345 research outputs found
Geomagnetic Disturbance accompanying the High Altitude Nuclear Deto-nation at Johnston Island on July 9th.1962
Further Notes on "Geomagnetic Disturbance accompanying the High Altitude Nuclear Detonation at Johnston Island on July 9th 1962."
Establishment of Mareks' Disease Lymphoblastoid Cell Lines from Chickens with B^AB^K of B Blood Groups
Effect of Machining Velocity in Nanoscale Machining Operations
The aim of this study is to investigate the generated forces and deformations of single crystal Cu with (100), (110) and (111) crystallographic orientations at nanoscale machining operation. A nanoindenter equipped with nanoscratching attachment was used for machining operations and in-situ observation of a nano scale groove. As a machining parameter, the machining velocity was varied to measure the normal and cutting forces. At a fixed machining velocity, different levels of normal and cutting forces were generated due to different crystallographic orientations of the specimens. Moreover, after machining operation percentage of elastic recovery was measured and it was found that both the elastic and plastic deformations were responsible for producing a nano scale groove within the range of machining velocities from 250-1000 nm/s
Microfluidic cell engineering on high-density microelectrode arrays for assessing structure-function relationships in living neuronal networks
Neuronal networks in dissociated culture combined with cell engineering
technology offer a pivotal platform to constructively explore the relationship
between structure and function in living neuronal networks. Here, we fabricated
defined neuronal networks possessing a modular architecture on high-density
microelectrode arrays (HD-MEAs), a state-of-the-art electrophysiological tool
for recording neural activity with high spatial and temporal resolutions. We
first established a surface coating protocol using a cell-permissive hydrogel
to stably attach polydimethylsiloxane microfluidic film on the HD-MEA. We then
recorded the spontaneous neural activity of the engineered neuronal network,
which revealed an important portrait of the engineered neuronal
network--modular architecture enhances functional complexity by reducing the
excessive neural correlation between spatially segregated modules. The results
of this study highlight the impact of HD-MEA recordings combined with cell
engineering technologies as a novel tool in neuroscience to constructively
assess the structure-function relationships in neuronal networks.Comment: 18 pages, 5 figure
Detection and identification of Leishmania species within naturally infected sand flies in the Andean areas of Ecuador by a polymerase chain reaction
The surveillance of prevalent Leishmania and sand fly species in endemic areas is important for prediction of the risk and expansion of leishmaniasis. In this study, we developed a polymerase chain reaction (PCR)-based method for detection of Leishmania minicircle DNA within individual sand flies. Using this method, we detected minicircle DNA in 6 (3.3%) of 183 sand flies, while 5 (3.5%) of 143 were positive for Leishmania promastigotes in the same areas by microscopic examination. The species were identified as Leishmania (Leishmania) mexicana by nucleotide sequencing of the cytochrome b gene. Additionally, all the Leishmania-positive sand flies were identified as Lutzomyia ayacuchensis by the restriction enzyme digestion of the PCR-amplified 18S ribosomal RNA gene fragments. Since this combined method is relatively easy and can process a large number of samples, it will be a powerful tool for the rapid identification of prevalent sand fly and Leishmania species as well as monitoring the infection rate in sand fly populations in endemic areas.Fil: Kato, Hirotomo. Yamaguchi University; JapĂłnFil: Uezato, Hiroshi. University of the Ryukyus; JapĂłnFil: Katakura, Ken. Hokkaido University; JapĂłnFil: Calvopina, Manuel. Kochi University. Kochi Medical School; JapĂłnFil: Marco, Jorge Diego. Kochi University. Kochi Medical School; JapĂłn. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Salta. Instituto de PatologĂa Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de PatologĂa Experimental; ArgentinaFil: Barroso, Paola Andrea. Kochi University. Kochi Medical School; JapĂłn. Consejo Nacional de Investigaciones CientĂficas y TĂ©cnicas. Centro CientĂfico TecnolĂłgico Conicet - Salta. Instituto de PatologĂa Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de PatologĂa Experimental; ArgentinaFil: Gomez, Eduardo. Universidad CatĂłlica de Guayaquil; EcuadorFil: Mimori, Tatsuyuki. Kumamoto University; JapĂłnFil: Korenaga, Masataka. Kochi University. Kochi Medical School; JapĂłnFil: Iwata, Hiroyuki. Yamaguchi University; JapĂłnFil: Nonaka, Shigeo. University ok the Ryukyus; JapĂłnFil: Hashiguchi, Yoshihisa. Kochi University. Kochi Medical School; JapĂł
Low-Temperature Luminescence Spectroscopy of Violet Sr-Al-O:Eu2+ Phosphor Particles
Violet SrâAlâO:Eu2+ phosphor particles were synthesized from a metalâethylenediaminetetraacetic acid (EDTA) solution of Sr, Al, Eu, and particulate alumina via spray drying and sintering in a reducing atmosphere. The crystal structures and emission properties at 85â300 K were investigated. The composition of the violet SrâAlâO:Eu2+ phosphor particles was determined from various SrâAlâO:Eu2+ phosphors by their emission properties' dependence on temperature. The highly crystalline SrAl12O19:Eu2+ emission phases were confirmed by their crystallite sizes and the activation energies for the 4f5dâ8S7/2 transition of the Eu2+ ion. These results showed that the material identification for the violet SrâAlâO:Eu2+ phosphor was accomplished by the low-temperature luminescence measurements
Characterization of a novel monoclonal antibody that senses nitric oxide-dependent activation of soluble guanylate cyclase
AbstractTwo monoclonal antibodies (mAbs) against bovine lung soluble guanylate cyclase (sGC) were prepared and characterized. mAb 3221 recognized both the α- and ÎČ-subunits of sGC and had greater binding affinity to the enzyme in the presence of NO. mAb 28131 recognized only the ÎČ-subunit and its affinity did not change with NO. Neither mAb cross-reacted with particulate GC. Cultured Purkinje cells from rats were treated with S-nitroso-N-acetylpenicillamine, an NO donor, and examined by immunocytochemical methods. The immunoreactivity associated with mAb 3221 increased with the cGMP content in a crude extract of cerebellum and the NO2 generated in the culture medium increased
Evaluation of microvascular obstruction after acute myocardial infarction with cardiac magnetic resonance imaging, 201-thallium and 99 m-technetium pyrophosphate scintigraphy
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