Molecular characterization and expression of DgZFP1, a gene encoding a single zinc finger protein in chrysanthemum

A single zinc finger protein gene was isolated from chrysanthemum by rapid amplification of cDNA ends (RACE) approach and was designated as DgZFP1. The DgZFP1 encodes a protein of 168 amino acids residues with a calculated molecular mass of 18.1 kDa and theoretical isoelectric point is 4.71. DgZFP1 contains one single zinc finger motif and one ethylene-responsive element-binding factor (ERF)-associated amphiphilic repression (EAR) domain. The transcripts of DgZFP1 was enriched in nodes and ray petal than in disc petal, disc stamen, disc pistil and ray pistil, but not detected in other tissues. Subcellular localization revealed that DgZFP1 was preferentially distributed to nucleus. We argued that DgZFP1 is a new member of the single zinc finger protein genes and it may be the ortholog of LIF

Isolation and molecular characterization of RcSERK1: A Rosa canina gene transcriptionally induced during initiation of protocorm-like bodies

A somatic embryogensis receptor-like kinase (SERK) gene was isolated from protocorm-like bodies (PLBs) of Rosa canina by a rapid amplification of cDNA ends (RACE) approach and was designated as RcSERK1. The RcSERK1 encodes a protein of 626 amino acid residues with a calculated molecular mass of 68.79 kDa and theoretical isoelectric point of 5.65. The amino acid sequence of RcSERK1 shares all the characteristic features of a SERK protein, including the signal peptide (SP), the leucine zipper (LZ), the five leucine-rich repeats (LRRs), the pro-rich domain containing the so-called Ser-Pro- Pro (SPP) motif, the transmembrane domain (TM), the kinase domain and the C-terminal domain. The transcripts of RcSERK1 were more enriched in PLBs than in rhizoids and callus, but not detected in leaflets (incubated under dark and before producing callus) and the regenerated shoots. Subcellular localization indicated that the fluorescence of RcSERK1-GFP was recorded in the plasma membrane. We argue that RcSERK1 is a Leu-rich repeat receptor-like kinase (LRR-RLK) and plasma  membrane localization protein.Keywords: somatic embryogensis receptor-like kinase (SERK)1, protocorm-like bodies (PLBs), Rosa canina, RACE, RcSERK1

Hypothetical model for host searching and selection processes of fall armyworm females and larvae (neonate and older), based on literature sources and this study’s results (see Discussion for literature sources).

<p>This study’s findings are pertinent to various stages of the searching and selection processes, so were incorporated into the model as hypotheses for further study, and are indicated by filled boxes containing italicized text.</p