Vitronectin and Its Receptors Partly Mediate Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium in vitro

International audienc

Initial formation of IGROV1 ovarian cancer multicellular aggregates involves vitronectin

International audienc

Towards an Embedded Integrated Measurement System for the In Vitro Monitoring ofBiomedical Impedance Related to Fibrosis Phenomenon

International audienceIn this article a full wireless integrated measurement system of biomedical impedance is presented with main objective to characterize fibrosis phenomenon using impedance monitoring. It has further scope to make it secure and accurate by transferring measured data wirelessly using a Zigbee protocol This microcontroller-based system acquires the impedance by exciting an electrode and processes data in order to get clear values of real and imaginary data separately. The communication between master and slave is done with I2C protocol. Change in impedance can give a proper idea to reveal an intensity of fibrosis by correlated it to cell abnormal proliferation rate. In this article, as a proof of feasibility, we demonstrate on low and medium range resistors that our system is able to make reliable wireless transfer of impedance data, in accordance with the constraint of future in vitro experiments

Ovarian cancer ascites-derived vitronectin and fibronectin: Combined purification, molecular features and effects on cell response

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A biomimetic model of 3D fluid extracellular macromolecular crowding microenvironment fine-tunes ovarian cancer cells dissemination phenotype

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Synthesis and antitumor activities investigation of a C-nucleoside analogue of ribavirin

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Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification

Abstract At a crime scene, investigators are faced with a multitude of traces. Among them, biological traces are of primary interest for the rapid genetic-based identification of individuals. “Touch DNA” consists of invisible biological traces left by the simple contact of a person’s skin with objects. To date, these traces remain undetectable with the current methods available in the field. This study proposes a proof-of-concept for the original detection of touch DNA by targeting cell-derived fragments in addition to DNA. More specifically, adhesive-structure proteins (laminin, keratin) as well as carbohydrate patterns (mannose, galactose) have been detected with keratinocyte cells derived from a skin and fingermark touch-DNA model over two months in outdoor conditions. Better still, this combinatory detection strategy is compatible with DNA profiling. This proof-of-concept work paves the way for the optimization of tools that can detect touch DNA, which remains a real challenge in helping investigators and the delivery of justice

In Vitro Models of Ovarian Cancer: Bridging the Gap between Pathophysiology and Mechanistic Models

Ovarian cancer (OC) is a disease of major concern with a survival rate of about 40% at five years. This is attributed to the lack of visible and reliable symptoms during the onset of the disease, which leads over 80% of patients to be diagnosed at advanced stages. This implies that metastatic activity has advanced to the peritoneal cavity. It is associated with both genetic and phenotypic heterogeneity, which considerably increase the risks of relapse and reduce the survival rate. To understand ovarian cancer pathophysiology and strengthen the ability for drug screening, further development of relevant in vitro models that recapitulate the complexity of OC microenvironment and dynamics of OC cell population is required. In this line, the recent advances of tridimensional (3D) cell culture and microfluidics have allowed the development of highly innovative models that could bridge the gap between pathophysiology and mechanistic models for clinical research. This review first describes the pathophysiology of OC before detailing the engineering strategies developed to recapitulate those main biological features

Evaluation of the potential of a new ribavirin analog impairing the dissemination of ovarian cancer cells

International audienceEpithelial ovarian cancers are insidious pathologies that give a poor prognosis due to their late discovery and the increasing emergence of chemoresistance. Development of small pharmacological anticancer molecules remains a major challenge. Ribavirin, usually used in the treatment of hepatitis C virus infections and more recently few cancers, has been a suggestion. However, Ribavirin has many side-effects, suggesting that the synthesis of analogs might be more appropriate. We have investigated the effect of a Ribavirin analog, SRO-91, on cancer cell behavioral characteristics considered as some of the hallmarks of cancer. Two human ovarian adenocarcinoma cell lines (SKOV3 and IGROV1) and normal cells (mesothelial and fibroblasts) have been used to compare the effects of SRO-91 with those of Ribavirin on cell behavior underlying tumor cell dissemination. SRO-91, like Ribavirin, inhibits proliferation, migration, clonogenicity and spheroids formation of cancer cells. Unlike Ribavirin, SRO-91 is preferentially toxic to cancer compared with normal cells. An in vitro physiologically relevant model showed that SRO-91, like Ribavirin or cisplatin, inhibits cancer cell implantation onto peritoneal mesothelium. In conclusion, SRO-91 analog effects on tumor dissemination and its safety regarding non-cancerous (normal) cells are encouraging findings a promising drug for the treatment of ovarian cancer