Arjunolic acid derivative glycoside from the stems of Hedera colchica

Five triterpenoid saponins were isolated from the stems of Hedera colchica K. Koch, Araliaceae. Two of them are new natural substances. HCSt-A (1): 3-O-α-D-arabinopyranoside; 28-O-α-L-rhamnopyranosyl-(1→4)-O- β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl-arjunolic acid. HCSt-B (2): 3-O-β-D-xylopyranoside; 28-O-α-L-rhamnopyranosyl- (1→4)-O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl- hederagenin. The derivative of arjunolic acid is described for the first time in Araliaceae family. The chemical structures of isolated compounds were established on the base of chemical and 1D and 2D NMR experiments

Arjunolic acid derivative glycoside from the stems of Hedera colchica

Five triterpenoid saponins were isolated from the stems of Hedera colchica K. Koch (Araliaceae). Two of them are new natural substances. HCS-A (1): 3-O-α-L-arabinopyranoside, 28-O-α-L-rhamnopyranosyl-(1→ 4)-O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl-arjunolic acid. HCSt-B (2):3-O-β-D-xylopyranoside, 28-O-α-L-rhamnopyranosyl- (1→4)-O-β-D-glucopyranosyl-(1→6)-O-β-D-glucopyranosyl- hederagenin. A derivative of arjunolic acid is described for the first time in the Araliaceae family. The chemical structures of isolated compounds were established on the basis of chemical and 1D and 2D NMR experiments

Antifungal and antiprotozoal activities of saponins from Hedera colchica

Of the seven triterpene glycosides present in the leaves of Hedera colchica C. Koch, (ram. Araliaceae) - Colchis ivy, which we have called hederacolchisides, we have previously isolated and characterized the three most polar [i, 2]. In continuation of these investigations, we have succeeded in isolating another three, comparatively nonpolar, compounds present in the total in minor amount - hederacolchisides A', A, and C

Isolation of a new disaccharide nucleoside from Helleborus caucasicus : structure elucidation and total synthesis of hellecaucaside A and its β-anomer

Hellecaucaside A, a new disaccharide nucleoside featuring a 2'-O-alpha-D-ribofuranosyluridine skeleton and a 4-hydroxybenzoyl group at the 50 position, was isolated from the underground part of Helleborus caucasicus. The structure of the compound was elucidated by means of chemical degradation and spectroscopic analyses, such as 1D/2D NMR, chiral-GC, and HRMS. The total synthesis of hellecaucaside A and its beta-anomer was accomplished, unequivocally confirming the structure of the natural product

In vitro antileishmanial activity of three saponins isolated from ivy, α-hederin, β-hederin and hederacolchiside A1, in association with pentamidine and amphotericin B

The in vitro antileishmanial activity of three saponins isolated from ivy, α-hederin, β-hederin and hederacolchiside A1, was investigated on parasites of the species Leishmania mexicana, in their promastigote and amastigote forms compared with their toxicity versus human monocytes. The results showed that saponins exhibited a strong antiproliferative activity on all stages of development of the parasite but demonstrated a strong toxicity versus human cells. Association of subtoxic concentrations of saponins with antileishmanial drugs such as pentamidine and amphotericin B demonstrated that saponins could enhance the efficiency of conventional drugs on both the promastigote and the amastigote stages of development of the parasite. The results demonstrated moreover that the action of saponins on promastigote membrane was cumulative with those of amphotericin B

Antileishmanial activity of three saponins isolated from ivy, α- hederin, β-hederin and hederacolchiside A1, as compared to their action on mammalian cells cultured in vitro

The in vitro antileishmanial activity of three saponins isolated from ivy, α-hederin, β-hederin and hederacolchiside A1, was investigated on Leishmania infantum. The assessment of possible targets (membrane integrity, membrane potential, DNA synthesis and protein content) was performed in both Leishmania promastigotes and human monocytes (THP1 cells). Results observed in Leishmania showed that the saponins exhibited a strong antiproliferative activity on all stages of development of the parasite by altering membrane integrity and potential: hederacolchiside A1 appeared to be the most active compound against both promastigotes and amastigotes. Results observed in THP1 cells demonstrated that the saponins exerted also a potent antiproliferative activity against human monocytes, by producing a significant DNA synthesis inhibition. The ratio between antileishmanial activity on amastigotes and toxicity to human cells suggested that the saponins could be considered as possible antileishmanial drugs