14 research outputs found
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook
In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise
Chemical profile, acetylcholinesterase, butyrylcholinesterase, and prolyl oligopeptidase inhibitory activity of Glaucium corniculatum subsp. refractum
Papaveraceae is one of the prominent alkaloid-containing families, and plants of the genus Glaucium (Papaveraceae) are known for their bioactive alkaloids. Glaucium species have been used in traditional medicine in Turkey as an analgesic, narcotic, sedative, and antitussive. In this study, it was planned to evaluate the inhibitory activity of an alkaloidal extract of Glaucium corniculatum subsp. refractum on acetylcholinesterase (AChE), butyrylcholinesterase (BuChE) and prolyl oligopeptidase (POP), as well as exploring the chemical profile of the plant by using Gas Chromatography-Mass Spectrometry (GC-MS). The AChE, BuChE and POP inhibition activities of the alkaloidal extract of G. corniculatum subsp. refractum were determined spectrophotometrically. A rapid GC-MS method was used to identify alkaloids that could be responsible for these inhibition activities. In total, eleven alkaloids were identified in the alkaloid extract of the plant by GC-MS. Allocyptopine (52.92%) and protopine (25.38%) were found as the major constituents. The alkaloidal extract of G. corniculatum subsp. refractum showed potent AChE inhibitory activity (IC50:1.25 µg/mL) and BuChE inhibitory activity (IC50: 7.02 µg/mL). The extract also showed a remarkable inhibitory effect on POP with an IC50 value of 123.69 µg/mL. This study presents the first GC-MS investigation and POP inhibitory activity of G. corniculatum subsp. refractum
HPLC - DAD Analysis of Lycorine in Amaryllidaceae Species
WOS: 000278891800010PubMed ID: 20614813Lycorine, the most frequent alkaloid found in Amaryllidaceae plants, has been proven to have various biological activities. Therefore, it is important to quantify this compound in Amaryllidacaeae species. In this study, a reversed-phase high-performance liquid chromatographic method has been used and validated for the determination of lycorine in Amaryllidaceae plants. A simple method for the extraction of lycorine in low-mass plant samples was employed utilizing columns pre-packed with diatomaceous earth (Extrelut (R)). This method was applied to the aerial parts and bulbs of Sternbergia sicula Tineo ex Guss., S. lutea (L.) Ker-Gawl. ex Sprengel and Pancratium maritimum L. (Amaryllidaceae) collected during two different vegetation periods. The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:90:10) applied at a flow rate 1 mL min(-1) using diode array detector. Validation procedures showed that the method was specific, accurate and precise. The amount of lycorine in S. sicula samples ranged between 0.10-0.53%, whereas in those of S. lutea and P. maritimum, the range was 0.19-0.40% and 0.05-0.14%, respectively.Ege UniversityEge University [04-ECZ-023]This study was financially supported by the Ege University Research Fund (Project No: 04-ECZ-023)
Phytochemical investigation of Galanthus woronowii
WOS: 000329421000045Ege University Research FundEge University [09/ECZ/021]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK); EBILTEMEge UniversityThis work was partially supported Ege University Research Fund (09/ECZ/021), TUBITAK and EBILTEM
Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook
WOS: 000400155300003In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut (R)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5,v/v/v) applied at a flow rate 1 mL min(-1) using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise.Ege University Research FundEge University [09/ECZ/037]; TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [104T272]; EBILTEMEge University [2007/BIL/007]This study was financially supported by the Ege University Research Fund (Project No: 09/ECZ/037) and partially supported by TUBITAK (104T272) and EBILTEM (2007/BIL/007)
GC-MS Investigation and Acetylcholinesterase Inhibitory Activity of Galanthus rizehensis
WOS: 000322090700007PubMed ID: 23819307GC-MS (gas chromatography-mass spectrometry) analyses of alkaloids in the aerial parts and bulbs of Galanthus rizehensis Stern (Amaryllidaceae), collected during two different vegetation periods, was performed. Twenty three alkaloids were identified in four different alkaloid extracts. Acetylcholinesterase (AChE) inhibitory activities of the alkaloid extracts were tested. Both the highest alkaloid diversity and the most potent inhibitory activity (IC50 12.94 mu g/ml) were obtained in extracts from the bulbs of G. rizehensis collected during the fruiting period.TUBITAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [TBAG-104T272]; EBILTEMEge University [2007/BIL/007]; Ege University Research FundEge University [09/ECZ/014, 09/ECZ/021]; TUBITAK-BIDEBTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK)This study was financially supported by TUBITAK (TBAG-104T272), EBILTEM (2007/BIL/007), Ege University Research Fund (09/ECZ/014, 09/ECZ/021). B. B. S. thanks TUBITAK-BIDEB for a research fellowship. The authors also thank Dr. Asuncion Marin (Serveis Cientificotecnics, Universitat de Barcelona, Facultat de Farmacia, Barcelona, Spain) for performing the GC-MS analyses