Aerobic Physical Activity Participation and Correlates of Participating in Muscle Strengthening Physical Activity: A Cross-Sectional Analysis

The purpose of this study was to evaluate differences in correlates of muscle-strengthening physical activity (PA) between those meeting and not meeting aerobic PA guidelines. A sample of college students (n = 392) completed a survey measuring constructs from the reasoned action approach for muscle-strengthening PA. Overall, 56% (n = 220) met the aerobic PA recommendations, and 25% (n = 99) met the muscle-strengthening PA recommendations. The mean age of participants was 19.9 years (± 1.76) (meeting aerobic PA = 20.0 years old ± 1.73; not meeting aerobic PA = 19.8 years old ± 1.79), and there were no differences between race or class standing between groups [a majority of the sample identified as white/Caucasian (83.4%; n = 327) and female (69.4%; n = 272)]. Separate linear regression models were created for college students meeting and not meeting aerobic PA guidelines. College students meeting aerobic guidelines (n = 220) reported significantly more muscle-strengthening PA (m = 2.89 days ± 2.0) than students not meeting guidelines (n = 172) (m = 2.06 days ± 1.9) (p \u3c 0.001; d = 0.42). Regression models showed that attitudes, perceived norms, and perceived behavioral control (PBC) explained a substantial amount of the variance of intentions for both groups [meeting (45.5%); not meeting (59.7%)], however PBC moderated the relationship between intentions and muscle-strengthening PA for those not meeting aerobic recommendations. Results demonstrate that there are different correlates for muscle-strengthening PA, based on participation in aerobic PA, which translates to a need for different intervention approaches and strategies for both groups

Structure of Escherichia coli AdhP (ethanol-inducible dehydrogenase) with bound NAD

The crystal structure of AdhP, a recombinantly expressed alcohol dehydrogenase from Escherichia coli K-12 (substrain MG1655), was determined to 2.01 angstroms resolution. The structure, which was solved using molecular replacement, also included the structural and catalytic zinc ions and the cofactor nicotinamide adenine dinucleotide (NAD). The crystals belonged to space group P21, with unit-cell parameters a = 68.18, b = 118.92, c = 97.87 angstroms, beta = 106.41 degrees. The final R-factor and R-free were 0.138 and 0.184, respectively. The structure of the active site of AdhP suggested a number of residues that may participate in a proton relay, and the overall structure of AdhP, including the coordination to structural and active-site zinc ions, is similar to those of other tetrameric alcohol dehydrogenase enzymes.YesThe manuscript was peer-reviewed by two anonymous reviewers