糖尿病性腎症におけるアルブミンの全ネフロン濾過量および再吸収量の増加に関する研究

京都大学0048新制・課程博士博士(医学)甲第20263号医博第4222号新制||医||1020(附属図書館)京都大学大学院医学研究科医学専攻(主査)教授 長船 健二, 教授 小川 修, 教授 福原 俊一学位規則第4条第1項該当Doctor of Medical ScienceKyoto UniversityDFA

Topology optimization of nonlinear optical waveguide devices considering output signal phase

A signal output of optical devices with multi-input ports, such as a logic gate, depends on the phase difference between input ports. In order to cascade optical devices, it is essential to design such devices considering not only desired output power but also desired output signal phase. We propose a topology optimization method for optical devices considering the output signal phase. In our approach, the beam propagation method (BPM) is employed as a numerical simulation method and the adjoint variable method (AVM) is used to calculate the sensitivity of output power and signal phase to design parameters. The validity and the effectiveness of our approach are verified through design examples of a three-branching waveguide with linear media and an all-optical logic gate utilizing nonlinear media

Minimalist Design of Wireframe DNA Nanotubes: Tunable Geometry, Size, Chirality, and Dynamics

DNA nanotubes (NTs) have attracted extensive interest as artificial cytoskeletons for biomedical, synthetic biology, and materials applications. Here, we report the modular design and assembly of a minimalist yet robust DNA wireframe nanotube with tunable cross-sectional geometry, cavity size, chirality, and length, while using only four DNA strands. We introduce an h-motif structure incorporating double-crossover (DX) tile-like DNA edges to achieve structural rigidity and provide efficient self-assembly of h-motif-based DNA nanotube (H-NT) units, thus producing programmable, micrometer-long nanotubes. We demonstrate control of the H-NT nanotube length via short DNA modulators. Finally, we use an enzyme, RNase H, to take these structures out of equilibrium and trigger nanotube assembly at a physiologically relevant temperature, underlining future cellular applications. The minimalist H-NTs can assemble at near-physiological salt conditions and will serve as an easily synthesized, DNA-economical modular template for biosensors, plasmonics, or other functional materials and as cost-efficient drug-delivery vehicles for biomedical applications.A minimalist design of robust DNA nanotubes from only 4 DNA strands, with tunable geometry, chirality, length, and assembly dynamics is reported. The method leads to robust nanotubes under physiologically relevant conditions, as cost-efficient nanomaterial templates or drug-delivery vehicles.+imag

Molecular diagnosis of an infant with TSC2/PKD1 contiguous gene syndrome

A 1-month-old Japanese infant with cardiac rhabdomyoma was diagnosed with TSC2/PKD1 contiguous gene syndrome by targeted panel sequencing with subsequent quantitative polymerase chain reaction that revealed gross monoallelic deletion, including parts of two genes: exons 19–42 of TSC2 and exons 2–46 of PKD1. Early molecular diagnosis can help to detect bilateral renal cyst formation and multidisciplinary follow-up of this multisystem disease

Gene Expression Profiles of the Cochlea and Vestibular Endorgans: Localization and Function of Genes Causing Deafness

Objectives: We sought to elucidate the gene expression profiles of the causative genes as well as the localization of the encoded proteins involved in hereditary hearing loss. Methods: Relevant articles (as of September 2014) were searched in PubMed databases, and the gene symbols of the genes reported to be associated with deafness were located on the Hereditary Hearing Loss Honnepage using localization, expression, and distribution as keywords. Results: Our review of the literature allowed us to systematize the gene expression profiles for genetic deafness in the inner ear, clarifying the unique functions and specific expression patterns of these genes in the cochlea and vestibular endorgans. Conclusions: The coordinated actions of various encoded molecules are essential for the normal development and maintenance of auditory and vestibular function.ArticleANNALS OF OTOLOGY RHINOLOGY AND LARYNGOLOGY. 124:6S-48S (2015)journal articl