Studies of English Instruction Suited for Elementary School Students\u27 Developmental Stages Using Songs and Chants: Focusing on Prosodic and Emotional Effects

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Molecular cloning of a cDNA encoding human ribosomal protein L39

A cDNA clone encoding a human ribosomal protein L39 (hRPL39) was isolated through a random cDNA sequencing approach to a cDNA library constructed from a human colon carcinoma cell line of COLO 205. Although levels of hRPL39 mRNA were different in several cell lines including carcinoma cell lines from different tissues, they were shown not to be cell cycle-dependent in a human fibroblast cell line of TIG-1

Human calgizzarin ; one colorectal cancer-related gene selected by a large scale random cDNA sequencing and Northern blot analysis

A cDNA library was constructed from COLO 205 and 1056 clones randomly selected from this library were partially sequenced. Two hundred and two (38.4%) out of 526 independent genes had more than 80% similarity to the genes reported in GenBank. In Northern blot analysis, 96 out of 98 genes were shown to be expressed at the same level in colon and lung carcinoma cell lines and control fibroblasts. Only two clones, including human synovial phospholipase A-2 and a homologue to rabbit calgizzarin, were expressed at different levels among these cell lines. The full sequence of human calgizzarin was determined and its expression was remarkably elevated in colorectal cancers compared with that in normal colorectal mucosa

Extending the Operating Distance of Inductive Proximity Sensor Using Magnetoplated Wire

Inductive proximity sensors are noncontact sensing devices used to detect the approach of a target by an increase in coil resistance due to eddy current loss. Extending the operating distance of these sensors is demanded. In this paper, we propose the use of a magnetoplated wire (MPW) as a sensing coil. The MPW is a copper wire, whose circumference is plated with a magnetic thin film. We analyze the impedance of a proximity sensor using a copper wire (COW) and MPW coils by a finite element method. The use of the MPW results in a decrease in AC resistance due to the proximity effect, an increase in inductance, and the generation of a higher flux than when the COW is used. Therefore, it is possible to increase the quality factor Q of the MPW coil. As a result, the operating distances of the MPW and COW coils are 5.0 and 3.8 mm, respectively. The operating distance of the MPW coil is 1.3-fold that of the COW coil.ArticleIEEE TRANSACTIONS ON MAGNETICS. 45(10):4463-4466 (2009)journal articl

Microstructure of Erbium Oxide Thin Film on SUS316 Substrate with Y₂O₃ or CeO₂ Buffer Layers Formed by MOCVD Method

Er2O3 has been known the best candidate material for insulating coating for liquid metal breeding blanket system. The formation of Er2O3 layer by MOCVD method can be succeeded on SUS316 substrate with CeO2 and Y2O3 buffer layers (100 nm and 500 nm) fabricated by RF sputtering, and their microstructures have been confirmed by SEM, TEM and STEM. The surface morphology of their layers was smaller granular structure than the previous study without buffer layer. According to cross sectional TEM (X-TEM) observation, Er2O3, CeO2/Y2O3 buffer, unknown layers and SUS substrate can be confirmed. CeO2 buffer layer has a granular structure, while Y2O3 has a columnar structure. Er2O3 layer formed on each buffer layer had finer structure without buffer layer. It has been also detected that each element does not exist so much in each layer by diffusion during fabrication according to STEM-EDS and HAADF imaging

Molecular Cloning and Expression Analysis of a Putative Nuclear Protein, SR-25

We cloned a full-length mouse cDNA and its human homologue encoding a novel protein designated as “SR-25.” In Northern blot analysis, SR-25 mRNA was expressed in all organs tested, and relatively abundant in testis and thymus. Deduced amino acid sequences of mouse SR-25 and human SR-25 showed 77.7% identity. SR-25 has a serine-arginine repeat (SR repeat) and two types of amino acid clusters: a serine cluster and a highly basic cluster. Based on the presence of many nuclear localizing signals and a similarity to RNA splicing proteins, SR-25 is strongly suggested to be a nuclear protein and may contribute to RNA splicing

Pervasive social deficits, but normal parturition, in oxytocin receptor-deficient mice

The oxytocin receptor (OXTR) and its ligand, oxytocin (OXT), regulate reproductive physiology (i.e., parturition and lactation) and sociosexual behaviors. To define the essential functions of OXTR, we generated mice with a null mutation in the Oxtr gene (Oxtr-/-) and compared them with OXT-deficient (Oxt-/-) mice. Oxtr-/- mice were viable and had no obvious deficits in fertility or reproductive behavior. Oxtr-/- dams exhibited normal parturition but demonstrated defects in lactation and maternal nurturing. Infant Oxtr-/- males emitted fewer ultrasonic vocalizations than wild-type littermates in response to social isolation. Adult Oxtr-/- males also showed deficits in social discrimination and elevated aggressive behavior. Ligand Oxt-/- males from Oxt-/- dams, but not from Oxt+/- dams, showed similar high levels of aggression. These data suggest a developmental role for the OXT/OXTR system in shaping adult aggressive behavior. Our studies demonstrate that OXTR plays a critical role in regulating several aspects of social behavior and may have important implications for developmental psychiatric disorders characterized by deficits in social behavior