7 research outputs found
Evaluation of the Photostability and Photodynamic Efficacy of Rose Bengal Loaded in Multivesicular Liposomes
Purpose: Rose Bengal (RB) is a potential photodynamic sensitizer in
anticancer therapy. The purpose of this study was to prepare
multivesicular liposomes (MVL) loaded with RB to enhance its
photostability and intracellulaer photodynamic efficacy. Methods:
Four MVL-RB formulations were prepared by reverse phase evaporation
technique using a double emulsion method. The photostability of RB in
phosphate buffer and in MVL was studied by monitoring the change in its
absorption spectra at different time points following photoirradiation
at 550 nm. Photodynamic cytotoxicity, intracellular uptake and
localization of the most photostable MVL-RB were studied on baby
hamster kidney fibroblasts to evaluate its photodynamic efficacy,
compared with free RB. Results: MVL-RB demonstrated significantly
slower photodegradation rates with 10-fold extended halflife compared
with free RB in buffer (p < 0.05). The degradation followed pseudo
first order kinetics. Photodynamic cytotoxicity studies revealed that
MVL-RB increased cell mortality by 1.6 -2.5-fold, compared to free RB,
and this could be attributed to its enhanced intracellular uptake and
different localization pattern in the cell, in addition to increased
photostability. Conclusion: Loading RB in MVL is a promising approach
to improve the photodynamic efficacy of RB, by enhancing its
photostability and delivery into cells
Evaluation of the Photostability and Photodynamic Efficacy of Rose Bengal Loaded in Multivesicular Liposomes
Purpose: Rose Bengal (RB) is a potential photodynamic sensitizer in
anticancer therapy. The purpose of this study was to prepare
multivesicular liposomes (MVL) loaded with RB to enhance its
photostability and intracellulaer photodynamic efficacy. Methods:
Four MVL-RB formulations were prepared by reverse phase evaporation
technique using a double emulsion method. The photostability of RB in
phosphate buffer and in MVL was studied by monitoring the change in its
absorption spectra at different time points following photoirradiation
at 550 nm. Photodynamic cytotoxicity, intracellular uptake and
localization of the most photostable MVL-RB were studied on baby
hamster kidney fibroblasts to evaluate its photodynamic efficacy,
compared with free RB. Results: MVL-RB demonstrated significantly
slower photodegradation rates with 10-fold extended halflife compared
with free RB in buffer (p < 0.05). The degradation followed pseudo
first order kinetics. Photodynamic cytotoxicity studies revealed that
MVL-RB increased cell mortality by 1.6 -2.5-fold, compared to free RB,
and this could be attributed to its enhanced intracellular uptake and
different localization pattern in the cell, in addition to increased
photostability. Conclusion: Loading RB in MVL is a promising approach
to improve the photodynamic efficacy of RB, by enhancing its
photostability and delivery into cells
Zinc octa-n-alkyl phthalocyanines in photodynamic therapy: photophysical properties, accumulation and apoptosis in cell cultures, studies in erythrocytes and topical application to Balb/c mice skin
Two octa-substituted phthalocyanines, namely 1,4,8,11,15,18,22,25-octakis(decyl) phthalocyaninato zinc(II) (ZnODPc) and 1,4,8,11,15,18,22,25-octakis(pentyl) phthalocyaninato zinc(II) (ZnOPPc), were investigated for their use in photodynamic therapy ( PDT) after topical application. Both substances exhibited favourable properties as photosensitisers in vitro: absorption maxima around 700 nm with absorption coefficients of about 190000 (M-1 cm(-1)), a singlet oxygen quantum yield of 0.47 +/- 0.02 (ZnODPc), and good accumulation in keratinocytes and. broblasts. Cell death after phthalocyanine-photosensitisation appeared to occur mainly via apoptosis. The in vivo experiments demonstrated a good accumulation of the phthalocyanines after topical application in a tetrahydrofuran - azone formulation onto the dorsal skin of Balb/c mice: [(4.6 - 4.7) +/- 1.0]% of deposited dye could be recovered after 3 h from deposition. ZnODPc showed significantly better skin-photosensitising properties than ZnOPPc and is therefore a potential candidate for the treatment of psoriasis