Conditioned medium from stem cells derived from human exfoliated deciduous teeth ameliorates NASH via the Gut-Liver axis

Non-alcoholic steatohepatitis (NASH) occurrence has been increasing and is becoming a major cause of liver cirrhosis and liver cancer. However, effective treatments for NASH are still lacking. We examined the benefits of serum-free conditioned medium from stem cells derived from human exfoliated deciduous teeth (SHED-CM) on a murine non-alcoholic steatohepatitis (NASH) model induced by a combination of Western diet (WD) and repeated administration of low doses of carbon tetrachloride intraperitoneally, focusing on the gut-liver axis. We showed that repeated intravenous administration of SHED-CM significantly ameliorated histological liver fibrosis and inflammation in a murine NASH model. SHED-CM inhibited parenchymal cell apoptosis and reduced the activation of inflammatory macrophages. Gene expression of pro-inflammatory and pro-fibrotic mediators (such as Tnf-α, Tgf-β, and Ccl-2) in the liver was reduced in mice treated with SHED-CM. Furthermore, SHED-CM protected intestinal tight junctions and maintained intestinal barrier function, while suppressing gene expression of the receptor for endotoxin, Toll-like receptor 4, in the liver. SHED-CM promoted the recovery of Caco-2 monolayer dysfunction induced by IFN-γ and TNF-α in vitro. Our findings suggest that SHED-CM may inhibit NASH fibrosis via the gut-liver axis, in addition to its protective effect on hepatocytes and the induction of macrophages with unique anti-inflammatory phenotypes

〈再録論文〉Fabrication of the Plasma-Chemical Indicator Strip for the Detection of Plasma Processing Effects

［Abstract］ In order to confirm the plasma processing effect at a required point by visual observation, the plasma-chemical indicator (PCI) was fabricated. PCI is the piece of PET (polyethylene terephthalate) sheet strip on which a color indication mark which is sensitive and distinguishable to H_2O_2 and O_2, plasma was printed. The distinguishable property on PCI between H_2O_2, andO_2 plasma was realized by mixing two different colorants: red azo-dye and green phthalocyanine pigment were mixed into nitro cellulose resin. When PCI is treated by H_2O_2 and H_2O plasma, the color is continuously shifted from the purple of control PCI toward dull light green and light bluish green respectively after 15 minutes of treatment time. However, when the PCI is treated by O_2 plasma, it also continuously shifted toward a pinkish color after 15 minutes of treatment time. Therefore, it could be said that this prepared PCI is sensitive and distinguishable to H_2O_2 and O_2 plasma. Finally it is discovered that not only final color, but also the color in the middle of the change can also be available as PCI.[再録元]Journal of Photopolymer Science and Technology Volume25,Nember4(2012)539-544 (C) 2012CPS