Development of a simple information pump.

The Information Pump (IP) is a methodology that aims to counter the problems arising from traditional subjective product data collection. The IP is a game theory based process that aims to maximise information extracted from a panel of subjects, while maintaining their interest in the process through a continuous panelist scoring method. The challenge with implementing this arises from the difficulty in executing the 'game'. In its original format, there is an assumption that the game is played with each player using their own PC to interact with the process. While this in theory allows information and scores to flow in a controlled manner between the players, it actually provides a major barrier to the wider adoption of the IP method. This barrier is two-fold: it is costly and complex, and it is not a natural manner for exchanging information. The core objective is to develop a low cost version of the IP method. This will use the game theory approach to maintain interest among participants and maximise information extraction, but remove the need for each participant to have their own PC interface to the game. This will require replacing both the inter-player communication method and the score keeping/reporting

Yeast Two-Hybrid: State of the Art

Genome projects are approaching completion and are saturating sequence databases. This paper discusses the role of the two-hybrid system as a generator of hypotheses. Apart from this rather exhaustive, financially and labour intensive procedure, more refined functional studies can be undertaken. Indeed, by making hybrids of two-hybrid systems, customised approaches can be developed in order to attack specific function-related problems. For example, one could set-up a "differential" screen by combining a forward and a reverse approach in a three-hybrid set-up. Another very interesting project is the use of peptide libraries in two-hybrid approaches. This could enable the identification of peptides with very high specificity comparable to "real" antibodies. With the technology available, the only limitation is imagination

Methods for high-dimensonal analysis of cells dissociated from cyropreserved synovial tissue

Background: Detailed molecular analyses of cells from rheumatoid arthritis (RA) synovium hold promise in identifying cellular phenotypes that drive tissue pathology and joint damage. The Accelerating Medicines Partnership RA/SLE Network aims to deconstruct autoimmune pathology by examining cells within target tissues through multiple high-dimensional assays. Robust standardized protocols need to be developed before cellular phenotypes at a single cell level can be effectively compared across patient samples. Methods: Multiple clinical sites collected cryopreserved synovial tissue fragments from arthroplasty and synovial biopsy in a 10% DMSO solution. Mechanical and enzymatic dissociation parameters were optimized for viable cell extraction and surface protein preservation for cell sorting and mass cytometry, as well as for reproducibility in RNA sequencing (RNA-seq). Cryopreserved synovial samples were collectively analyzed at a central processing site by a custom-designed and validated 35-marker mass cytometry panel. In parallel, each sample was flow sorted into fibroblast, T-cell, B-cell, and macrophage suspensions for bulk population RNA-seq and plate-based single-cell CEL-Seq2 RNA-seq. Results: Upon dissociation, cryopreserved synovial tissue fragments yielded a high frequency of viable cells, comparable to samples undergoing immediate processing. Optimization of synovial tissue dissociation across six clinical collection sites with ~ 30 arthroplasty and ~ 20 biopsy samples yielded a consensus digestion protocol using 100 μg/ml of Liberase™ TL enzyme preparation. This protocol yielded immune and stromal cell lineages with preserved surface markers and minimized variability across replicate RNA-seq transcriptomes. Mass cytometry analysis of cells from cryopreserved synovium distinguished diverse fibroblast phenotypes, distinct populations of memory B cells and antibody-secreting cells, and multiple CD4+ and CD8+ T-cell activation states. Bulk RNA-seq of sorted cell populations demonstrated robust separation of synovial lymphocytes, fibroblasts, and macrophages. Single-cell RNA-seq produced transcriptomes of over 1000 genes/cell, including transcripts encoding characteristic lineage markers identified. Conclusions: We have established a robust protocol to acquire viable cells from cryopreserved synovial tissue with intact transcriptomes and cell surface phenotypes. A centralized pipeline to generate multiple high-dimensional analyses of synovial tissue samples collected across a collaborative network was developed. Integrated analysis of such datasets from large patient cohorts may help define molecular heterogeneity within RA pathology and identify new therapeutic targets and biomarkers

Development of a simple information pump

The Information Pump (IP) is a methodology that aims to counter the problems arising from traditional subjective product data collection. The IP is a game theory based process that aims to maximise information extracted from a panel of subjects, while maintaining their interest in the process through a continuous panelist scoring method. The challenge with implementing this arises from the difficulty in executing the 'game'. In its original format, there is an assumption that the game is played with each player using their own PC to interact with the process. While this in theory allows information and scores to flow in a controlled manner between the players, it actually provides a major barrier to the wider adoption of the IP method. This barrier is two-fold: it is costly and complex, and it is not a natural manner for exchanging information. The core objective is to develop a low cost version of the IP method. This will use the game theory approach to maintain interest among participants and maximise information extraction, but remove the need for each participant to have their own PC interface to the game. This will require replacing both the inter-player communication method and the score keeping/reporting

The Evolutionary Significance of Red Sox Nation: Sport Fandom as a Byproduct of Coalitional Psychology

Sport fandom has received considerable attention from social scientists, yet few have considered it from an evolutionary perspective. To redress this gap, we develop the hypothesis that team sports exhibit characteristics that activate mechanisms which evolved to facilitate the development of coalitions in the context of small-scale warfare. Based on this by-product hypothesis, we predicted a correlation between fandom and binding (i.e. group-relevant) concerns, especially loyalty. To test this prediction, we administered the Sport Spectator Identification Scale (SSI) and the Moral Foundations Questionnaire (MFQ) to 495 undergraduates. The MFQ measures three binding concerns, including loyalty, and two individualizing ones, harm and fairness. As predicted, fandom correlated significantly with loyalty ( r = .27) and, within men, the two other binding concerns, authority ( r =.22) and purity ( r = .24). By contrast, fandom did not significantly correlate with harm or fairness. In addition, we predicted and found that men reported significantly higher levels of fandom (Cohen's d =.45) and loyalty ( d = .27) than did women. In conclusion, this study presents data supporting the coalitional by-product hypothesis of fandom and should spur further research using fandom as a window into our evolved psychology