Optimization of pulping method for extraction of pulp from ripe persimmon (Diospyros kaki L.) and its stability during storage

Persimmon fruit (Diospyros kaki L.) is a highly nutritious fruit with bestowed antioxidant properties. But due to its perishable nature, it is produced for a very shorter period of time. Preservation of fruit in the form of pulp acts as a suitable method to provide availability of the fruit throughout the year. The main objective of this investigation was to develop persimmon pulp from the cold and hot pulping method and to assess the best preservation method for storage. Among six different treatments in cold pulping method (T1 to T3 x 2), treatment T2 (10 % water) and among 18 different treatment in hot pulping method (T1 to T9 x 2), treatment T5 (10 % water + 10 minutes cooking time) using fruit with peel was selected on the basis of nutritional and sensory attributes. Further, the pulp was preserved via heat and chemical methods. Among six different treatments, T5 (Pulp + pasteurization + 1000 ppm potassium metabisulphite (KMS) in glass bottles) was found to be best in the type of pulp. This treatment was able to retain the maximum amount of ascorbic acid (13.733 and 8.043 mg/100 g), ?-carotene (173 and 86 µg/100 g) and total phenols (3.093 and 2.873 mg/100 g), respectively during storage of 6 months. Hence, it can be suggested from the study that nutritionally rich persimmon pulp can be prepared with better storage stability which can be used by both small and large scale industries at a lower cost of production

A selective turn-on fluorescent chemosensor 1,1-diaminoazine for azinphos-methyl

Financiaciado para publicación en acceso aberto: Universidade de Vigo/CISUGDetection of organophosphorus pesticides (OPPs) is an important challenge in environmental chemistry, because their exposure to humans can cause severe health problems. In the current study, organic nanoparticles of (E)-(4-chlorophenyl)-1,1-diamino-2,3-diazabutadiene were developed using eco-friendly approach which was found to be in the range of 15–20 nm. These synthesized species exhibited both U.V. Visible and “turn-on” fluorescence responses in aqueous media for the selective detection of the extremely hazardous pesticide azinphos-methyl. These organic nanoparticles also exhibit a good linear relationship in the range of 1–100 μM and the limit of detection (LOD) is 7.4 µM. The selective fluorescence response was also observed in RO water, tap water and orange juice. The FT-IR and DFT studies helped in identifying the specific H-bonding interactions responsible for the selective detection of Azinphos-methylDepartment of Science and Technology, New-Delhi, India | Ref. SP/YO/2021/231

Comparative evaluation of locally delivered minocycline and metronidazole in the treatment of periodontitis

Objective: The aim of this study was to evaluate and compare the efficacy of subgingivally delivered Minocycline microspheres and 25% Metronidazole gel when used as an adjunct to scaling and root planing (SRP) in the treatment of chronic periodontitis. Materials and Methods: A randomized, controlled, single center study was conducted involving 60 sites in 20 patients suffering from moderate to advanced chronic periodontitis. Each patient contributed three sites which were randomized to three treatment groups: SRP + insertion of Minocycline microspheres at day 1 (Group A), SRP + insertion of Metronidazole gel at day 1 and at day 7 (Group B), and SRP alone (Group C). Gingival index (GI), plaque index (PI), probing pocket depth (PPD), and clinical attachment level (CAL) were recorded at day 1, 1 month, and 3 months post therapy. Results: All treatments showed significant reductions in PPD and CAL at 1 and 3 months when compared to baseline values ( P < 0.001). At 3 months, sites treated with minocycline showed an additional reduction in PPD of 0.85 ± 0.03 mm, significantly greater than SRP alone. Differences in mean PPD reduction between Group B and Group C and between Group A and Group B were not significant. At 3 months, difference in CAL gain between Group A and C was 0.50 ± 0.45, which was statistically significant and between Group B and C was 0.35 ± 0.11, which was not found to be statistically significant ( P = 0.20). Differences in relative CAL between Group A and Group B were also not found to be statistically significant ( P = 0.53). Conclusion: The results concluded that treatment with Minocycline microspheres and Metronidazole gel improve PPD and CAL in patients with periodontitis compared to SRP alone

Design, synthesis and biological evaluation of novel unsymmetrical azines as quorum sensing inhibitors

Targeting quorum sensing signals using quorum sensing inhibitors has opened new avenues for the application of known antibiotics. In this context, twenty five unsymmetrical azines were synthesised and evaluated as quorum sensing inhibitors. An efficient one-pot procedure was adopted that directly links 3-methyl-2-(methylthio)benzo[d]thiazol-3-ium salt, hydrazine hydrate and substituted aldehyde to give the designed compounds. The synthesized compounds were preliminarily tested for their potential to inhibit CviR receptor based QS signals in Chromobacterium violaceum. The bioassay screening results suggested that two compounds exhibited potent QS inhibition activity against CviR receptor, showing violacein inhibition (&#62;50%) at 200 μM. Further, the putative positive hits were checked for their potential to inhibit LasR receptor-based QS using the PlasB-gfp(ASV) biomonitor strain of Pseudomonas aeruginosa. These compounds were found to inhibit the QS-mediated GFP signals in a dose dependant manner. Two active compounds also exhibited biofilm clearance at 50 μM concentration. Docking studies were performed to examine their potential to bind to the LasR protein of Pseudomonas aeruginosa

Geometry Driven Intramolecular Oxidative Cyclization of Enamides: An Umpolung Annulation of Primary Benzamides with Acrylates for the Synthesis of 3‑Methyleneisoindolin-1-ones

A palladium-catalyzed tandem oxidative annulation of primary benzamides with acrylates via intermolecular <i>N</i>-alkenylation followed by intramolecular C-alkenylation yielded a stereoselective synthesis of (<i>E</i>)-3-methyleneisoindolin-1-ones. The study unveils, for the first time, that only <i>E</i>-enamides could undergo intramolecular oxidative cyclization under the optimized conditions to give isoindolinones. The current strategy represents an umpolung strategy when compared to the literature approaches that use benzamides

Azine-Hydrazone Tautomerism of Guanylhydrazones: Evidence for the Preference Toward the Azine Tautomer

Guanylhydrazones have been known for a long time and have wide applications in organic synthesis, medicinal chemistry, and material science; however, little attention has been paid toward their electronic and structural properties. Quantum chemical analysis on several therapeutically important guanylhydrazones indicated that all of them prefer the azine tautomeric state (by about 3–12 kcal/mol). A set of simple and conjugated azines were designed using quantum chemical methods, whose tautomeric preference toward the azine tautomer is in the range of 3–8 kcal/mol. Twenty new azines were synthesized and isolated in their neutral state. Variable temperature NMR study suggests existence of the azine tautomer even at higher temperatures with no traces of the hydrazone tautomer. The crystal structures of two representative compounds confirmed that the title compounds prefer to exist in their azine tautomeric form