20 research outputs found

    HC.auto.2pops

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    This file is a text file in Arelquin input format. It could easily be manipulated into other formats. The 2 groups represent autosome data for 2 species C. urophasianus and C. minimus

    Z.3pops.4.arlequin

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    This is a text file in Arlequin input format and represents Z chromosome data for three groups (2 C. urophasianus - one (GRSG) is the southern part of the range and the other (BiState) is the diverged population on the border between California and Nevada). The third group is Gunnison Sage-grouse

    HC.auto.3pops

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    This file is a text file in Arelquin input format. It contains autosome SNP genotypes for three groups. Two groups of C. urophasianus (one is GRSG which represents the southern part of the range and the other is BiState which represents the diverged population on the border between California and Nevada). The third group is the Gunnison Sage-grouse data

    mean FST by chromosome

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    This file is a text file that shows the mean FST values between C. urophasianus and C. minimus for each chromosome (1 - 10) as well as the Z chromosome

    Additional file 1: Table S1 of Unexpected effects of different genetic backgrounds on identification of genomic rearrangements via whole-genome next generation sequencing

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    The numbers of CTXs in each sample after filtering process and chromosome coordinates of all CTXs. 10 sequenced samples include 6 tumor samples (119J, 125J, 196J, 202J, 46J, and 90J) and 4 control samples (mouse control 1, mouse control 2, kidney and wt B6) plus 129S1 whose sequences were downloaded from Sanger’s Institute (see details in Methods). (XLSX 541 kb

    Preliminary investigation of a hypertonic saline nasal rinse as a hygienic intervention in dairy workers

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    Livestock workers experience an increased burden of bioaerosol-induced respiratory disease including a high prevalence of rhinosinusitis. Dairy operations generate bioaerosols spanning the inhalable size fraction (0–100 μm) containing bacterial constituents such as endotoxin. Particles with an aerodynamic diameter between 10 and 100 μm are known to deposit in the nasopharyngeal region and likely affect the upper respiratory tract. We evaluated the effectiveness of a hypertonic saline nasal lavage in reducing inflammatory responses in dairy workers from a high-volume dairy operation. Inhalable personal breathing zone samples and pre-/post-shift nasal lavage samples from each participant over five consecutive days were collected. The treatment group (n = 5) received hypertonic saline while the control group (n = 5) received normotonic saline. Personal breathing zone samples were analyzed for particulate concentrations and endotoxin using gravimetric and enzymatic methods, respectively. Pro- and anti-inflammatory cytokines (i.e., IL-8, IL-10, and TNF-α) were measured from nasal lavage samples using a multiplex assay. Inhalable dust concentrations ranged from 0.15 to 1.9 mg/m3. Concentrations of both pro- and anti-inflammatory cytokines, specifically IL-6, IL-8, and IL-10, were significantly higher in the treatment group compared to the control group (p p p < 0.01, respectively). Further analysis of IL-10 anti-inflammatory indicates a positive association between hypertonic saline administration and IL-10 production. This pilot study demonstrates that hypertonic saline nasal lavages were successful in upregulating anti-inflammatory cytokines to support larger interventional studies.</p

    Additional file 2: Figure S1. of Unexpected effects of different genetic backgrounds on identification of genomic rearrangements via whole-genome next generation sequencing

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    Numbers of Candidate SV Calls Before Filtering Process. The numbers of candidate SV calls detected in 10 samples of different genetic backgrounds before any filtering process. The numbers of total SVs include ITX (intra-chromosomal translocations), DELs (deletions), INV (inversions), INS (insertions), and CTXs (inter-chromosomal translocations) in 10 sequenced samples, including 6 tumor samples (119J, 125J, 196J, 202J, 46J, and 90J) and 4 control samples (control 1, control 2, kidney and wt B6) plus 129S1 whose sequences were downloaded from Sanger’s Institute (see details in Methods). (PDF 361 kb
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