Traditional Turkish Coffee with Medicinal Effect

Traditional Turkish coffee (TTC) is highly associated with caffeine and is known as a mind and heart stimulant as it helps keep tiredness at bay. Daily consumption of TTC naturally benefits human health such as anti-cancer, anti-diabetic, improved energy, anti-depression, reduced risk of heart disease, etc. The TTC was derived from particular types of Arabic coffee beans (ACB), and the preparation method of TTC is unique from other types of coffee. The main objective of the study was to investigate the therapeutic and biological effects of TTC. The ACB powder was characterized physicochemically using UV-Vis spectroscopy, Fourier transforms infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). In vitro analysis using HaCaT (Human keratinocyte cell line) proved the biocompatibility of ACB powder. Case studies which were focusing on healthy individuals as the research populace were conducted using TTC. Consumption of TTC was found beneficially compared to other types of coffee. The TTC was obtained from ACB, which was characterized by spectroscopic techniques and displayed biocompatibility due to the results on HaCaT cell lines. The TTC has beneficial therapeutic effects on individuals. According to statistical analysis, the disease-affected ratio of diabetes, heart disease, and depression was significantly decreased

Cell Death Mechanism of Organometallic Ruthenium(II) and Iridium(III) Arene Complexes on HepG2 and Vero Cells

Due to side effects and toxicity associated with platinum-derived metal-based drugs, extensive research has been conducted on ruthenium (Ru) complexes. We aim to synthesize a highly oil soluble Ru(II)-p-cymene complex (Ru1) with an aliphatic chain group, a bimetallic Ru(II)-p-cymene complex (Ru2) with N,S,S triple-coordination and a bimetallic Ir(III)-pentamethylcyclopentadienyl complex (Ir1) with S,S double-coordination. Subsequently, we investigate the effects of these complexes on Vero and HepG2 cell lines, focusing on cell death mechanisms. Characterization of the complexes is performed through nuclear magnetic resonance spectroscopy (1H and 13C NMR) and Fourier-transform infrared spectroscopy. The effective doses are determined using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide) (MTT) assay, applying different doses of the complexes to the two cell lines for 24 and 48 h, respectively. Immunoreactivities of Bax, Bcl2, caspase-3, RIP3, and RIPK1 are analyzed using the indirect immunoperoxidase technique. Notably, all the complexes (Ru1, Ru2, and Ir1) exhibit distinct cell death mechanisms, showing greater effectiveness than cisplatin. This study reveals the diverse mechanisms of action of Ru and Ir complexes based on different ligands. To the best of our knowledge, this study represents the first investigation of a novel RAED-type complex (Ru1) and unexpected bimetallic complexes (Ru2 and Ir1)

Apoptotic Properties of Rutheinum Complexes on Different Type of Cancer Cell Lines

Among chemotherapeutic agents, cisplatin and the other platinum-based drugs have occupied for 35 years an enviable position. The limitations of platinum-based drugs, dose dependent side effects and development of drug resistance mechanisms, have boosted the research for finding other metal-based drugs. Among metals, ruthenium is probably the one showing the greatest promises. Ruthenium (Ru) appears to be less toxic than platinum and several biological studies have indicated that ruthenium complexes possess diverse modes of action. The redox chemistry of ruthenium is rich and compatible with biological media, and the overall toxicity of ruthenium is lower than platinum, thus allowing higher doses of treatment. In this study we aimed that, analyses of different type of ruthenium complexes in cancer cell lines. Six Ru complexes were determined by elemental analysis, FTIR, NMR, UV-visible spectroscopy, electron density on the metal was measured by cyclic voltammetry. After that, the cellular properties of this complexes were analyses on PC-3, HT-29, Du-145 and Vero cell lines. DNA damage was analyzed H2AX staining, apoptotic cell analyses were performed flow cytometry and western blotting. After 48 h incubation of Ru complexes three of them more effective for cell lines. Especially Ru3 was more effective in cancer cell lines. Apoptotic pathway was triggered after Ru complexes incubation in PC-3, Du-145 and Ht-29 cancer cell lines. Our study suggest that Ru complexes may be used for cancer cell cytotoxicity as a drugs in patients

Gelatin/Nanofibrin bioactive scaffold prepared with enhanced biocompatibility for skin tissue regeneration

This research study was to develop a nano bioactive scaffold (NBAS) using gelatins (Gel), nanofibrin (Nano-FB), and glycerol (Gly) by film casting method for potential use in skin tissue engineering. The developed NBAS were characterized for their molecular interaction (Fourier transforms infrared spectroscopy (FTIR)), microstructure (scanning electron microscopy (S.E.M.)), mechanical strength (tensile strength (MPa), elongation at break (%), and flexibility (%)), and Cell viability (M.T.T. assay ((3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide)) were assessed in the biocompatibility study. The mechanical result of the N.B.F. possessed better (tensile strength of 5.22 ± 0.07 MPa), (elongation at break of 5.88 + 0.04%), and (flexibility of 9.18 ± 0.09%) properties. The Invitro study using a human keratinocyte (HaCaT) cell line proved the 100% biocompatibility of NBAS. Based outcome of this study, performed its mechanical properties and exhibited biocompatibility in skin tissue engineering. The study has devised a process for using slaughterhouse and fish waste in the production of valuable medical products like wound dressing materials