Pseudo-Dolly-In Video Generation Combining 3D Modeling and Image Reconstruction

This paper proposes a pseudo-dolly-in video generation method that reproduces motion parallax by applying image reconstruction processing to multi-view videos. Since dolly-in video is taken by moving a camera forward to reproduce motion parallax, we can present a sense of immersion. However, at a sporting event in a large-scale space, moving a camera is difficult. Our research generates dolly-in video from multi-view images captured by fixed cameras. By applying the Image-Based Modeling technique, dolly-in video can be generated. Unfortunately, the video quality is often damaged by the 3D estimation error. On the other hand, Bullet-Time realizes high-quality video observation. However, moving the virtual-viewpoint from the capturing positions is difficult. To solve these problems, we propose a method to generate a pseudo-dolly-in image by installing 3D estimation and image reconstruction techniques into Bullet-Time and show its effectiveness by applying it to multi-view videos captured at an actual soccer stadium. In the experiment, we compared the proposed method with digital zoom images and with the dolly-in video generated from the Image-Based Modeling and Rendering method.Published in: 2017 IEEE International Symposium on Mixed and Augmented Reality (ISMAR-Adjunct) Date of Conference: 9-13 Oct. 2017 Conference Location: Nantes, Franc

The Escherichia coli transcriptome linked to growth fitness

A series of Escherichia coli strains with varied genomic sequences were subjected to high-density microarray analyses to elucidate the fitness-correlated transcriptomes. Fitness, which is commonly evaluated by the growth rate during the exponential phase, is not only determined by the genome but is also linked to growth conditions, e.g., temperature. We previously reported genetic and environmental contributions to E. coli transcriptomes and evolutionary transcriptome changes in thermal adaptation. Here, we describe experimental details on how to prepare microarray samples that truly represent the growth fitness of the E. coli cells. A step-by-step record of sample preparation procedures that correspond to growing cells and transcriptome data sets that are deposited at the GEO database (GSE33212, GSE52770, GSE61739) are also provided for reference. Keywords: Transcriptome, Growth fitness, Escherichia coli, Microarra

Real‐world clinical practice of pulmonary arterial hypertension in Japan: Insights from a large administrative database

Abstract Pulmonary arterial hypertension (PAH) is a fatal disease that often occurs at an early age. In recent years, aggressive treatment with multiple drugs from the early‐stage diagnosis is expected to improve the prognosis. Indeed, a high rate of initial combination therapy and excellent treatment outcomes have been reported from specialized centers for PAH in Japan. However, information on PAH epidemiology, including non‐PAH specialized centers in Japan, is unclear. To address the above, we conducted a retrospective observational cohort study from April 2008 to September 2020 using real‐world evidence from a large‐scale administrative database (Medical Data Vision) to examine baseline characteristics, comorbidities, and treatment profiles of Japanese patients with PAH. Five hundred and eighteen patients with PAH (treatment‐naive PAH, age 67.2 ± 15.9) were identified through our comprehensive approach which combined PAH disease codes, medications, and diagnostic procedures. Moreover, we showed that a larger proportion of patients received monotherapy in their initial treatment (66%) compared to those receiving combination therapy (34%). During the 1‐year follow‐up after PAH diagnosis, 13% of patients increased their PAH medications while other patients either decreased their PAH medications (6%) or discontinued PAH treatment (27%). The 3‐ and 5‐year event‐free survival rates of all‐cause death were 72% and 64%, respectively. This is the first large‐scale administrative database study that provides insights into real‐world PAH management in Japan. This study highlighted a different PAH clinical landscape which included a larger portion of the elderly population, higher initial monotherapy treatment, and lower survival rates than previous studies

Fragment Molecular Orbital Based Interaction Analyses on Complexes Between RBD Variants and ACE2

The spike protein plays an important role in the infection of SARS-CoV-2 to human cells, and the binding affinity of receptor binding domain (RBD) to angiotensin-converting enzyme 2 (ACE2) is of special interest. In this report, we present a series of interaction analyses for the RBD - ACE2 complex (PDB ID: 6M0J) and mutated complexes of UK (B.1.1.7 lineage), South Africa (B1.351) and Brazil (B1.1.248) types, based on the fragment molecular orbital (FMO) calculations. The effects of mutations are investigated in terms of inter-fragment interaction energies (IFIEs), indicating the higher affinities of RBD variants with ACE2