Role of potassium channel Kir4.1(KCNJ10) in the wound healing of human corneal epithelial cells

Thesis (M.A.)--Boston UniversityThe cornea, which is key to maintaining our normal vision by refracting light onto the lens and retina, as well as serving as a physical barrier to protect our eyes from the environment, contains epithelium tissue with one of the highest capacities for renewal and wound healing in the body. Although many studies have looked at the process of wound healing in corneal epithelium, most of them have focused on the various ligand-receptor growth factor signaling pathways, and few studies have been done to study the signaling and regulations of wound healing that are initiated intracellularly, or those associated with electrical currents and channel activity. This study, therefore, aims to look at the hypotheses that injury to the corneal epithelium leads to the downregulation of a type of potassium transport channels named Kir4.1/KCNJ10, and that the inhibition of KCNJ10 is associated with intracellular microRNA-205 (miR-205), which is upregulated during injury and healing. Together, the modulations in the level of KCNJ10 and microRNA-205 contribute to change in the environment around the wound and promote the cellular processes that allow for efficient corneal healing. To investigate the role of potassium channels and microRNAs in the cornea epithelium, an in-vitro model of endogenous wound healing was employed with human corneal epithelium cells (HCECs) serving as the primary model of study. Physiological injury was simulated using a scratch-wound model. The protein expressions for KCNJ10 and microRNA-205 were measured through various time points from both control and injured HCECs. The effect of two RNAi modulators of microRNA-205, a mimic and antagonist, and of KCNJ10 blockers were also tested for effects on the rate and efficiency of HCEC wound healing. Results indicated that the expression of miR-205 increased in scratch-injured HCECs and that the expression of KCNJ10 decreased in wounded and healing HCECs. It was also shown that increasing KCNJ10 and decreasing miR-205 both lead to delayed healing, but that blocking KCNJ10 could partially abolished the effect of delayed healing associated with decreasing miR-205 and restored the healing process. It was also shown that the 3'UTR of KCNJ10 contains potential target sites for miR-205 binding and action. The results indicate that that KCNJ10 expression is negatively associated with corneal wound healing, and that miR-205 is upregulated upon injury in wounded corneal epithelium to inhibit KCNJ10 and allow for the processes of wound healing to take place

Merging history of massive galaxies at 3<z<6

The observational data of high redshift galaxies become increasingly abundant, especially since the operation of the James Webb Space Telescope (JWST), which allows us to verify and optimize the galaxy formation model at high redshifts. In this work, we investigate the merging history of massive galaxies at $3 < z < 6$ using a well-developed semi-analytic galaxy formation catalogue. We find that the major merger rate increases with redshift up to 3 and then flattens. The fraction of wet mergers, during which the sum of the cold gas mass is higher than the sum of the stellar mass in two merging galaxies, also increases from $\sim$ 34\% at $z = 0$ to 96\% at $z = 3$. Interestingly, almost all major mergers are wet at $z > 3$ . This can be attributed to the high fraction ($> 50\%$) of cold gas at $z > 3$. In addition, we study some special systems of massive merging galaxies at $3 < z < 6$, including the massive gas-rich major merging systems and extreme dense proto-clusters, and investigate the supermassive black hole-dark matter halo mass relation and dual AGNs. We find that the galaxy formation model reproduces the incidence of those observed massive galaxies, but fails to reproduce the relation between the supermassive black hole mass and the dark matter halo mass at $z \sim 6$. The latter requires more careful estimates of the supermassive black hole masses observationally. Otherwise, it could suggest modifications of the modeling of the supermassive black hole growth at high redshifts.Comment: 9 pages,8figure

Cross-subject EEG-based emotion recognition through dynamic optimization of random forest with sparrow search algorithm

The objective of EEG-based emotion recognition is to classify emotions by decoding signals, with potential applications in the fields of artificial intelligence and bioinformatics. Cross-subject emotion recognition is more difficult than intra-subject emotion recognition. The poor adaptability of classification model parameters is a significant factor of low accuracy in cross-subject emotion recognition. We propose a model of a dynamically optimized Random Forest based on the Sparrow Search Algorithm (SSA-RF). The decision trees number (DTN) and the leave minimum number (LMN) of the RF are dynamically optimized by the SSA. 12 features are used to construct feature combinations for selecting the optimal feature combination. DEAP and SEED datasets are employed for testing the performance of SSA-RF. The experimental results show that the accuracy of binary classification is 76.81% on DEAP, and the accuracy of triple classification is 75.96% on SEED based on SSA-RF, which are both higher than that of traditional RF. This study provides new insights for the development of cross-subject emotion recognition, and has significant theoretical value

Comparison of the effects of rumen-protected and unprotected L-leucine on fermentation parameters, bacterial composition, and amino acids metabolism in in vitro rumen batch cultures

This study was conducted to compare the effects of rumen-protected (RP-Leu) and unprotected L-leucine (RU-Leu) on the fermentation parameters, bacterial composition, and amino acid metabolism in vitro rumen batch incubation. The 5.00 g RP-Leu or RU-Leu products were incubated in situ in the rumen of four beef cattle (Bos taurus) and removed after 0, 2, 4, 6, 12, 16, and 24 h to determine the rumen protection rate. In in vitro incubation, both RP-Leu and RU-Leu were supplemented 1.5 mmol/bottle (L-leucine HCl), and incubated after 0, 2, 4, 6, 8, 12, and 16 h to measure gas production (GP), nutrient degradability, fermentation parameters, bacterial composition, and amino acids metabolism. Results from both in vitro and in situ experiments confirmed that the rumen protection rate was greater (p &lt; 0.01) in RP-Leu than in RU-Leu, whereas the latter was slow (p &lt; 0.05) degraded within incubation 8 h. Free leucine from RP-Leu and RU-Leu reached a peak at incubation 6 h (p &lt; 0.01). RU-Leu supplementation increased (p &lt; 0.05) gas production, microbial crude protein, branched-chain AAs, propionate and branched-chain VFAs concentrations, and Shannon and Sobs index in comparison to the control and RP-Leu supplementation. RU-Leu and RP-Leu supplementation decreased (p &lt; 0.05) the relative abundance of Bacteroidota, which Firmicutes increased (p &lt; 0.05). Correlation analysis indicated that there are 5 bacteria at the genus level that may be positively correlated with MCP and propionate (p &lt; 0.05). Based on the result, we found that RP-Leu was more stable than RU-Leu in rumen fluid, but RU-Leu also does not exhibit rapid degradation by ruminal microbes for a short time. The RU-Leu was more beneficial in terms of regulating rumen fermentation pattern, microbial crude protein synthesis, and branched-chain VFAs production than RP-Leu in vitro rumen conditions

Age- and time-of-day dependence of glymphatic function in the human brain measured via two diffusion MRI methods

Advanced age, accompanied by impaired glymphatic function, is a key risk factor for many neurodegenerative diseases. To study age-related differences in the human glymphatic system, we measured the influx and efflux activities of the glymphatic system via two non-invasive diffusion magnetic resonance imaging (MRI) methods, ultra-long echo time and low-b diffusion tensor imaging (DTIlow–b) measuring the subarachnoid space (SAS) flow along the middle cerebral artery and DTI analysis along the perivascular space (DTI-ALPS) along medullary veins in 22 healthy volunteers (aged 21–75 years). We first evaluated the circadian rhythm dependence of the glymphatic activity by repeating the MRI measurements at five time points from 8:00 to 23:00 and found no time-of-day dependence in the awake state under the current sensitivity of MRI measurements. Further test–retest analysis demonstrated high repeatability of both diffusion MRI measurements, suggesting their reliability. Additionally, the influx rate of the glymphatic system was significantly higher in participants aged &gt;45 years than in participants aged 21–38, while the efflux rate was significantly lower in those aged &gt;45 years. The mismatched influx and efflux activities in the glymphatic system might be due to age-related changes in arterial pulsation and aquaporin-4 polarization

Simulation and Experimental Study on Reverse Helical Milling with the Gradual-Removal Reverse Edge Milling Cutter under Ultrasonic-Assisted Condition

As a new machining method, ultrasonic-assisted bi-direction helical milling has obvious advantages in making holes on carbon fiber-reinforced plastics (CFRP). However, cutting edges of the flat-bottomed milling cutter are easy to wear, which may cause severe defects such as burrs and tears in the outlet of the hole. In order to improve the hole-making quality of CFRP, the gradual-removal reverse edge milling cutter was proposed and designed. The finite method models of reverse helical milling CFRP with the flat-bottomed reverse edge milling cutter and the gradual-removal reverse edge milling cutter under an ultrasonic vibration were established, and the comparative cutting experiments of the two cutters were carried out. By comparing the cutting performance of the two milling cutters under the condition of ultrasonic vibration assistance, the cutting mechanism of improving the hole wall quality by the gradual-removal reverse edge milling cutter was studied. The results showed that when the reverse cumulative cutting depth reached about 60 mm, compared with the flat-bottomed reverse edge milling cutter, the gradual-removal reverse edge milling cutter transferred part of the cutting task of the peripheral edge to the end edge, and the wear of the reverse peripheral edges which directly affects the hole quality was effectively alleviated. This mechanism made the cutting state of the peripheral edge dominated by shear failure, which led to the significant improvement of the quality at the outlet of the hole

A Study of Eye-Tracking Gaze Point Classification and Application Based on Conditional Random Field

The head-mounted eye-tracking technology is often used to manipulate the motion of servo platform in remote tasks, so as to achieve visual aiming of servo platform, which is a highly integrated human-computer interaction effect. However, it is difficult to achieve accurate manipulation for the uncertain meanings of gaze points in eye-tracking. To solve this problem, a method of classifying gaze points based on a conditional random field is proposed. It first describes the features of gaze points and gaze images, according to the eye visual characteristic. An LSTM model is then introduced to merge these two features. Afterwards, the merge features are learned by CRF model to obtain the classified gaze points. Finally, the meaning of gaze point is classified for target, in order to accurately manipulate the servo platform. The experimental results show that the proposed method can classify more accurate target gaze points for 100 images, the average evaluation values Precision = 86.81%, Recall = 86.79%, We = 86.79%, these are better than relevant methods. In addition, the isolated gaze points can be eliminated, and the meanings of gaze points can be classified to achieve the accuracy of servo platform visual aiming

CD19: a biomarker for B cell development, lymphoma diagnosis and therapy

<p>Abstract</p> <p>The human CD19 antigen is a 95 kd transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD19 is classified as a type I transmembrane protein, with a single transmembrane domain, a cytoplasmic C-terminus, and extracellular N-terminus. CD19 is a biomarker for normal and neoplastic B cells, as well as follicular dendritic cells. CD19 is critically involved in establishing intrinsic B cell signaling thresholds through modulating both B cell receptor-dependent and independent signaling. CD19 functions as the dominant signaling component of a multimolecular complex on the surface of mature B cells, alongside complement receptor CD21, and the tetraspanin membrane protein CD81 (TAPA-1), as well as CD225. Through study of CD19 transgenic and knockout mouse models, it becomes clear that CD19 plays a critical role in maintaining the balance between humoral, antigen-induced response and tolerance induction. This review also summarized latest clinical development of CD19 antibodies, anti-B4-bR (an immunotoxin conjugate), blinatumomab (BiTE), and SAR3419 (huB4-DM4), a novel antibody-drug conjugate.</p

Privacy Protection Scheme for the Internet of Vehicles Based on Private Set Intersection

Performing location-based services in a secure and efficient manner that remains a huge challenge for the Internet of Vehicles with numerous privacy and security risks. However, most of the existing privacy protection schemes are based on centralized location servers, which makes them all have a common drawback of a single point of failure and leaking user privacy. The employment of anonymity and cryptography is a well-known solution to the above problem, but its expensive resource consumption and complex cryptographic operations are difficult problems to solve. Based on this, designing a distributed and privacy-secure privacy protection scheme for the Internet of Vehicles is an urgent issue for the smart city. In this paper, we propose a privacy protection scheme for the Internet of Vehicles based on privacy set intersection. Specially, using privacy set intersection and blockchain techniques, we propose two protocols, that is, a dual authentication protocol and a service recommendation protocol. The double authentication protocol not only ensures that both communicating parties are trusted users, but also ensures the reliability of their session keys; while the service recommendation protocol based on pseudorandom function and one-way hash function can well protect the location privacy of users from being leaked. Finally, we theoretically analyze the security that this scheme has, i.e., privacy security, non-repudiation, and anti-man-in-the-middle attack