Transcriptome Profiling of Lotus japonicus Roots During Arbuscular Mycorrhiza Development and Comparison with that of Nodulation

To better understand the molecular responses of plants to arbuscular mycorrhizal (AM) fungi, we analyzed the differential gene expression patterns of Lotus japonicus, a model legume, with the aid of a large-scale cDNA macroarray. Experiments were carried out considering the effects of contaminating microorganisms in the soil inoculants. When the colonization by AM fungi, i.e. Glomus mosseae and Gigaspora margarita, was well established, four cysteine protease genes were induced. In situ hybridization revealed that these cysteine protease genes were specifically expressed in arbuscule-containing inner cortical cells of AM roots. On the other hand, phenylpropanoid biosynthesis-related genes for phenylalanine ammonia-lyase (PAL), chalcone synthase, etc. were repressed in the later stage, although they were moderately up-regulated on the initial association with the AM fungus. Real-time RT–PCR experiments supported the array experiments. To further confirm the characteristic expression, a PAL promoter was fused with a reporter gene and introduced into L. japonicus, and then the transformants were grown with a commercial inoculum of G. mosseae. The reporter activity was augmented throughout the roots due to the presence of contaminating microorganisms in the inoculum. Interestingly, G. mosseae only colonized where the reporter activity was low. Comparison of the transcriptome profiles of AM roots and nitrogen-fixing root nodules formed with Mesorhizobium loti indicated that the PAL genes and other phenylpropanoid biosynthesis-related genes were similarly repressed in the two organs

A plastidial sodium-dependent pyruvate transporter (vol 476, pg 472, 2011)

Furumoto T, Yamaguchi T, Ohshima-Ichie Y, et al. A plastidial sodium-dependent pyruvate transporter (vol 476, pg 472, 2011). Nature. 2011;478(7368):274

History-dependent growth and reduction of the ripples formed on a swept granular track

When a solid object or wheel is repeatedly dragged on a dry sandy surface, ripple patterns are formed. Although the conditions to form ripple patterns have been studied well, methods to eliminate the developed ripple patterns have not been understood thus far. Therefore, history-dependent stability of the ripple patterns formed on a sandy surface is investigated in this study. First, the ripple patterns are formed by sweeping the flat sandy surface with a flexible plow at a constant speed. Then, the sweeping speed is reduced, and the variation of ripple patterns is measured. As a result, we find that the ripple patterns show hysteresis. Specifically, the increase in amplitude of ripples is observed when the reduced velocity is close to the initial velocity forming the ripple pattern. In addition, splitting of ripples is found when the reduced velocity is further decreased. From a simple analysis of the plow’s motion, we discuss the physical mechanism of the ripple splitting

Regulatory phosphorylation of plant phosphoenolpyruvate carboxylase: role of a conserved basic residue upstream of the phosphorylation site

AbstractIn order to mimic regulatory phosphorylation of the Ser-15 of maize C4-form phosphoenolpyruvate carboxylase (PEPC), we replaced Ser-15 and Lys-12 with Asp (S15D) and Asn (K12N), respectively, by site-directed mutagenesis. Although both mutant enzymes were catalytically as active as the wild-type PEPC, they showed much less sensitivity to malate, an allosteric inhibitor, similarly to the phosphorylated wild-type PEPC. A maize protein kinase of 30 kDa which is known to be specific to PEPC (PEPC-PK), phosphorylated K12N as well as the wild-type PEPC but not S15D. The phosphorylation of K12N further diminished the sensitivity to malate. Thus, a positive charge of the conserved Lys-12 is not required for the recognition by PEPC-PK but contributes to the intrinsic sensitivity to malate inhibition

History-dependent growth and reduction of the ripples formed on a swept granular track

When a solid object or wheel is repeatedly dragged on a dry sandy surface, ripple patterns are formed. Although the conditions to form ripple patterns have been studied well, methods to eliminate the developed ripple patterns have not been understood thus far. Therefore, history-dependent stability of the ripple patterns formed on a sandy surface is investigated in this study. First, the ripple patterns are formed by sweeping the flat sandy surface with a flexible plow at a constant speed. Then, the sweeping speed is reduced, and the variation of ripple patterns is measured. As a result, we find that the ripple patterns show hysteresis. Specifically, the increase in amplitude of ripples is observed when the reduced velocity is close to the initial velocity forming the ripple pattern. In addition, splitting of ripples is found when the reduced velocity is further decreased. From a simple analysis of the plow’s motion, we discuss the physical mechanism of the ripple splitting

Temporary expression of the TAF10 gene and its requirement for normal development of Arabidopsis thaliana

TAF10 is one of the TATA box-binding protein (TBP)-associated factors (TAFs), which constitute a TFIID with a TBP. Initially most TAFs were thought to be necessary for accurate transcription initiation from a broad group of core promoters. However, it was recently revealed that several TAFs are expressed in limited tissues during animal embryogenesis, and are indispensable for normal development of the tissues. They are called "selective" TAFs. In plants, however, little is known as to thus "selective" TAFs and their function. Here we isolated the Arabidopsis thaliana TAF10 gene (atTAF10), which is a single gene closely related to the TAF10 genes of other organisms. atTAF10 was expressed transiently during the development of several organs such as lateral roots, rosette leaves and most floral organs. Such an expression pattern was clearly distinct from that of Arabidopsis Rpb1, which encodes a component of RNA polymerase II, suggesting that atTAF10 functions in not only general transcription but also the selective expression of a subset of genes. In a knockdown mutant of atTAF10, we observed several abnormal phenotypes involved in meristem activity and leaf development, suggesting that atTAF10 is concerned in pleiotropic, but selected morphological events in Arabidopsis. These results clearly demonstrate that TAF10 is a "selective" TAF in plants, providing a new insight into the function of TAFs in plants