The Ubiquity of Sidon Sets That Are Not I0I_0

We prove that every infinite, discrete abelian group admits a pair of $I_0$ sets whose union is not $I_0$. In particular, this implies that every such group contains a Sidon set that is not $I_{0}$

Circadian Disruption and Metabolic Disease: Findings from Animal Models

Social opportunities and work demands have caused humans to become increasingly active during the late evening hours, leading to a shift from the predominantly diurnal lifestyle of our ancestors to a more nocturnal one. This voluntarily decision to stay awake long into the evening hours leads to circadian disruption at the system, tissue, and cellular levels. These derangements are in turn associated with clinical impairments in metabolic processes and physiology. The use of animal models for circadian disruption provides an important opportunity to determine mechanisms by which disorganization in the circadian system can lead to metabolic dysfunction in response to genetic, environmental, and behavioral perturbations. Here we review recent key animal studies involving circadian disruption and discuss the possible translational implications of these studies for human health and particularly for the development of metabolic disease

A Ribosomal Protein Homolog Governs Gene Expression and Virulence in a Bacterial Pathogen

The molecular machine necessary for protein synthesis, the ribosome, is generally considered constitutively functioning and lacking any inherent regulatory capacity. Yet ribosomes are commonly heterogeneous in composition and the impact of ribosome heterogeneity on translation is not well understood. Here, we determined that changes in ribosome protein composition govern gene expression in the intracellular bacterial pathogen Francisella tularensis. F. tularensis encodes three distinct homologs for bS21, a ribosomal protein involved in translation initiation, and analysis of purified F. tularensis ribosomes revealed they are heterogeneous with respect to bS21. The loss of one homolog, bS21-2, resulted in significant changes to the cellular proteome unlinked to changes in the transcriptome. Among the reduced proteins were components of the type VI secretion system (T6SS), an essential virulence factor encoded by the Francisella Pathogenicity Island. Furthermore, loss of bS21-2 led to an intramacrophage growth defect. Although multiple bS21 homologs complemented the loss of bS21-2 with respect to T6SS protein abundance, bS21-2 was uniquely necessary for robust intramacrophage growth, suggesting bS21-2 modulates additional virulence gene(s) distinct from the T6SS. Our results indicate that ribosome composition in F. tularensis, either directly or indirectly, posttranscriptionally modulates gene expression and virulence. Our findings are consistent with a model in which bS21 homologs function as posttranscriptional regulators, allowing preferential translation of specific subsets of mRNAs, likely at the stage of translation initiation. This work also raises the possibility that bS21 in other organisms may function similarly and that ribosome heterogeneity may permit many bacteria to posttranscriptionally regulate gene expression. Importance: While bacterial ribosomes are commonly heterogeneous in composition (e.g., incorporating different homologs for a ribosomal protein), how heterogeneity impacts translation is unclear. We found that the intracellular human pathogen Francisella tularensis has heterogeneous ribosomes, incorporating one of three homologs for ribosomal protein bS21. Furthermore, one bS21 homolog posttranscriptionally governs the expression of the F. tularensis type VI secretion system, an essential virulence factor. This bS21 homolog is also uniquely important for robust intracellular growth. Our data support a model in which bS21 heterogeneity leads to modulation of translation, providing another source of posttranscriptional gene regulation. Regulation of translation by bS21, or other sources of ribosomal heterogeneity, may be a conserved mechanism to control gene expression across the bacterial phylogeny

The Past in the Present: How Innovative History-making Shapes Our Many Mountains

Kathryn Newfont, Professor of History at Mars Hill University, will reflect on how collaborations such as the Madison County “Forever Free” project extension can shape our understanding not only of the Appalachian region’s complex past, but also of its multi-faceted present. Drew Reisinger’s efforts to unearth the history of chattel slavery in the public records under his care set a new standard for the nation. From the beginning Reisinger emphasized the importance of this historical work to the present-day citizens of Buncombe County, who elect the Register of Deeds. Now, in another Appalachian innovation, educators and students with Mars Hill University’s Public History Program have extended this remarkable effort into neighboring Madison County. Newfont will reflect on the potential this sort of work has for transforming our understanding of slavery in the southern mountain region and beyond. She will consider ways this collaboration and others like it can not only extend our grasp of the Appalachian region’s “Many Mountains” past, but also shapes our understandings of its complex “Many Mountains” present