Adhesion and invasion of bovine endothelial cells by Neospora caninum

Neospora caninum is a recently identified coccidian parasite which was, until 1988, misdiagnosed as Toxoplasma gondii. It causes paralysis and death in dogs and neonatal mortality and abortion in cattle, sheep, goats and horses. The life-cycle of Neospora has not yet been elucidated. The only two stages identified so far are tissue cysts and intracellularly dividing tachyzoites. Very little is known about the biology of this species. We have set up a fluorescence-based adhesion/invasion assay in order to investigate the interaction of N. caninum tachyzoites with bovine aorta endothelial (BAE) cells in vitro. Treatment of both host cells and parasites with metabolic inhibitors determined the metabolic requirements for adhesion and invasion. Chemical and enzymatic modifications of parasite and endothelial cell surfaces were used in order to obtain information on the nature of cell surface components responsible for the interaction between parasite and host. Electron microscopical investigations defined the ultrastructural characteristics of the adhesion and invasion process, and provided information on the intracellular development of the parasite

Effects of energy dependence in the quasiparticle density of states on far-infrared absorption in the pseudogap state

We derive a relationship between the optical conductivity scattering rate 1/\tau(\omega) and the electron-boson spectral function \alpha^2F(\Omega) valid for the case when the electronic density of states, N(\epsilon), cannot be taken as constant in the vicinity of the Fermi level. This relationship turned out to be useful for analyzing the experimental data in the pseudogap state of cuprate superconductors.Comment: 8 pages, RevTeX4, 1 EPS figure; final version published in PR

Feasibility of Organic Certification of Sheep and Goats Produced in Pastoral Systems in Northern Kenya

In pastoral systems of Kenya, sale of small ruminants is the main regular income source for most households. Although the meat of small ruminants produced in Marsabit county is preferred in the domestic market, no mechanisms are in place that allow for a respective price differentiation. Establishing value chains for labelled sheep and goat meat would be an option to allow pastoralists to profit from their high process and product quality. The aim of the current study is to assess the requirements and feasibility to establish a value chain for labelled products (e.g. origin labelled, organic certified) together with the primary and secondary actors in small ruminant supply chain among the Rendille pastoralists in Marsabit county. Overall, 28 group discussions with four Rendille women income-generating community groups and 21 interviews with other relevant stakeholders were conducted. The results show that pastoralists’ production is largely compatible with organic standards as livestock is fed on naturally growing fodder with no chemicals applied, and herding allows for animals’ natural behaviour. However, a number of challenges hamper organic certification. These include practice of ear notching and castrations without anaesthesia and/or analgesia, supplementary feeding of home-based animals with non-organic feeds during drought, ad-hoc application of veterinary drugs, challenges in record keeping and traceability systems and financial difficulties to undertake internal control and monitoring due to the extensive nature of the production area. Improvement of veterinary infrastructure, innovative record keeping systems for illiterate producers, and further market studies to ascertain demand are recommended. While organic certification is desirable, geographical indication labeling is a feasible starting point that would allow entry into higher priced markets as hurdles to organic certification are addressed

Precise Experimental Investigation of Eigenmodes in a Planar Ion Crystal

The accurate characterization of eigenmodes and eigenfrequencies of two-dimensional ion crystals provides the foundation for the use of such structures for quantum simulation purposes. We present a combined experimental and theoretical study of two-dimensional ion crystals. We demonstrate that standard pseudopotential theory accurately predicts the positions of the ions and the location of structural transitions between different crystal configurations. However, pseudopotential theory is insufficient to determine eigenfrequencies of the two-dimensional ion crystals accurately but shows significant deviations from the experimental data obtained from resolved sideband spectroscopy. Agreement at the level of 2.5 x 10^(-3) is found with the full time-dependent Coulomb theory using the Floquet-Lyapunov approach and the effect is understood from the dynamics of two-dimensional ion crystals in the Paul trap. The results represent initial steps towards an exploitation of these structures for quantum simulation schemes.Comment: 5 pages, 4 figures, supplemental material (mathematica and matlab files) available upon reques

Multiple energetic injections in a strong spike-like solar burst

An intense and fast spike-like solar burst was built up of short time scale structures superimposed on an underlying gradual emission, the time evolution of which shows remarkable proportionality between hard X-ray and microwave fluxes. The finer time structure were best defined at mm-microwaves. At the peak of the event, the finer structures repeat every 30x60ms. The more slowly varying component with a time scale of about 1 second was identified in microwave hard X-rays throughout the burst duration. It is suggested that X-ray fluxes might also be proportional to the repetition rate of basic units of energy injection (quasi-quantized). The relevant parameters of one primary energy release site are estimated both in the case where hard X-rays are produced primarily by thick-target bremsstrahlung, and when they are purely thermal. The relation of this figure to global energy considerations is discussed

Changes in the superconducting properties of high-T(sub c) ceramics produced by applied electric fields

Effect of an electrostatic field in the electrode-insulator-superconductor system on the current-voltage characteristics of high-T(sub c) ceramics with various composition and different preparation technology has been studied at 77 K. Ceramics of Y-Ba-Cu-O (123) and Bi-Pb-Sr-Ca-Cu-O (2223) systems and also ones doped by Ag have been used. Electric field strength has been up to 140 MV/m. It has been shown that there are reversible changes in the critical current I(sub c) and in the conductivity in electric field at the currents somewhat more than I(sub c) at T is less than T(sub c), while at T is greater than T(sub c) the noticeable electric field effect has not been found. These effects are qualitatively similar in both ceramic systems. High negative and positive gate voltages result in an increase of the conductivity. The electric field effect is modified by magnetic field H. The field effect decreases with increasing magnetic field and disappears at H is greater than 30 Oe. In Y-Ba-Cu-O/Ag (10 wt. percent) ceramics the field effect is practically absent. It may be supposed that in the ceramics the field-induced effect is consistent with weak links at grain boundaries

Thermodynamics of the Complex su(3) Toda Theory

We present the first computation of the thermodynamic properties of the complex su(3) Toda theory. This is possible thanks to a new string hypothesis, which involves bound states that are non self-conjugate solutions of the Bethe equations. Our method provides equivalently the solution of the su(3) generalization of the XXZ chain. In the repulsive regime, we confirm that the scattering theory proposed over the past few years - made only of solitons with non diagonal S-matrices - is complete. But we show that unitarity does not follow, contrary to early claims, eigenvalues of the monodromy matrix not being pure phases. In the attractive regime, we find that the proposed minimal solution of the bootstrap equations is actually far from being complete. We discuss some simple values of the couplings, where, instead of the few conjectured breathers, a very complex structure (involving E_6, or two E_8) of bound states is necessary to close the bootstrap.Comment: 6 pages, 2 figures; some minor changes; accepted for publication in Phys. Lett.

Reconstructing Deconstruction: High-Velocity Cloud Distance Through Disruption Morphology

We present Arecibo L-band Feed Array 21-cm observations of a sub-complex of HVCs at the tip of the Anti-Center Complex. These observations show morphological details that point to interaction with the ambient halo medium and differential drag within the cloud sub-complex. We develop a new technique for measuring cloud distances, which relies upon these observed morphological and kinematic characteristics, and show that it is consistent with H-alpha distances. These results are consistent with distances to HVCs and halo densities derived from models in which HVCs are formed from cooling halo gas.Comment: 8 pages, 2 figures, 1 tabe, Accepted to Ap