change on diffusion-weighted image (DWI), which were not accompanied by ADC abnormalities. The second ASL image obtained 51 h after the first ASL, and before LS onset revealed more prominent hyperperfusion areas than the first ASL image, which were accompanied by BTA. In another patient, the ASL image obtained 82 h after ES revealed hyperperfusion areas without abnormal change on DWI or ADC.ConclusionThis study revealed that two patients exhibited hyperperfusion regions and another patient exhibited hypoperfusion regions among three patients who underwent ASL imaging during the period from 24 h after ES/ESs to LSs in patients with LSs or cooling initiation in patients without LSs due to early anaesthesia induction (late hyperacute phase). Further prospective studies on cerebral blood flow are required to explore the relationship among the timing of image acquisition, the presence of electrographic seizures, and ASL findings in patients with AESD

An oxyl/oxo mechanism for dioxygen bond formation in PSII revealed by X-ray free electron lasers

Photosynthetic water oxidation is catalyzed by the Mn4CaO5 cluster of photosystem II (PSII) with linear progression through five S-state intermediates (S0 to S4). To reveal the mechanism of water oxidation, we analyzed structures of PSII in the S1, S2, and S3 states by x-ray free-electron laser serial crystallography. No insertion of water was found in S2, but flipping of D1 Glu189 upon transition to S3 leads to the opening of a water channel and provides a space for incorporation of an additional oxygen ligand, resulting in an open cubane Mn4CaO6 cluster with an oxyl/oxo bridge. Structural changes of PSII between the different S states reveal cooperative action of substrate water access, proton release, and dioxygen formation in photosynthetic water oxidation

Capturing structural changes of the S-1 to S-2 transition of photosystem II using time-resolved serial femtosecond crystallography

Photosystem II (PSII) catalyzes light-induced water oxidation through an S-i-state cycle, leading to the generation of di-oxygen, protons and electrons. Pumpprobe time-resolved serial femtosecond crystallography (TR-SFX) has been used to capture structural dynamics of light-sensitive proteins. In this approach, it is crucial to avoid light contamination in the samples when analyzing a particular reaction intermediate. Here, a method for determining a condition that avoids light contamination of the PSII microcrystals while minimizing sample consumption in TR-SFX is described. By swapping the pump and probe pulses with a very short delay between them, the structural changes that occur during the S-1-to-S-2 transition were examined and a boundary of the excitation region was accurately determined. With the sample flow rate and concomitant illumination conditions determined, the S-2-state structure of PSII could be analyzed at room temperature, revealing the structural changes that occur during the S-1-to-S-2 transition at ambient temperature. Though the structure of the manganese cluster was similar to previous studies, the behaviors of the water molecules in the two channels (O1 and O4 channels) were found to be different. By comparing with the previous studies performed at low temperature or with a different delay time, the possible channels for water inlet and structural changes important for the water-splitting reaction were revealed

Selective depletion of mouse kidney proximal straight tubule cells causes acute kidney injury

The proximal straight tubule (S3 segment) of the kidney is highly susceptible to ischemia and toxic insults but has a remarkable capacity to repair its structure and function. In response to such injuries, complex processes take place to regenerate the epithelial cells of the S3 segment; however, the precise molecular mechanisms of this regeneration are still being investigated. By applying the “toxin receptor mediated cell knockout” method under the control of the S3 segment-specific promoter/enhancer, Gsl5, which drives core 2 β-1,6-N-acetylglucosaminyltransferase gene expression, we established a transgenic mouse line expressing the human diphtheria toxin (DT) receptor only in the S3 segment. The administration of DT to these transgenic mice caused the selective ablation of S3 segment cells in a dose-dependent manner, and transgenic mice exhibited polyuria containing serum albumin and subsequently developed oliguria. An increase in the concentration of blood urea nitrogen was also observed, and the peak BUN levels occurred 3–7 days after DT administration. Histological analysis revealed that the most severe injury occurred in the S3 segments of the proximal tubule, in which tubular cells were exfoliated into the tubular lumen. In addition, aquaporin 7, which is localized exclusively to the S3 segment, was diminished. These results indicate that this transgenic mouse can suffer acute kidney injury (AKI) caused by S3 segment-specific damage after DT administration. This transgenic line offers an excellent model to uncover the mechanisms of AKI and its rapid recovery

Oxygen-evolving photosystem II structures during S1–S2–S3 transitions

Photosystem II (PSII) catalyses the oxidation of water through a four-step cycle of Si states (i = 0–4) at the Mn4CaO5 cluster1,2,3, during which an extra oxygen (O6) is incorporated at the S3 state to form a possible dioxygen4,5,6,7. Structural changes of the metal cluster and its environment during the S-state transitions have been studied on the microsecond timescale. Here we use pump-probe serial femtosecond crystallography to reveal the structural dynamics of PSII from nanoseconds to milliseconds after illumination with one flash (1F) or two flashes (2F). YZ, a tyrosine residue that connects the reaction centre P680 and the Mn4CaO5 cluster, showed structural changes on a nanosecond timescale, as did its surrounding amino acid residues and water molecules, reflecting the fast transfer of electrons and protons after flash illumination. Notably, one water molecule emerged in the vicinity of Glu189 of the D1 subunit of PSII (D1-E189), and was bound to the Ca2+ ion on a sub-microsecond timescale after 2F illumination. This water molecule disappeared later with the concomitant increase of O6, suggesting that it is the origin of O6. We also observed concerted movements of water molecules in the O1, O4 and Cl-1 channels and their surrounding amino acid residues to complete the sequence of electron transfer, proton release and substrate water delivery. These results provide crucial insights into the structural dynamics of PSII during S-state transitions as well as O–O bond formation