DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF THREE-COMPONENT IN TABLET DOSAGE FORMULATION

ABSTRACT:An accurate, simple, reproducible andsensitive method for the determination ofparacetamol, caffeine and chlorpheniraminemaleate in tablet dosage form is developed andvalidated. The separation is achieved usingHiQsilC18HS reverse-phase column (250 X 4.6mm I.D., particle size 5μm) using a mixture ofacetonitrile and water in the proportion 55:300with final pH of 2.4 adjusted with o-phosphoricacid as a mobile phase. The flow rate is 1.0mL/ min and effluents were monitored at 265nm. Total run time is less than 12 min. andretention time of paracetamol, caffeine andchlorpheniramine maleate are 6.742, 9.417, and3.683 min respectively. Validation of method isdone as per ICH guideline for accuracy,precision, linearity, specificity, and sensitivity.The linearity for paracetamol is found to be100-650 μg/mL where as for caffeine andchlorpheniramine maleate is found in the rangeof 15-100 μg/mL. Result of validation study isfound statistically significant because all thestatistical parameters were within theacceptance range (COV and S.D. <1.0 for bothaccuracy and precision). The limits of detection(LOD) values are 1.2014, 0.4587 and 0.8945and limit of quantitation (LOQ) values are0.5142, 0.4512 and 0.7845 μg/mL forparacetamol, caffeine and chlorpheniraminemaleate respectively. High percentage recoveryand low COV value revealed the reliability ofthe method for quantitative study of threedrugs in Fevril tablets as a quality-control toolfor routine quantitative determination ofparacetamol, caffeine and chlorpheniraminemaleate

Coenzyme Q10 and retinaldehyde co-loaded nanostructured lipid carriers for efficacy evaluation in wrinkles

<p>This study depicts coenzyme Q10 (CoQ10) and retinaldehyde (RAL) co-loaded nanostructured lipid carriers (NLCs); having activity on different targets of photoageing, which can overcome deficits of conventional topical dosage forms. The developed NLCs were characterised for particle size, polydispersity index and percent entrapment efficiency (î), followed by their incorporation into Carbopol^® 934 P-NF gel. <i>In vitro</i> cellular uptake and cytotoxicity assay was performed to evaluate NLCs and <i>in vivo</i> study on ultraviolet- (UV) induced wrinkle model to determine efficacy of NLCs. The developed stable, homogenous and spherical NLCs with size range of 200–230 nm and more than 80 î, showed prolonged, biphasic <i>in vitro</i> release pattern for CoQ10 and RAL. <i>Ex vivo</i> study portrayed negligible permeation through skin but appreciable penetration and distribution in skin layers. This has shown good uptake of both drugs with least cytotoxicity in cell culture studies. <i>In vivo</i> irritation study on Sprague Dawley (SD) rats and pharmacodynamic study on female Swiss albino mice proved it less irritant and efficacious. The developed NLCs thus hold promise in the efficient management of wrinkle and their reduction as indicated by the data obtained.</p

Solid lipid nanoparticles and nanostructured lipid carrier-based nanotherapeutics in treatment of psoriasis: a comparative study

<p><b>Background</b>: The present work focuses on the development of ultra-small solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) encapsulating cyclosporine and calcipotriol, further incorporated into gel, increasing their penetration through the skin.</p> <p><b>Research design and methods</b>: Developed SLN and NLC were characterized regarding particle size, zeta potential, %entrapment efficiency and dispersed into carbopol 934P-NF gel. Gel was further characterized for rheological behavior and spreadability. <i>Ex vivo</i> dermatokinetic by tape stripping method, <i>in vitro</i> efficacy on HaCaT cell lines and <i>in vivo</i> efficacy on imiquimod induced psoriatic model in mice were evaluated.</p> <p><b>Results</b>: Ultra-small (size<100 nm) particles were formed with high entrapment efficiency and spherical morphology. <i>Ex vivo</i> dermatokinetic studies revealed deeper and confined drug penetration of lipid formulation gel in epidermal layers as compared to free drug. <i>In vitro</i> study on HaCaT cell lines depicted higher uptake and high efficacy owing to decrease in cell viability for NLC. The anti-psoriatic efficacy in BALB/c mice (evaluated on basis of cytokine levels and skin morphology) highlighted potential of drug-loaded NLC significantly higher as compared to drug loaded SLN and marketed formulation Betagel.</p> <p><b>Conclusions</b>: The study demonstrated that NLC gel had higher efficacy in psoriatic management and hold promise for further exploration.</p