The oral microbiota in colorectal cancer is distinctive and predictive

Background and aims: Microbiota alterations are linked with colorectal cancer (CRC) and notably higher abundance of putative oral bacteria on colonic tumours. However, it is not known if colonic mucosa-associated taxa are indeed orally derived, if such cases are a distinct subset of patients or if the oral microbiome is generally suitable for screening for CRC. Methods: We profiled the microbiota in oral swabs, colonic mucosae and stool from individuals with CRC (99 subjects), colorectal polyps (32) or controls (103). Results: Several oral taxa were differentially abundant in CRC compared with controls, for example, Streptococcus and Prevotellas pp. A classification model of oral swab microbiota distinguished individuals with CRC or polyps from controls (sensitivity: 53% (CRC)/67% (polyps); specificity: 96%). Combining the data from faecal microbiota and oral swab microbiota increased the sensitivity of this model to 76% (CRC)/88% (polyps). We detected similar bacterial networks in colonic microbiota and oral microbiota datasets comprising putative oral biofilm forming bacteria. While these taxa were more abundant in CRC, core networks between pathogenic, CRC-associated oral bacteria such as Peptostreptococcus, Parvimonas and Fusobacterium were also detected in healthy controls. High abundance of Lachnospiraceae was negatively associated with the colonisation of colonic tissue with oral-like bacterial networks suggesting a protective role for certain microbiota types against CRC, possibly by conferring colonisation resistance to CRC-associated oral taxa and possibly mediated through habitual diet. Conclusion: The heterogeneity of CRC may relate to microbiota types that either predispose or provide resistance to the disease, and profiling the oral microbiome may offer an alternative screen for detecting CRC

Effects of Coarse Woody Debris on Soil Temperature and Water Content in Two Reconstructed Soils in Reclaimed Boreal Forest

Coarse woody debris (CWD) and cover soils are used to expedite ecological processes in reclaimed boreal forests after oil sands mining. Soil water content and soil temperature are considered key factors for revegetation during mine reclamation as they impact soil surface and atmosphere interactions and plant growth. However, the effects of CWD and cover soils on soil water content and temperature are not well studied. This study assessed the impact of CWD size (large, small) and type (spruce: Picea mariana, aspen: Populus tremuloides) on soil water content and temperature in two soils constructed with forest floor-mineral mix (FMM) and peat-mineral mix (PMM)) at oil sands reclamation sites. Annual and summer precipitation showed year-to-year variability; mean air temperature did not. Soil cover type had a greater impact on moderating soil water content than CWD, with PMM having a stronger influence on water content and temperature than FMM. Adding CWD increased soil water content more in FMM than PMM, and the effect on soil temperature was mostly observed during the summer months. In PMM, spruce small CWD was associated with greater water content, whereas there was no distinct differentiation between CWD size and type in FFM. This study suggests application of CWD in FMM would be more beneficial than in PMM for reclamation

Effects of Coarse Woody Debris on Soil Temperature and Water Content in Two Reconstructed Soils in Reclaimed Boreal Forest

Coarse woody debris (CWD) and cover soils are used to expedite ecological processes in reclaimed boreal forests after oil sands mining. Soil water content and soil temperature are considered key factors for revegetation during mine reclamation as they impact soil surface and atmosphere interactions and plant growth. However, the effects of CWD and cover soils on soil water content and temperature are not well studied. This study assessed the impact of CWD size (large, small) and type (spruce: Picea mariana, aspen: Populus tremuloides) on soil water content and temperature in two soils constructed with forest floor-mineral mix (FMM) and peat-mineral mix (PMM)) at oil sands reclamation sites. Annual and summer precipitation showed year-to-year variability; mean air temperature did not. Soil cover type had a greater impact on moderating soil water content than CWD, with PMM having a stronger influence on water content and temperature than FMM. Adding CWD increased soil water content more in FMM than PMM, and the effect on soil temperature was mostly observed during the summer months. In PMM, spruce small CWD was associated with greater water content, whereas there was no distinct differentiation between CWD size and type in FFM. This study suggests application of CWD in FMM would be more beneficial than in PMM for reclamation

Alcohol and Associated Characteristics among Older Persons Living with Human Immunodeficiency Virus on Antiretroviral Therapy

BackgroundAlcohol use, and particularly unhealthy alcohol use, is associated with poor human immunodeficiency virus (HIV)-related outcomes among persons living with HIV (PLWH). Despite a rapidly growing proportion of PLWH ≥50 years, alcohol use and its associated characteristics are underdescribed in this population. The authors describe alcohol use, severity, and associated characteristics using data from a sample of PLWH ≥50 years who participated in a trial of a telephone-based intervention to improve adherence to antiretroviral therapy (ART).MethodsParticipants were recruited from acquired immunodeficiency syndrome (AIDS) service organizations in 9 states and included PLWH ≥50 years who were prescribed ART, reported suboptimal adherence at screening (missing >1.5 days of medication or taking medications 2 hours early or late on >3 days in the 30 days prior to screening), and consented to participate. The AUDIT-C (Alcohol Use Disorders Identification Test-Consumption) alcohol screen, sociodemographic characteristics, substance use, and mental health comorbidity were assessed at baseline. AUDIT-C scores were categorized into nondrinking, low-level drinking, and mild-moderate unhealthy, and severe unhealthy drinking (0, 1-3, 4-6, and 7-12, respectively). Analyses described and compared characteristics across drinking status (any/none) and across AUDIT-C categories among drinkers.ResultsAmong 447 participants, 57% reported drinking in the past year (35%, 15%, and 7% reported low-level drinking, mild-moderate unhealthy drinking, and severe unhealthy drinking, respectively). Any drinking was most common among men and those who were lesbian, gay, bisexual, or transgender (LGBT), married/partnered, had received past-year alcohol treatment, and never used injection drugs (P values all <.05). Differences in race, employment status, past-year alcohol treatment, and positive depression screening (P values all <.05) were observed across AUDIT-C categories, with African American race, less than full-time employment, past-year alcohol treatment, and positive depression screening being most common among those with the most severe unhealthy drinking.ConclusionsIn this sample of older PLWH with suboptimal ART adherence, a majority reported past-year alcohol use and 22% screened positive for unhealthy alcohol use. Any and unhealthy alcohol use were associated with demographics, depression, and substance use history. Further research is needed regarding alcohol use among older PLWH

Alcohol and Associated Characteristics Among Older Persons Living With Human Immunodeficiency Virus on Antiretroviral Therapy

BackgroundAlcohol use, and particularly unhealthy alcohol use, is associated with poor human immunodeficiency virus (HIV)-related outcomes among persons living with HIV (PLWH). Despite a rapidly growing proportion of PLWH ≥50 years, alcohol use and its associated characteristics are underdescribed in this population. The authors describe alcohol use, severity, and associated characteristics using data from a sample of PLWH ≥50 years who participated in a trial of a telephone-based intervention to improve adherence to antiretroviral therapy (ART).MethodsParticipants were recruited from acquired immunodeficiency syndrome (AIDS) service organizations in 9 states and included PLWH ≥50 years who were prescribed ART, reported suboptimal adherence at screening (missing >1.5 days of medication or taking medications 2 hours early or late on >3 days in the 30 days prior to screening), and consented to participate. The AUDIT-C (Alcohol Use Disorders Identification Test-Consumption) alcohol screen, sociodemographic characteristics, substance use, and mental health comorbidity were assessed at baseline. AUDIT-C scores were categorized into nondrinking, low-level drinking, and mild-moderate unhealthy, and severe unhealthy drinking (0, 1-3, 4-6, and 7-12, respectively). Analyses described and compared characteristics across drinking status (any/none) and across AUDIT-C categories among drinkers.ResultsAmong 447 participants, 57% reported drinking in the past year (35%, 15%, and 7% reported low-level drinking, mild-moderate unhealthy drinking, and severe unhealthy drinking, respectively). Any drinking was most common among men and those who were lesbian, gay, bisexual, or transgender (LGBT), married/partnered, had received past-year alcohol treatment, and never used injection drugs (P values all <.05). Differences in race, employment status, past-year alcohol treatment, and positive depression screening (P values all <.05) were observed across AUDIT-C categories, with African American race, less than full-time employment, past-year alcohol treatment, and positive depression screening being most common among those with the most severe unhealthy drinking.ConclusionsIn this sample of older PLWH with suboptimal ART adherence, a majority reported past-year alcohol use and 22% screened positive for unhealthy alcohol use. Any and unhealthy alcohol use were associated with demographics, depression, and substance use history. Further research is needed regarding alcohol use among older PLWH