Hydrology and hydrobiology and environmental pollutions in lower than 10 meters depths of Caspian Sea

The present project in related to survey of factors and hydrology and hydrochemical features (water temperature, dissolve oxygen saturation, pH, clearance, salinity, nitrogen, phosphorus and silicon) hydrobiology (zooplankton, phytoplankton, macrobenthos) and survey of bio environment pollution (oil, heavy metal, detergent) executed in lower 10m in different water larger in southern Caspian Sea in 2002-2003. For sampling 8 lines number were vertical on coast that selected from Astra in west to Gomishan in east in southern Caspian Sea basin. The result indicated the average physical factors such as pH were 8.11 and salinity12.12 ppt ,and disolve oxygen6.7 mg/l. Average chemical factors such as NO_2 , NO_3 and NH_4 were 1.2 µg/l, 25.7 µg/l, 13 µg/l respectively.Total nitogen and organic nitrogen and inorganic nitrogen were 690.2 µg/l , 667.6 µg/l , 41.6 µg/l. Average silicat were recrded 266.35 µg/l . Total Phosphorus was observed 37.35 µg/l and average of organic Phosphorus concentration was and 20.25 µg/l .Average of Total organic matter (T.O.M) was 4.98% maximum amount were observed in Lisar and minimum in Nooshahr . Concentration of heavy metal during sampling were respectively ,Fe>Mn>Zn>Cr>Ph>Co>Cd>Cu. Maximum concentration of Fe were determined in winter in Nooshahr and Babolsar respectively 13/3 µg/l 17/1 µg/l. In many stations and different Season, the amount of heavy meta were lower standard of in marine water. The concentration of oil hydrocarborate (PAHs) in autumn was 0/13 ppb and in winter 0/12 ppb. The amount of (PAHs) in Southern Caspian Sea were Lower than other parts of Caspian Sea. The average of detergent concentration (LAS) was 0/036 µg/l that was two fold higher than determined in 2001. Total 107 species of phytoplankton belong to 5 phylum were identified. The numbers of species of phytoplankton groups were respectively, chrysophyta (42 species), cyanophyta (17 species), pyruphyta (17 species), chlorophyta (21 species) and euglenophyta (9 species). The maximum diversity of phytoplankton observed in summer and minimum in autumn. High diversity of chrysophta and cyanophyta observed in summer and phyrophyta and chrlophyta in spring. The composition of phytoplanhkton groups were respectively, chrysophyta (70%), phyrophyta (9%) and chlorophyta (7%) and euglonophyta (1%). Maximum density of phytoplankton was observed in autumn and minimum in winter. Total 19 species of Zooplankton were identified. Maximum diversity was observed in summer and minimum in winter. Zooplankton changes during sampling, showed amount of density of zooplankton in 5m were more than 10 m depths. Total (17 species macrobenthos were identified. The composition of macrobenthos groups were respectively , Annalida (92/7% ) , Bivalvia (2/7%) gumarida (108%) cumacea ( 1/5%) , Balanidae 103% . max . density were observed in Astara and min . in Sefied roud Average of density were 1218 0/851 ind /m^2 and biomass 14 15 g/m^2 High density were recorded in autumn and low density in winter . Correlation of phytoplankton and zooplankton with physicochemical parameter and also relation between total organic matter and sediment grain size were calculated.Ecological indicies (simpson diversity evenns diversity and shanoon-wiever diversity) were calculated for macrobenthos. Data were shown impact of cetenephora (Mnenemiopsis leidyi) on zooplankton and phytoplankton and macrobenthos density

Limnologic study Sabalan run off Dam for aquaculture fish cold water

Limnological study of Sabalan run off Damfor aquaculture cold water fish was conducted in sixtimesat four stations from July to December 2009. Average minimum and maximum parameters measured in different stations were included water temperature, dissolved Oxygen, total hardness, electrical conductivity, pH, BOD5, COD, TDS, NH4, NO2, NO3, PO4, TN, TP, SiO2, respectively (10/3±9/5-14±8/7°C), (9±1/2-11/4±2/1 mg/liter), (367±19/8-381±17/8mg/liter), (0/95±0/07-1/05±0/07 mz/cm), (8/3±0/1-8/4±0/1), (3/3±1/14-3/88 ± 0/71mg/ liter), (15/5 ± 3/1-16/3 ± 3 mg/liter), (0/48 ± 0/04-0/54 ± 0/06mg/ liter), (0/08 ± 0/02-0/13 ± 0/07 mg/liter), (0/004 ± 0/003- 0/05 ± 0/1 mg/ liter), (0/6 ± 0/2-0/64 ± 0/29 mg/ liter), (0/19 ± 0/07-0/22 ± 0/07 mg/ liter), (10/48 ± 0/6-10/98 ± 0/54mg/liter), (0/39 ± 0/11-0/44 ± 0/18 mg/liter), and(9/9 ±0/6-11/1 ± 1/4 mg/liter). Average minimum and maximum of water heavy metalswas included of Cd, Pb, Zn, Fe, Cuand Ni respectively was measured as ND(No Deliver), ND, ND- 0/242, ND- 1/075, ND- 0/018 and ND-0/014inmg/ liter. Also among the 16 organochlorinecom pounds measured the highest amount of was belonged totoxin Hexachlorine Benzene (1/5 mg/ liter). The results of the analysis of water quality parameters showed that parameters except ofCOD, TDS, PO4, TP, Zn, Fe and water temperature, other parameters do not haveany restrictions foraquaculturecold water fish in therunoffDam. Based on data results it was noticed that there was temperature restriction up to November at downstream and station 2 showed less temperature fluctuation and more suitable condition compared to other stations. BOD5 value was within the allowable range for growing trout, in which station 2 somewhat with lower volatility of value had the most suitable BOD5 condition. In the present study, the amount of TDS in the study area was somewhat higher than the limitation and since TDS values increased with distance from the dam, therefore station 2 had somewhat better conditions for fish farming. In addition the amount of EC in the study area, indicating the inappropriateness of it for various activities in the fisheries. Although none of the stations in terms of the amount of phosphorus didn't show favorable conditions for rainbow trout fish propagation, it appears between the researches stations, station 2 had the most favorable terms. The results of this study (regardless of the unseen), it seems that in August and September; Zn had overestimated value for cold fish propagation. Recent study showed that among heavy metals, Fe in water, sediments and fish was over limitation value. Also, Fe value was measured of 1.08 at station 4, which showed over normal value for cold fish propagation. Therefore, station 2 at downstream seemed to have suitable cold fish propagation due to lower Fe value. On the based on the recent study, planktonic biological index did not endorse water quality for fish propagation. In this study, aquatic plant such as Potamogeton pectinatus and Pheragmitie saustralis was found at water of higher and somehow polluted organic matter. Probably, based on the results it was noticed that downstream water of dam was polluted to organic matter. At the recent study, resisted maroinvertebrates to organic matter (Chironomidae) was consisted noticeable frequency compared to other sensitive invertebrates to organic matter (EPT).Statistically, it can be expressed that probably organic pollution resulted to increase of resistance invertebrates and decreased of sensitive groups. In recent study, EPT/CHIR index was measured 0.6 in station 2 and 1.74 in station 4. This ration was less to many other mountain rivers. As ETP sensitive invertebrates has higher sensitive to other groups with the regards of environmental condition and pollution, unpredicted increase of Chironomidae compare to sensitive groups resulted of EPT/CHIR value which showed environmental stress. In this study, HFBI index was measured as 7.49 at station 2 and 7 in station 4. Based on HFBI index, station 2 was classified as a high organic pollution and station 4 with noticeable organic pollution. At the recent study, station 2 was catch more fish to station4. For instance, Squaliuscephalus and Alburnoides pinctatus were catch in station 2 and were not found in station 4. As a whole, Biological indicators whichwas included Plankton Bioindicator, EPT / CHIR. ,Hilsenh off Biological Indicator, diversity and distribution ofaquatic plants and fish, showed water situation in run off Damrich inorganic matter. Finally, the study area was approved for fish farm about 50 tons and needs management arrangements with the increased production rate

Designing and establishment of ISO/IEC 17025 in 3aboratories of Caspian Sea ecological research center

The project was carried out between March of 2007 and November of 2009.Five laboratories of research center (Clinical pathology, Plankton, Molecular genetics, Pollutants, Analysis Instrument were selected for accreditation. The main stages for establishment of the system consisted of: 1-Conducting a gap analysis to compare the present state of the laboratories with ISO/IEC 17025 Training 2 General requirements for the competence of testing and calibration laboratories Validation of methods Estimation of uncertainty Internal audits 3- Performing of technical and management requirements 4-Submit of quality manual to Iran Accreditation System (IAS) in order to accredit In January of 2010 the laboratories were accredited by Iran Accreditation System (IAS). The main results were including: 1-Increase the accuracy of measurement 2-Improvement of the Repeatability and Reproducibility of the test methods 3-Traceability and standardization of test methods 4- Calibration of measurement instruments 6- Updating of test methods 7-Standardization of physical condition of the laboratories 8- Getting the certification from Iran Accreditation System (IAS)