Analysis of Ribosomal Protein Gene Structures: Implications for Intron Evolution

Many spliceosomal introns exist in the eukaryotic nuclear genome. Despite much research, the evolution of spliceosomal introns remains poorly understood. In this paper, we tried to gain insights into intron evolution from a novel perspective by comparing the gene structures of cytoplasmic ribosomal proteins (CRPs) and mitochondrial ribosomal proteins (MRPs), which are held to be of archaeal and bacterial origin, respectively. We analyzed 25 homologous pairs of CRP and MRP genes that together had a total of 527 intron positions. We found that all 12 of the intron positions shared by CRP and MRP genes resulted from parallel intron gains and none could be considered to be “conserved,” i.e., descendants of the same ancestor. This was supported further by the high frequency of proto-splice sites at these shared positions; proto-splice sites are proposed to be sites for intron insertion. Although we could not definitively disprove that spliceosomal introns were already present in the last universal common ancestor, our results lend more support to the idea that introns were gained late. At least, our results show that MRP genes were intronless at the time of endosymbiosis. The parallel intron gains between CRP and MRP genes accounted for 2.3% of total intron positions, which should provide a reliable estimate for future inferences of intron evolution

Mesobiliverdin IXα Enhances Rat Pancreatic Islet Yield and Function

The aims of this study were to produce mesobiliverdin IXα, an analog of anti-inflammatory biliverdin IXα, and to test its ability to enhance rat pancreatic islet yield for allograft transplantation into diabetic recipients. Mesobiliverdin IXα was synthesized from phycocyanobilin derived from cyanobacteria, and its identity and purity were analyzed by chromatographic and spectroscopic methods. Mesobiliverdin IXα was a substrate for human NADPH biliverdin reductase. Excised Lewis rat pancreata infused with mesobiliverdin IXα and biliverdin IXα-HCl (1–100 μM) yielded islet equivalents as high as 86.7 and 36.5%, respectively, above those from non-treated controls, and the islets showed a high degree of viability based on dithizone staining. When transplanted into livers of streptozotocin-induced diabetic rats, islets from pancreata infused with mesobiliverdin IXα lowered non-fasting blood glucose (BG) levels in 55.6% of the recipients and in 22.2% of control recipients. In intravenous glucose tolerance tests, fasting BG levels of 56 post-operative day recipients with islets from mesobiliverdin IXα infused pancreata were lower than those for controls and showed responses that indicate recovery of insulin-dependent function. In conclusion, mesobiliverdin IXα infusion of pancreata enhanced yields of functional islets capable of reversing insulin dysfunction in diabetic recipients. Since its production is scalable, mesobiliverdin IXα has clinical potential as a protectant of pancreatic islets for allograft transplantation

Characteristics of electron internal transport barrier in Heliotron J

The formation of an electron internal transport barrier (eITB) has been observed for the first time with centrally focused electron cyclotron heating (ECH) microwaves injected into plasma in Heliotron J. When the heating power per electron density (${P}_{\mathrm{ECH}}/{\bar{n}}_{{\rm{e}}}$) exceeds a threshold of $250\times {10}^{-19}\,\mathrm{kW}\,{{\rm{m}}}^{3}$, transient increases of both the central Te and the core Te gradients are observed. A neoclassical (NC) calculation using the Sugama–Nishimura moment method predicts that the large positive radial electric field (Er) is formed in the core region. Heat transport analysis shows a significant reduction of the effective electron thermal diffusivity in the plasma with the eITB related to that without the eITB. The large gap between the experimentally obtained effective thermal diffusivity and the NC thermal diffusivity suggests that the suppression of anomalous transport contributes to the core improved confinement of the eITB plasma. The electron cyclotron emission measurement shows both the transient increase and the hysteresis phenomena during the eITB formation

Predicting the outcome of chronic kidney disease by the estimated nephron number: The rationale and design of PRONEP, a prospective, multicenter, observational cohort study

<p>Abstract</p> <p>Background</p> <p>The nephron number is thought to be associated with the outcome of chronic kidney disease (CKD). If the nephron number can be estimated in the clinical setting, it could become a strong tool to predict renal outcome. This study was designed to estimate the nephron number in CKD patients and to establish a method to predict the outcome by using the estimated nephron number.</p> <p>Methods/Design</p> <p>The hypothesis of this study is that the estimated nephron number can predict the outcome of a CKD patient. This will be a multicenter, prospective (minimum 3 and maximum 5 years follow-up) study. The subjects will comprise CKD patients aged over 14 years who have undergone a kidney biopsy. From January 2011 to March 2013, we will recruit 600 CKD patients from 10 hospitals belonging to the National Hospital Organization of Japan. The primary parameter for assessment is the composite of total mortality, renal death, cerebro-cardiovascular events, and a 50% reduction in the eGFR. The secondary parameter is the rate of eGFR decline per year. The nephron number will be estimated by the glomerular density in biopsy specimens and the renal cortex volume. This study includes one sub-cohort study to establish the equation to calculate the renal cortex volume. Enrollment will be performed at the time of the kidney biopsy, and the data will consist of a medical interview, ultrasound for measurement of the kidney size, blood or urine test, and the pathological findings of the kidney biopsy. Patients will continue to have medical consultations and receive examinations and/or treatment as usual. The data from the patients will be collected once a year after the kidney biopsy until March 2016. All data using this study are easily obtained in routine clinical practice.</p> <p>Discussion</p> <p>This study includes the first trials to estimate the renal cortex volume and nephron number in the general clinical setting. Furthermore, this is the first prospective study to examine whether the nephron number predicts the outcome of CKD patients. The results from this study should provide powerful new tools for nephrologists in routine clinical practice.</p> <p>Trial registration</p> <p>UMIN-Clinical Trial Registration, UMIN000004784.</p

Collective Thomson scattering diagnostic with in situ calibration system for velocity space analysis in large helical device

A collective Thomson scattering (CTS) diagnostic with a ±3 GHz band around a 77 GHz gyrotron probe beam was developed to measure the velocity distribution of bulk and fast ions in high-temperature plasmas. We propose a new in situ calibration method for a CTS diagnostic system combined with a raytracing code. The method is applied in two situations for electron cyclotron emission in plasmas and in a CTS diagnostic with a modulated probe beam. Experimental results highlight the importance of refraction correction in probe and receive beams. The CTS spectrum is measured with the in situ calibrated CTS receiver and responds to fast ions originating from a tangential neutral beam with an energy of 170 keV and from a perpendicular beam with an energy of 60 keV, both in the large helical device. From a velocity space analysis model, the results elucidate the measured anisotropic CTS spectrum caused by fast ions. The calibration methods and analyses demonstrated here are essential for CTS, millimeter-wave diagnostics, and electron cyclotron heating required under fusion reactor conditions

Calibrations of the LHD Thomson scattering system

The Thomson scattering diagnostic systems are widely used for the measurements of absolute local electron temperatures and densities of fusion plasmas. In order to obtain accurate and reliable temperature and density data, careful calibrations of the system are required. We have tried several calibration methods since the second LHD experiment campaign in 1998. We summarize the current status of the calibration methods for the electron temperature and density measurements by the LHD Thomson scattering diagnostic system. Future plans are briefly discussed

Response of a core coherent density oscillation on electron cyclotron resonance heating in Heliotron J plasma

We report properties of a coherent density oscillation observed in the core region and its response to electron cyclotron resonance heating (ECH) in Heliotron J plasma. The measurement was performed using a multi-channel beam emission spectroscopy system. The density oscillation is observed in a radial region between the core and the half radius. The poloidal mode number is found to be 1 (or 2). By modulating the ECH power with 100 Hz, repetition of formation and deformation of a strong electron temperature gradient, which is likely ascribed to be an electron internal transport barrier, is realized. Amplitude and rotation frequency of the coherent density oscillation sitting at the strong electron temperature gradient location are modulated by the ECH, while the poloidal mode structure remains almost unchanged. The change in the rotation velocity in the laboratory frame is derived. Assuming that the change of the rotation velocity is given by the background E × B velocity, a possible time evolution of the radial electric field was deduced

Collective Thomson scattering with 77, 154, and 300 GHz sources in LHD

Collective Thomson scattering (CTS) is one of attractive diagnostics for measuring locally and directly the fuel temperature and the velocity distribution of fast ions in fusion plasmas. A mega-watt class source of millimeter or sub-millimeter waves is required to detect a weak scattered radiation superimposed on background radiation owing to electron cyclotron emissions (ECEs) from plasmas. Based on electron cyclotron resonance heating (ECRH) system with the frequencies of 77 GHz and 154 GHz in the Large Helical Device (LHD), the CTS diagnostic system has been developed to measure bulk ion temperatures from a few keV to ∼10 keV and fast ions originated from 180 keV-neutral beam injection in the LHD. The measured CTS spectra and their time evolutions are analyzed with the electrostatic scattering theory. The bulk ion temperatures obtained from CTS spectra increase with the neutral beam injections and decrease with the heating terminated. The velocity map of simulated fast ions explains that the bumps on tail of measured CTS spectra are caused by the co- and counter- fast ions. A new prescription for anisotropic velocity distribution function is proposed. As for 154 GHz bands, the CTS spectrum broadenings for D and H plasmas are distinguished reasonably at the same temperature, and its ion temperatures are comparable to those of the charge exchange recombination spectroscopy. As reactor-relevant diagnostics, a 300 GHz gyrotron and a corresponding receiver system have been implemented in LHD to access high density plasmas with low background ECEs. The recent progress for CTS diagnostics and their spectrum analysis with the probe frequencies of 77 GHz, 154 GHz, and 300 GHz in the LHD experiments is described

First World Consensus Conference on pancreas transplantation: Part II - recommendations.

Funder: Fondazione Pisa, Pisa, Italy; Id: http://dx.doi.org/10.13039/100007368Funder: Tuscany Region, Italy; Id: http://dx.doi.org/10.13039/501100009888Funder: Pisa University Hospital, Pisa, ItalyFunder: University of Pisa, Pisa, Italy; Id: http://dx.doi.org/10.13039/501100007514The First World Consensus Conference on Pancreas Transplantation provided 49 jury deliberations regarding the impact of pancreas transplantation on the treatment of diabetic patients, and 110 experts' recommendations for the practice of pancreas transplantation. The main message from this consensus conference is that both simultaneous pancreas-kidney transplantation (SPK) and pancreas transplantation alone can improve long-term patient survival, and all types of pancreas transplantation dramatically improve the quality of life of recipients. Pancreas transplantation may also improve the course of chronic complications of diabetes, depending on their severity. Therefore, the advantages of pancreas transplantation appear to clearly surpass potential disadvantages. Pancreas after kidney transplantation increases the risk of mortality only in the early period after transplantation, but is associated with improved life expectancy thereafter. Additionally, preemptive SPK, when compared to SPK performed in patients undergoing dialysis, appears to be associated with improved outcomes. Time on dialysis has negative prognostic implications in SPK recipients. Increased long-term survival, improvement in the course of diabetic complications, and amelioration of quality of life justify preferential allocation of kidney grafts to SPK recipients. Audience discussions and live voting are available online at the following URL address: http://mediaeventi.unipi.it/category/1st-world-consensus-conference-of-pancreas-transplantation/246