The Effects of Leukocyte- and Platelet-Rich Plasma (L-Prp) and Pure Platelet-Rich Plasma (P-Prp) in a Rat Endometriosis Model

Objective: We aimed to investigate the effect of platelet‑rich plasma (PRP) derivatives, which can be produced from the patient’s blood and have minimal side effects, on endometriosis.Methods: To our knowledge, this is the first study in the literature that studies the relationship between PRP and endometriosis. Endometriosis foci were created in the first operation. In the second operation (30th day), four groups were formed wherein group 1 (n = 8) was administered saline, group 2 (n = 7) leukocyte and platelet‑rich plasma (L‑PRP), group 3 (n = 8) pure platelet‑rich plasma (P‑PRP) and group 4 (n = 10) was used to obtain PRP. In the last operation (60th day), the endometriotic foci was measured and then excised.Findings: There was no statistically significant difference between the pre and post volumes of the endometriotic foci, between their volume differences, and volume difference rates (P > 0.05). However, it was observed that existing implant volumes in all groups decreased statistically significantly within their groups by the end of the experiment compared to the previous volumes (P < 0.05).Conclusion: When the implants were assessed through histopathological scoring in terms of edema, vascular congestion, inflammatory cell  infiltration, hemorrhage, epithelial line, and hemosiderin accumulation, and immunohistochemical staining was assessed in terms of VEGF, there was no significant difference in the comparison between the groups. Although L‑PRP and P‑PRP generated more reduction in the endometriosis foci, they did not create any statistical differences. Key words: L‑Prp; P‑Prp; VEGF; endometriosis

Mitokondriyal GST Kappa1’in mesane kanserindeki protein ekspresyonunun incelenmesi

Tez (Yüksek Lisans) -- Kırıkkale Üniversitesi111024

Examination of mitokondrial GST Kappa1 isoenzyme protein expressios in bladder cancer

YÖK Tez ID: 444305Glutation-S-transferaz (GST) enzim ailesi hücrelerin hemen hemen her türünde mevcuttur ve birçok reaksiyonlarda ve endojen maddelerin metabolizmasında çok önemli bir rol oynar. Bu enzim sistemi ilaçlar, çevre kirliliği ve kanserojen biyotransformasyonunda çok önemlidir. Bu çalışmada, mesane kanseri patogenezinde, GSTK1 izoenziminin olası etkisini immünohistokimyasal boyama ile araştırdık. Yapılan çalışmada altmış mesane papiller ürotelyal karsinom vakasında, GST Kappa (K1) izoziminin immünohistokimyasal bulguları değerlendirildi. Bu hastalara ait dokular boyanma şiddetine göre karşılaştırıldığında; Mesane karsinomada GSTK1 izoziminin protein ekspresyonunun tümörlü dokularda normal dokulara oranla daha fazla olduğu bulunmuştur (p=0,0000; 0,0000,05). Bu bulgulara göre GSTK1 izoziminin mesane kanserinde diagnostik olarak önemi olabileceği söylenebilir.Glutathione-S-transferase (GST) enzyme family is present in almost all type of cells and can play a critical role in several reactions and metabolism of endogen materials. This enzyme system is important for drugs, enviromental pollution and carcinogen biotransformation. In this study, we investigated GSTK1 isoezyme with immunohistochemical staining for possible effect on the patogenesis of bladder cancer. GST Kappa (K1) isozyme immunohistochemical finding was evaluated in sixty papillary urothelial bladder carcinoma cases. When the tissues of these cases were compared according to their staining intensity, GSTK1 expression in bladder carcinoma was significantly higher than normal bladder tissues (p=0,0000; 0,0000,05). According to these findings, it is likely to be of importance GSTK1 isozyme in the diagnosis of bladder cancer

Expression of Sigma-Class Glutathione-S-Transferase in Fetal and Pediatric Filum Terminale Samples: A Comparative Study

Background and objectives: The pathophysiology of tethered cord syndrome (TCS) in children is not well elucidated. An inelastic filum terminale (FT) is the main factor underlying the stretching of the spinal cord in TCS. Our study aimed to investigate the expression of glutathione-S-transferase (GST) in children and fetal FT samples in order to understand the relationship between this enzyme expression and the development of TCS. Materials and Methods: FT samples were obtained from ten children with TCS (Group 1) and histological and immunohistochemical examinations were performed. For comparison, FT samples from fifteen normal human fetuses (Group 2) were also analyzed using the same techniques. Statistical comparison was made using a Chi-square test. Results: Positive GST-sigma expression was detected in eight (80%) of 10 samples in Group 1. The positive GST-sigma expression was less frequent in nine (60%) of 15 samples from Group 2. No statistically significant difference was detected between the two groups (p = 0.197). Conclusions: Decreased FT elasticity in TCS may be associated with increased GST expression in FT. More prospective studies are needed to clarify the mechanism of the GST–TCS relationship in children