Board 121: Development of a Create-a-Lego-Engineer Activity to Examine Students’ Engineering Identity

This paper describes the development of a Create-a-Lego-Engineer (CALE) activity which was created as an alternative to the Draw-a-Scientist (DAST) and Draw-an-Engineer Tests (DAET). While the DAST and DAET examine students’ (mis)conceptions of scientists and engineers, they provide limited information about whether students can envision themselves as scientists or engineers now or in the future. We drew from the Lego Serious Play (LSP) method which is grounded on the premise that hands-on learning results in a deeper understanding of the world and oneself in it. The LSP method is a process used to enhance innovation and business development, and it involves adults building metaphorical representations of their identity using Lego bricks. We adapted this process for use with elementary students (3rd-5th grade) in a specific context, namely students are asked to build themselves as engineers and a scene depicting what they would be doing as an engineer. Lego bricks were chosen as they are familiar to most students, are easy to use even without prior experience, and require no special skills or artistic abilities. The activity allows us to explore students’ creations of physical representations of themselves as engineers, including issues related to gender and physical characteristics (e.g., skin color, hair color and style), all of which students can customize using a variety of Lego options. Students are provided with a variety of Lego person pieces in order to try and build a representation of themselves using Legos. Additionally, a wide variety of Lego brick pieces were provided in order to allow for numerous ways in which students might represent engineers doing engineering work. Students were asked to imagine themselves as engineers and then to create their Lego engineer. Next, on a notecard, they described the type of work their Lego engineer would be doing, at which point they were then asked to create this scene using Lego bricks. Finally, after completing their creations, students reflected on the meaning of what they built and verbally described their creation and the choices they made. While these reflections provide additional insight into students’ beliefs about who can be an engineer and what engineers do, they also provide students an opportunity to imagine and see themselves in the role of an engineer. This activity was developed within the context of a multi-year, NSF-funded research project examining the dynamics between undergraduate outreach providers and elementary students to understand the impact of the program on students’ engineering identity and career aspirations. This paper will describe the development of the activity as well as preliminary findings from pilot testing and use with elementary students participating in the overall research project. Potential implications and limitations will be described

WNK1-OSR1 kinase-mediated phospho-activation of Na+-K+-2Cl- cotransporter facilitates glioma migration

Background: The bumetanide (BMT)-sensitive Na+-K+-2Cl- cotransporter isoform 1 (NKCC1) maintains cell volume homeostasis by increasing intracellular K+ and Cl- content via regulatory volume increase (RVI). Expression levels of NKCC1 positively correlate with the histological grade and severity of gliomas, the most common primary adult brain tumors, and up-regulated NKCC1 activity facilitates glioma cell migration and apoptotic resistance to the chemotherapeutic drug temozolomide (TMZ). However, the cellular mechanisms underlying NKCC1 functional up-regulation in glioma and in response to TMZ administration remain unknown. Methods: Expression of NKCC1 and its upstream kinases With-No-K (Lysine) kinase 1 (WNK1) and oxidative stress-responsive kinase-1 (OSR1) in different human glioma cell lines and glioma specimens were detected by western blotting and immunostaining. Live cell imaging and microchemotaxis assay were applied to record glioma cell movements under different treatment conditions. Fluorescence indicators were utilized to measure cell volume, intracellular K+ and Cl- content to reflect the activity of NKCC1 on ion transportation. Small interfering RNA (siRNA)-mediated knockdown of WNK1 or OSR1 was used to explore their roles in regulation of NKCC1 activity in glioma cells. Results of different treatment groups were compared by one-way ANOVA using the Bonferroni post-hoc test in the case of multiple comparisons. Results: We show that compared to human neural stem cells and astrocytes, human glioma cells exhibit robust increases in the activation and phosphorylation of NKCC1 and its two upstream regulatory kinases, WNK1 and OSR1. siRNA-mediated knockdown of WNK1 or OSR1 reduces intracellular K+ and Cl- content and RVI in glioma cells by abolishing NKCC1 regulatory phospho-activation. Unexpectedly, TMZ activates the WNK1/OSR1/NKCC1 signaling pathway and enhances glioma migration. Pharmacological inhibition of NKCC1 with its potent inhibitor BMT or siRNA knockdown of WNK1 or OSR1 significantly decreases glioma cell migration after TMZ treatment. Conclusion: Together, our data show a novel role for the WNK1/OSR1/NKCC1 pathway in basal and TMZ-induced glioma migration, and suggest that glioma treatment with TMZ might be improved by drugs that inhibit elements of the WNK1/OSR1/NKCC1 signaling pathway

Board 120: Development of an Engineering Identity and Career Aspirations Survey for Use with Elementary Students

Interest in science, technology, engineering, and mathematics (STEM) begins as early as elementary and middle school. As youth enter adolescence, they begin to shape their personal identities and start making decisions about who they are and could be in the future. Students form their career aspirations and interests related to STEM in elementary school, long before they choose STEM coursework in high school or college. Much of the literature examines either science or STEM identity and career aspirations without separating out individual sub-disciplines. Therefore, the purpose of this paper is to describe the development of a survey instrument to specifically measure engineering identity and career aspirations in adolescents and preadolescents. When possible, we utilized existing measures of STEM identity and career aspirations, adapting them when necessary to the elementary school level and to fit the engineering context. The instrument was developed within the context of a multi-year, NSF-funded research project examining the dynamics between undergraduate outreach providers and elementary students to understand the impact of the program on students’ engineering identity and career aspirations. Three phases of survey development were conducted that involved 492 elementary students from diverse communities in the United States. Three sets of items were developed and/or adapted throughout the four phases. The first set of items assessed Engineering Identity. Recent research suggests that identity consists of three components: recognition, interest, and performance/competence. Items assessing each of these constructs were included in the survey. The second and third sets of items reflected Career Interests and Aspirations. Because elementary and middle school students often have a limited or nascent awareness of what engineers do or misconceptions about what a job in science or engineering entails, it is problematic to measure their engineering identity or career aspirations by directly asking them whether they want to be a scientist/engineer or by using a checklist of broad career categories. Therefore, similar to other researchers, the second set of items assessed the types of activities that students are interested in doing as part of a future career, including both non-STEM and STEM (general and engineering-specific) activities. These items were created by the research team or adapted from activity lists used in existing research. The third set of items drew from career counseling measures relying on Holland’s Career Codes. We adapted the format of these instruments by asking students to choose the activity they liked the most from a list of six activities that reflected each of the codes rather than responding to their interest about each activity. Preliminary findings for each set of items will be discussed. Results from the survey contribute to our understanding of engineering identities and career aspirations in preadolescent and adolescent youth. However, our instrument has the potential for broader application in non-engineering STEM environments (e.g., computer science) with minor wording changes to reflect the relevant science subject area. More research is needed in determining its usefulness in this capacity

“Maybe If I Put My Mind To It : 5th Graders’ Receptivity to Pursuing Engineering Careers (Fundamental)

As educators strive to broaden representation in engineering, it is important to take into account how youth perceive themselves in relation to engineering careers. Youth as young as ten years of age are assessing the appeal and achievability of engineering as a career. This study explores preadolescents’ perceptions of the desirability of engineering careers and the self-assessed characteristics which impact students’ interest in engineering. In particular, this work unpacks what attracts elementary students to engineering careers and what these students believe it takes to be an engineer. Drawing from a set of 56 student interviews, this work addresses the research question In what ways are elementary school students thinking about careers in engineering? Existing research indicates that students’ interest in engineering careers declines as students enter middle school; this study contributes to understanding influences on students’ interests when they are on the cusp of deciding whether to pursue engineering study and careers. As part of a study of a university-led engineering education outreach program in elementary classrooms, 5th grade students participated in 15- to 30-minute semi-structured interviews near the end of the academic year. Participants represented four classrooms in two suburban schools in the northeastern United States that had each engaged in 16-18 weeks of hands-on engineering activities led by undergraduate engineering students. Interviews focused on the students’ experiences with engineering and the engineering intervention, students’ role models, and students’ career aspirations. We utilized open coding to analyze the interviews and identified the keywords and themes that students used to describe why an engineering career would be appealing or unappealing and possible or impossible for them. Two themes characterized the interview data; these students’ receptivity to pursuing engineering careers appeared to be mediated by conceptions of engineering careers as involving skill (in idea generation or in creation of technology) or desire (the will to do engineering). This paper utilizes interview data to attend to students’ perspectives and expand our understanding of barriers and gateways to student interest in engineering study and careers. It also discusses the implications of the findings for teaching engineering with an eye on the messages conveyed about the nature of engineering practices, the characteristics of engineers, and how students’ interests intersect with engineering careers

Analogues of ERβ ligand chloroindazole exert immunomodulatory and remyelinating effects in a mouse model of multiple sclerosis

Abstract Pharmaceutical agents currently approved for the treatment of multiple sclerosis reduce relapse rates, but do not reverse or prevent neurodegeneration nor initiate myelin repair. The highly selective estrogen receptor (ER) β ligand chloroindazole (IndCl) shows particular promise promoting both remyelination while reducing inflammatory cytokines in the central nervous system of mice with experimental autoimmune encephalomyelitis. To optimize these benefits, we developed and screened seven novel IndCl analogues for their efficacy in promoting primary oligodendrocyte (OL) progenitor cell survival, proliferation, and differentiation in vitro by immunohistochemistry. Two analogues, IndCl-o-chloro and IndCl-o-methyl, induced proliferation and differentiation equivalent to IndCl and were selected for subsequent in vivo evaluation for their impact on clinical disease course, white matter pathology, and inflammation. Both compounds ameliorated disease severity, increased mature OLs, and improved overall myelination in the corpus callosum and white matter tracts of the spinal cord. These effects were accompanied by reduced production of the OL toxic molecules interferon-γ and chemokine (C-X-C motif) ligand, CXCL10 by splenocytes with no discernable effect on central nervous system-infiltrating leukocyte numbers, while IndCl-o-methyl also reduced peripheral interleukin (IL)−17. In addition, expression of the chemokine CXCL1, which is associated with developmental oligodendrogenesis, was upregulated by IndCl and both analogues. Furthermore, callosal compound action potential recordings from analogue-treated mice demonstrated a larger N1 component amplitude compared to vehicle, suggesting more functionally myelinated fibers. Thus, the o-Methyl and o-Chloro IndCl analogues represent a class of ERβ ligands that offer significant remyelination and neuroprotection as well as modulation of the immune system; hence, they appear appropriate to consider further for therapeutic development in multiple sclerosis and other demyelinating diseases

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