Development and Validation of Stability Indicating RP-HPLC Method for Estimation of Lorcaserin Hydrochloride in Bulk and Tablet Dosage Form

In the current study a simple, precise, sensitive and accurate reversed phase liquid chromatography method was developed for the analysis and estimation of Lorcaserin HCL in bulk and tablet dosage form. The present study of Lorcaserin HCL was achieved by using Cosmosil C18 (250nm×4.6ID, Particle Size: 5 Micron) Column with mobile phase Methanol:10mM KH2PO4 Buffer (70:30) pH:3 at a flow rate 0.8ml/min with UV detection at 222nm. The retention time for Lorcaserin HCL was found to be 5.108 min. In Linearity the correlation coefficient (R2) for Lorcaserin HCL was found to be 0.9995, slope is 42071 and intercept was found to be 21966 which are well within the acceptance criteria. The mean percent recovery for Lorcaserin HCL at three different levels for 50%, 100%, and 150% was found to be 100.65%, 98.84% and 100.34%. The %RSD (NMT 2%). In precision study interday (RSD is 0.26%) and intraday (RSD is 0.29%) are found.  Forced degradation experiments was carried out by exposing standard form of Lorcaserin HCL for Acid-base hydrolytic, Oxidative, photolytic and thermal stress conditions. The method has been validated by System suitability parameters, Linearity, Accuracy and Percent recovery, Precision, Ruggedness, Robustness, LOD and LOQ. Keywords: Lorcaserin hydrochloride, RP-HPLC, Validation

GSTP1 rs1695 polymorphism, oxidative stress markers, and antioxidants in coronary artery disease

Oxidative damage is among the essential factors in the progression of cardiovascular disease. Objectives: This study aims to evaluate the molecular role of GST genotypic polymorphism involved in the development of CAD. This study also aimed to compare the levels of oxidative stress and antioxidant markers in subjects with CAD with age and sex-matched controls. Result: There was no significant difference in allele frequency (p= 0.85) or genotype frequency (p= 0.85) between the examined case and control groups. Compared to healthy controls, F2-Isoprostanes and MDA levels were considerably elevated in individuals with coronary artery disease. CAD patients' GST, SOD, Vitamine E, and Vitamin C levels were considerably lower than in normal control subjects. Conclusion: This study observed that oxidative stress markers were significantly higher, whereas, in CAD patients, enzymatic and nonenzymatic-enzymatic antioxidants were significantly lower. This study could not find a good connection between GSTP1 gene polymorphism rs1695 and coronary artery disease

LDLR Gene Polymorphisms in CAD Patients Among South-Indian Tamil Population

Introduction and aim: Coronary artery disease (CAD) is a multifactorial condition caused by the interplay of hereditary and environmental factors. The major objective of this research is to evaluate the Low-density of lipoprotein receptor (LDLR) single nucleotide polymorphisms (SNPs) rs688 and rs5925 and the lipid profile in coronary artery disease (CAD) and their connection with CAD. Materials and methods: The research included 50 patients with coronary artery disease and 30 healthy controls. To examine the lipid profile parameters, Fasting venous blood was taken. Genomic DNA was isolated, amplified, and identified using an allele-specific polymerized chain reaction for the genetic study of LDLR SNPs. Results: Lipid profile parameters were shown to be substantially (p0.000) linked with coronary artery disease. There was, however, no significant change in the allele and genotype frequencies of the LDLR SNPs rs688 and rs5925 between the case and control groups investigated. Conclusion: The current investigation was unable to detect a significant connection between the LDLR gene SNPs rs688 and rs5925 with CAD

GSTP1 Rs1695 Polymorphism, Oxidative Stress Markers, and Antioxidants in Coronary Artery Disease

Oxidative damage is among the essential factors in the progression of cardiovascular disease. Objectives: This study aims to evaluate the molecular role of GST genotypic polymorphism involved in the development of CAD. This study also aimed to compare the levels of oxidative stress and antioxidant markers in subjects with CAD with age and sex-matched controls. Result: There was no significant difference in allele frequency (p= 0.85) or genotype frequency (p= 0.85) between the examined case and control groups. Compared to healthy controls, F2-Isoprostanes and MDA levels were considerably elevated in individuals with coronary artery disease. CAD patients' GST, SOD, Vitamine E, and Vitamin C levels were considerably lower than in normal control subjects. Conclusion: This study observed that oxidative stress markers were significantly higher, whereas, in CAD patients, enzymatic and nonenzymatic-enzymatic antioxidants were significantly lower. This study could not find a good connection between GSTP1 gene polymorphism rs1695 and coronary artery disease

LDLR gene polymorphisms in CAD patients among South-Indian Tamil population

Introduction and aim: Coronary artery disease (CAD) is a multifactorial condition caused by the interplay of hereditary and environmental factors. The major objective of this research is to evaluate the Low-density of lipoprotein receptor (LDLR) single nucleotide polymorphisms (SNPs) rs688 and rs5925 and the lipid profile in coronary artery disease (CAD) and their connection with CAD. Materials and methods: The research included 50 patients with coronary artery disease and 30 healthy controls. To examine the lipid profile parameters, fasting venous blood was taken. Genomic DNA was isolated, amplified, and identified using an allele-specific polymerized chain reaction for the genetic study of LDLR SNPs. Results: Lipid profile parameters were shown to be substantially (p0.000) linked with coronary artery disease. There was, however, no significant change in the allele and genotype frequencies of the LDLR SNPs rs688 and rs5925 between the case and control groups investigated. Conclusion: The current investigation was unable to detect a significant connection between the LDLR gene SNPs rs688 and rs5925 with CAD

Stability-Indicating Reverse-Phase High-Performance Liquid Chromatographic Method Development and Validation of Lamotrigine in Bulk and Pharmaceutical Dosage Form

In the current study, analytical method has been validated by system suitability parameters, linearity, accuracy and percent recovery, precision, ruggedness, robustness, limit of detection, and limit of quantification. The present study of lamotrigine was achieved using Cosmosil C18 (250 nm×4.6 ID, particle size: 5 Micron) Column with mobile phase methanol: water (60:40) pH: 3 at a flow rate 0.8 ml/min with UV detection at 308 nm. The retention time for lamotrigine was found to be 4.979 min. In linearity, the correlation coefficient (R2) for lamotrigine was found to be 0.999, slope is 39,801, and intercept was found to be 51,862 which are well within the acceptance criteria. The mean percent recovery for lamotrigine at three different levels for 50%, 100%, and 150% was found to be 99.28%, 99.30%, and 100.40%. The % RSD should not more than 2%. In precision study, interday (RSD is 0.41%) and intraday (RSD is 0.26%) are found. Forced degradation experiments were carried out by exposing standard form of lamotrigine for acid-base, oxidative, photolytic, and thermal stress conditions. Hence, the developed method is accurate, precise, repeatable, and reproducible and can be used for routine analysis of lamotrigine