Investigation of SRP9 protein expression in breast cancer

Background Signal recognition particle (SRP) promotes co-translational translocation of the proteins through or into the endoplasmic reticulum membrane and it also has elongation arrest function. SRP9 is one of the six protein subunits of SRP and functions in elongation arrest activity by forming a heterodimeric structure with SRP14. It is one of the substrates of ADAR, which has been found to have a role in breast cancer. This study was conducted to investigate the SRP9 protein expression in normal and tumor tissues of patients with breast cancer and determine its prognostic significance. Methods and results A total of 32 female patients who were diagnosed as having primary breast cancer and underwent surgery were included in the study. Western Blotting was performed to detect SRP9 protein expression levels in normal and tumor tissue samples. Clinical and pathologic characteristics were analyzed to assess the prognostic significance. SRP9 protein expression was statistically higher in the breast cancer tissue samples compared to normal matched tissue, and the mean SRP9 protein expression levels of breast cancer tissue normal tissue samples were 1.019 +/- 1.011 and 0.551 +/- 0.456, respectively (p = 0.001). SRP9 protein expression levels in tumor tissue of patients with lymph node metastasis, tumor size > 2 cm, estrogen receptor-positive, progesterone receptor-positive, and HER-2 negative were statistically higher than in normal tissue (p < 0.05). Conclusions It is vital to clarify the roles of molecules such as SRP9 in understanding the pathogenesis of breast cancer. In our study, we showed that SRP9 expression increased in breast cancer and was associated with disease-related parameters

Pathology and Biobanking

Biobanks are units where high quality and long-term protection of biomaterials is maintained. This system, in which biological materials and data are systematically recorded and stored, is a unique resource for the study of the pathophysiology of disease, the development of diagnostic biomarkers, and working with human tissues for the potential discovery of targeted therapeutic agents. At this point, the pathology unit plays a unifying and complementary role between the clinical and core disciplines and offers optimal management of the patients' biomaterials for diagnostic and research projects. The aim of this article is to present general information with regard to a biobank constructed for the storage of tumor tissue and blood biospecimens

MOLEKÜLER PATOLOJİ LABORATUVAR YÖNTEMLERİ VE BİYOİNFORMATİK YAKLAŞIM

Günümüzde moleküler incelemelerin gerçekleştirildiği moleküler patoloji laboratuvar sayısı gün geçtikçe artmaktadır. Moleküler yöntemlerle doğru vekaliteli sonuç elde edebilmek moleküler patoloji laboratuvar yapılanmasının uygun olmasından geçmektedir. Bir moleküler patoloji laboratuvarı kurmak için yeterli alan, uygun ekipman ve deneyimlipersonel gerekmektedir. Ayrıca kontaminasyondankaçınmak için gerekliliklerin yerine getirilmesi veolası kontaminasyon durumlarında uygulanması gereken işlemlerin de bilinmesi çok önemlidir. Preanalitik, analitik, postanalitik süreçlerin uygun şekildegerçekleştirilmesi moleküler yöntemlerde doğru sonuç elde edebilmek için bir başka gerekliliktir. Bu bölümde, güvenli bir moleküler patoloji laboratuvarının fiziksel olarak yapılanmasının ve donanımının nasıl olması gerektiği ile kontaminasyondan kaçınmakiçin yapılması gerekenler ve preanalitik, analitik,postanalitik süreçlerde kalite yönetiminden bahsedilmektedir.</p

An investigation of the relationship between matriptase-2 protein expression and histopathological data in breast cancer

Breast cancer is the most common cancer in females. Therefore, early recognition of the disease is essential. Matriptase-2, a member of the Type II Transmembrane Serine Proteases (TTSP) family, plays a role in various types of cancer (breast, prostate, etc.). The matriptase-2 level is thought to be related to patients' prognosis. This study used the tumor and the normal breast tissue samples from 21 female patients with breast cancer. The levels of matriptase-2 protein expression in the tumor and the normal tissues of the breast cancer patients and examined the relation with histopathological findings were analyzed. The matriptase-2 protein expression levels was determined using the Western Blot method. The matriptase-2 protein expression levels in the tumor tissues were significantly lower than in the normal tissues (p = 0,012). The matriptase-2 protein expression levels in the normal tissues of patients with nuclear grades I/II, histological grades I/II, clinical stages III/IV, presence of lymph nodes, and microcalcification were statistically significantly higher than the matriptase-2 protein expression levels tumor tissues (p = 0,0001 for all). The results of our study are in line with findings in the literature showing that the increase in matriptase-2 level is associated with a good prognosis. Decrease in the level of the matriptase-2 protein in the tumor tissues and the relationship of this decrease with poor prognosis suggests that matriptase-2 may play a role in limiting the development of breast tumors by the mobile and invasive nature of breast cancer cells. The matriptase-2 level may have a prognostic value in breast cancer patients