The laminin-keratin link shields the nucleus from mechanical deformation and signalling

The mechanical properties of the extracellular matrix dictate tissue behaviour. In epithelial tissues, laminin is a very abundant extracellular matrix component and a key supporting element. Here we show that laminin hinders the mechanoresponses of breast epithelial cells by shielding the nucleus from mechanical deformation. Coating substrates with laminin-111-unlike fibronectin or collagen I-impairs cell response to substrate rigidity and YAP nuclear localization. Blocking the laminin-specific integrin β4 increases nuclear YAP ratios in a rigidity-dependent manner without affecting the cell forces or focal adhesions. By combining mechanical perturbations and mathematical modelling, we show that β4 integrins establish a mechanical linkage between the substrate and keratin cytoskeleton, which stiffens the network and shields the nucleus from actomyosin-mediated mechanical deformation. In turn, this affects the nuclear YAP mechanoresponses, chromatin methylation and cell invasion in three dimensions. Our results demonstrate a mechanism by which tissues can regulate their sensitivity to mechanical signals.© 2023. The Author(s)

The force loading rate drives cell mechanosensing through both reinforcement and cytoskeletal softening.

Cell response to force regulates essential processes in health and disease. However, the fundamental mechanical variables that cells sense and respond to remain unclear. Here we show that the rate of force application (loading rate) drives mechanosensing, as predicted by a molecular clutch model. By applying dynamic force regimes to cells through substrate stretching, optical tweezers, and atomic force microscopy, we find that increasing loading rates trigger talin-dependent mechanosensing, leading to adhesion growth and reinforcement, and YAP nuclear localization. However, above a given threshold the actin cytoskeleton softens, decreasing loading rates and preventing reinforcement. By stretching rat lungs in vivo, we show that a similar phenomenon may occur. Our results show that cell sensing of external forces and of passive mechanical parameters (like tissue stiffness) can be understood through the same mechanisms, driven by the properties under force of the mechanosensing molecules involved

The laminin–keratin link shields the nucleus from mechanical deformation and signalling

The mechanical properties of the extracellular matrix dictate tissue behaviour. In epithelial tissues, laminin is a very abundant extracellular matrix component and a key supporting element. Here we show that laminin hinders the mechanoresponses of breast epithelial cells by shielding the nucleus from mechanical deformation. Coating substrates with laminin-111—unlike fibronectin or collagen I—impairs cell response to substrate rigidity and YAP nuclear localization. Blocking the laminin-specific integrin ß4 increases nuclear YAP ratios in a rigidity-dependent manner without affecting the cell forces or focal adhesions. By combining mechanical perturbations and mathematical modelling, we show that ß4 integrins establish a mechanical linkage between the substrate and keratin cytoskeleton, which stiffens the network and shields the nucleus from actomyosin-mediated mechanical deformation. In turn, this affects the nuclear YAP mechanoresponses, chromatin methylation and cell invasion in three dimensions. Our results demonstrate a mechanism by which tissues can regulate their sensitivity to mechanical signals.We thank A. Farré and the other members of IMPETUX OPTICS, S.L., for their help and expertise in the design and implementation of the optical tweezers experiments; R. Sunyer for help and advice with the microprinting experiments; S. Usieto, A. Menéndez, N. Castro, M. Purciolas and W. Haaksma for providing technical support; L. Rosetti and S. Saloustros for providing data analysis tools; and J. de Rooij, A. L. Le Roux, L. Faure, A. Labernadie, R. Oria and J. Abenza, as well as all the members of the groups of P.R.-C. and X.T. for helpful discussion. Finally, we thank G. Wiche, A. Sonnenberg and N. Montserrat for providing plasmids, antibodies or cell lines used for this work. We acknowledge funding from the Spanish Ministry of Science and Innovation (PID2021-128635NB-I00 MCIN/AEI/10.13039/501100011033 and ‘ERDF-EU A way of making Europe’ to X.T., PID2019-110949GB-I00 to M.A. and PID2019-110298GB-I00 to P.R.-C.), the European Commission (H2020-FETPROACT-01-2016-731957), the European Research Council (Adv-883739 to X.T.; CoG-681434 to M.A.; StG- 851055 to A.E.-A.), the Generalitat de Catalunya (2017-SGR-1602 to X.T. and P.R.-C.; 2017-SGR-1278 to M.A. and P.S.) and European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 797621 to M.G.-G. The prize ‘ICREA Academia’ for excellence in research to M.A. and P.R.-C., Fundació la Marató de TV3 (201936-30-31 and 201903-30-31-32), and ‘la Caixa’ Foundation (LCF/PR/HR20/52400004 and ID 100010434 under agreement LCF/PR/HR20/52400004). IBEC and CIMNE are recipients of a Severo Ochoa Award of Excellence from MINCIN. A.E.M.B. was supported by a Sir Henry Wellcome Fellowship (210887/Z/18/Z). A.E.-A. receives funding from the Francis Crick Institute, which receives its core funding from the Cancer Research UK (CC2214), the UK Medical Research Council (CC2214) and the Wellcome Trust (CC2214).Peer ReviewedPostprint (published version

The laminin-keratin link shields the nucleus from mechanical deformation and signalling

The mechanical properties of the extracellular matrix dictate tissue behaviour. In epithelial tissues, laminin is a very abundant extracellular matrix component and a key supporting element. Here we show that laminin hinders the mechanoresponses of breast epithelial cells by shielding the nucleus from mechanical deformation. Coating substrates with laminin-111-unlike fibronectin or collagen I-impairs cell response to substrate rigidity and YAP nuclear localization. Blocking the laminin-specific integrin β4 increases nuclear YAP ratios in a rigidity-dependent manner without affecting the cell forces or focal adhesions. By combining mechanical perturbations and mathematical modelling, we show that β4 integrins establish a mechanical linkage between the substrate and keratin cytoskeleton, which stiffens the network and shields the nucleus from actomyosin-mediated mechanical deformation. In turn, this affects the nuclear YAP mechanoresponses, chromatin methylation and cell invasion in three dimensions. Our results demonstrate a mechanism by which tissues can regulate their sensitivity to mechanical signals

Cytoskeletal Safeguards: Protecting the Nucleus from Mechanical Perturbations

The cell nucleus plays a key role in cellular mechanoresponses. 3D genome organisation, gene expression, and cell behaviour, in general, are affected by mechanical force application to the nucleus, which is transmitted from the cellular environment via a network of interconnected cytoskeletal components. To effectively regulate cell responses, these cytoskeletal components must not only exert forces but also withstand external forces when necessary. This review delves into the latest research concerning how the cytoskeleton safeguards the nucleus from mechanical perturbations. Specifically, we focus on the three primary cytoskeletal polymers: actin, intermediate filaments, and microtubules, as well as their interactions with the cell nucleus. We discuss how the cytoskeleton acts as a protective shield for the nucleus, ensuring structural integrity and conveying context-specific mechanoresponses