Polymorphisms in the MBL2 gene are associated with the plasma levels of MBL and the cytokines IL-6 and TNF-α in severe COVID-19

IntroductionMannose-binding lectin (MBL) promotes opsonization, favoring phagocytosis and activation of the complement system in response to different microorganisms, and may influence the synthesis of inflammatory cytokines. This study investigated the association of MBL2 gene polymorphisms with the plasma levels of MBL and inflammatory cytokines in COVID-19.MethodsBlood samples from 385 individuals (208 with acute COVID-19 and 117 post-COVID-19) were subjected to real-time PCR genotyping. Plasma measurements of MBL and cytokines were performed by enzyme-linked immunosorbent assay and flow cytometry, respectively.ResultsThe frequencies of the polymorphic MBL2 genotype (OO) and allele (O) were higher in patients with severe COVID-19 (p< 0.05). The polymorphic genotypes (AO and OO) were associated with lower MBL levels (p< 0.05). IL-6 and TNF-α were higher in patients with low MBL and severe COVID-19 (p< 0.05). No association of polymorphisms, MBL levels, or cytokine levels with long COVID was observed.DiscussionThe results suggest that, besides MBL2 polymorphisms promoting a reduction in MBL levels and therefore in its function, they may also contribute to the development of a more intense inflammatory process responsible for the severity of COVID-19

Fatores de risco e proteção contra doenças crônicas não transmissíveis entre adolescentes

Objective: To know the prevalence of risk factors for noncommunicable diseases (NCDs) among adolescents assisted by the municipal program “Health and Prevention at School” (Saúde e Prevenção na Escola – SPE). Method: A cross-sectional study was carried out with students aged 10 to 17 years from six schools located in the municipality of Imperatriz, Maranhão, Brazil, between October 2014 and April 2015. Anthropometric measures were collected at the school and variables related to eating habits and lifestyle were investigated using a self-administered questionnaire. Data were compared between genders using the Chi-squared test. Results: A total of 473 students aged 10-17 years were assessed. Overweight and obesity occurred in 13.3% (63) of the sample. The consumption of foods such as rice, beans and fruits is associated with protective factors. There was a higher consumption of these foods among girls (p=0.001) and physical activity was prevalent among boys (p=0.0001). There are no differences in exposure to cigarettes, illicit drugs and alcohol between genders. Conclusion: The study population presents different patterns of exposure to risk and protective factors and there is an evident need for educational actions in the school environment conducted by SPE professionals and other school managers.Objetivo: Conocer la prevalencia de los factores de riesgo para las enfermedades crónicas no transmisibles (ECNT) entre adolescentes asistidos por el programa municipal Salud y Prevención en la Escuela (SPE). Métodos: Estudio transversal realizado con escolares entre 10 y 17 años de seis escuelas del municipio de Imperatriz, Maranhão, Brasil, entre octubre de 2014 y abril de 2015. Las medidas antropométricas fueron recogidas en la propia escuela y las variables asociadas con los hábitos alimentarios y el estilo de vida fueron investigadas a través de un cuestionario auto-aplicado. Los datos fueron comparados entre los sexos a través de la prueba Chi-Cuadrado. Resultados: Fueron evaluados 473 estudiantes entre 10 y 17 años. El sobrepeso y la obesidad se dieron en el 3,3% (63) de la muestra. Alimentos como el arroz, los frijoles y las frutas tienen el consumo asociado con factores de protección y presentan mayores proporciones de consumo entre las niñas (p= 0,001) mientras la práctica de actividad física prevalece entre los niños (p = 0, 0001). No hay diferencia entre los sexos para la exposición al tabaco, las drogas ilícitas y el alcohol. Conclusión: La población estudiada presenta distintos patrones de exposición para los factores de riesgo y protección y es evidente la necesidad de acciones educativas en el ambiente de la escuela que sean conducidas por los profesionales del SPE y demás gestores de las escuelas.Objetivo: Conhecer a prevalência dos fatores de risco para doenças crônicas não transmissíveis (DCNT) entre adolescentes assistidos pelo programa municipal Saúde e Prevenção na Escola (SPE). Métodos: Estudo de corte transversal realizado com escolares de 10 a 17 anos pertencentes a seis escolas do município de Imperatriz, Maranhão, Brasil, entre outubro de 2014 e abril de 2015. Medidas antropométricas foram coletadas na própria escola e as variáveis associadas aos hábitos alimentares e estilo de vida foram investigadas por meio de questionário autoaplicável, sendo comparados os dados entre os sexos através do teste Qui-quadrado. Resultados: Foram avaliados 473 estudantes entre 10 e 17 anos. O sobrepeso e a obesidade ocorrem em 13,3% (63) da amostra. Alimentos como arroz, feijão e frutas têm consumo associado a fatores de proteção e apresentaram maiores proporções de consumo entre o sexo feminino (p= 0,001), enquanto a prática de atividade física prevalece entre o sexo masculino (p = 0, 0001). A exposição ao cigarro, drogas ilícitas e álcool não se distingue entre os sexos. Conclusão: A população estudada apresenta distintos padrões de exposição a fatores de risco e proteção, e evidencia a necessidade de ações educativas no ambiente escolar conduzidas pelos profissionais do SPE e demais gestores escolares

Prevalence and Risk Factors for HTLV-1/2 Infection inRiverside and Rural Populations of the State of Pará

Human T-lymphotropic viruses 1 and 2 (HTLV-1 and HTLV-2) infection has been described in several Amazonian populations; however, there is still a lack of data on the prevalence of the virus in riparian populations living in rural areas of the state of Pará. The present study aimed to evaluate the prevalence of HTLV-1/2 infection in four riverine communities and one rural area in the state of Pará and to describe the possible risk factors for infection. A total of 907 individuals responded to an epidemiological survey and gave blood samples collected for anti-HTLV-1/2 antibodies by immunoenzymatic assay (EIA). The serum-reactive samples were subjected to confirmation by an in-line assay (Inno-Lia) and by proviral DNA screening using real-time PCR (qPCR). The total prevalence was 0.8% (7/907) for HTLV-1/2 (CI: 0.2−1.3%), with 0.66% HTLV-1 and 0.11% HTLV-2. The prevalence by sex was 0.7% in women (4/565) and 0.9% in men (3/342). Among seropositive patients, 83.3% (5/7) reported being sexually active, and 57.1% (4/7) reported not having the habit of using condoms during their sexual relations. Intrafamily infection was also observed. The results reinforce the need for public policies to prevent and block the spread of HTLV, especially in riparian communities that are subject to difficulties in accessing the Unified Health System (Sistema Único de Saúde/SUS) because infected individuals need clinical monitoring for surveillance and early diagnosis of symptoms associated with HTLV-1

HTLV-1/2 in Indigenous Peoples of the Brazilian Amazon: Seroprevalence, Molecular Characterization and Sociobehavioral Factors Related to Risk of Infection

HTLV-1/2 infection is endemic in Indigenous peoples of the Americas. Its origin is attributed to the migratory flow of Amerindian ancestral peoples. The present study aimed to investigate the seroprevalence of HTLV-1/2 infection in Indigenous peoples of the Brazilian Amazon. A total of 3350 Indigenous people belonging to 15 communities were investigated. The investigation was performed using serological (ELISA), molecular (qPCR) and confirmatory (Western blot and/or Inno-Lia) tests to detect and differentiate the infection. The seroprevalence was 8.3% for HTLV-1/2 infection, with 0.1% of individuals seropositive for HTLV-1 and 8.1% for HTLV-2. The prevalence of infection was statistically higher in women (10.1%) than in men (6.5%) (p = 0.0002). This female predominance was observed in all age groups; in females the prevalence was significant from 41 years old (p p < 0.0001). Here, we present a prevalence of HTLV-1/2 among Indigenous peoples of the Brazilian Amazon. The endemic infection in these groups must reflect the different epidemiological profiles observed in these peoples, such as sexual transmission through rejection of condom use, breastfeeding, especially in cases of cross-breastfeeding, and the high rate of pregnancy in the villages

Polymorphisms in the MBL2 gene are associated with the plasma levels of MBL and the cytokines IL-6 and TNF-α in severe COVID-19

National Council for Scientific and Technological Development (CNPQ #401235/2020-3); Fundação Amazônia de Amparo a Estudos e Pesquisa do Pará (FAPESPA #005/2020 and #006/2020), Secretaria de Estado de Ciência, Tecnologia e Educação Profissional e Tecnológica (#09/ 2021) and Universidade Federal do Pará (PAPQ/2022)Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Laboratório de Pesquisa Básica em Malária, Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Laboratório de Pesquisa Básica em Malária, Ananindeua, PA, Brasil / Federal University of Pará. Institute of Medical Sciences. School of Medicine. Belém, PA, Brazil.Belém Adventist Hospital. Belém, PA, Brazil.Belém Adventist Hospital. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Laboratório de Imunologia. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Laboratório de Imunologia. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Laboratório de Imunologia. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Laboratório de Imunologia. Ananindeua, PA, Brasil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Ciência, Tecnologia, Inovação e Insumos Estratégicos. Instituto Evandro Chagas. Programa de Pós-Graduação em Virologia. Ananindeua, PA, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde e Ambiente. Instituto Evandro Chagas. Laboratório de Imunologia. Ananindeua, PA, Brasil.University of the State of Pará. Center of Biological and Health Sciences. Belém, PA, Brazil.University of the State of Pará. Center of Biological and Health Sciences. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Laboratory of Genetics of Complex Diseases. Belém, PA, Brazil.Federal University of Pará. Institute of Biological Sciences. Laboratory of Virology. Belém, PA, Brazil / Federal University of Pará. Institute of Biological Sciences. Graduate Program in Biology of Infectious and Parasitic Agents. Belém, PA, Brazil.Introduction: Mannose-binding lectin (MBL) promotes opsonization, favoring phagocytosis and activation of the complement system in response to different microorganisms, and may influence the synthesis of inflammatory cytokines. This study investigated the association of MBL2 gene polymorphisms with the plasma levels of MBL and inflammatory cytokines in COVID-19. Methods: Blood samples from 385 individuals (208 with acute COVID-19 and 117 post-COVID-19) were subjected to real-time PCR genotyping. Plasma measurements of MBL and cytokines were performed by enzyme-linked immunosorbent assay and flow cytometry, respectively. Results: The frequencies of the polymorphic MBL2 genotype (OO) and allele (O) were higher in patients with severe COVID-19 (p< 0.05). The polymorphic genotypes (AO and OO) were associated with lower MBL levels (p< 0.05). IL-6 and TNF-α were higher in patients with low MBL and severe COVID-19 (p< 0.05). No association of polymorphisms, MBL levels, or cytokine levels with long COVID was observed. Discussion: The results suggest that, besides MBL2 polymorphisms promoting a reduction in MBL levels and therefore in its function, they may also contribute to the development of a more intense inflammatory process responsible for the severity of COVID-19