Gastrectomia parcial e vagotomia troncular anterior: alterações no metabolismo de cálcio. Estudo experimental em ratos

BACKGROUND: The calcium is not absorbed in the lack of hydrochloric acid and the osteomalacia and osteoporosis may occurs; it is well recognized in patients that had gastric resection. AIM: To evaluate the effects of the partial gastrectomy associated to anterior truncal vagotomy in the absorption and metabolism of calcium. METHODS: Eighteen adults male Wistar rats were submitted to partial gastrectomy associated to anterior truncal vagotomy (GXT, eight animals) and the sham operation (10 animals, control group). The diet consumption and the weight gains of the animals were measured three times during the week. The animals received formulated experimental diet orally (AIN-93M) by eight weeks. The serum calcium, urinary and fecal calcium, apparent absorption of the calcium, activity of the enzyme alkaline fosfatase and calcium in the bone were measured after 60 days. RESULTS: The sham operated animals showed higher diet consumption, weight gains, serum and urinary calcium, excretion of calcium in feces, apparent absorption of calcium and activity of the enzyme alkaline fosfatase (P<0,05) as compared to the animals of the gastrectomized group. However, the concentration of the bone calcium was increased in the animals of the gastrectomized group. CONCLUSION: Partial gastrectomy associated to anterior truncal vagotomy showed to be a good experimental model the study calcium metabolism, decreasing the calcium absorption, serum and urinary calcium and activity of the enzyme alkaline fosfatase. However, for alterations at bone level in rats suggests an experimental study in larger period.RACIONAL: O cálcio não é absorvido na ausência de ácido clorídrico e a osteomalácia e osteoporose podem ocorrer; este fato é bem reconhecido em pacientes que se submeteram à gastrectomias. OBJETIVO: Avaliar os efeitos da gastrectomia parcial associada à vagotomia troncular anterior na absorção e metabolismo de cálcio. MÉTODOS: Dezoito ratos Wistar machos e adultos foram submetidos à gastrectomia parcial e vagotomia troncular anterior (oito animais) e à laparotomia simples como grupo controle (10 animais). O consumo de dieta e o peso dos animais foram monitorados três vezes por semana. Os animais receberam dieta oral formulada experimentalmente (AIN-93M), durante 8 semanas. O cálcio sérico, cálcio urinário e fecal, absorção aparente de cálcio, atividade da enzima fosfatase alcalina e cálcio ósseo foram mensurados após 60 dias de observação. RESULTADOS: Os animais controle apresentaram médias estatísticas maiores (P<0.05) para o consumo de dieta, ganho de peso, cálcio sérico, cálcio urinário, excreção de cálcio nas fezes, absorção aparente de cálcio e atividade da enzima fosfatase alcalina comparados aos animais do grupo gastrectomizado. Entretanto, a concentração de cálcio ósseo foi superior no grupo de animais gastrectomizados (P<0,05) quando comparado com o grupo controle. CONCLUSÃO: A gastrectomia parcial associada à vagotomia troncular anterior mostrou ser um bom modelo experimental para estudo com o metabolismo de cálcio, ocasionando diminuição da absorção de cálcio, cálcio sérico, cálcio urinário e fosfatase alcalina. No entanto, para alterações a nível ósseo em ratos, sugere-se um estudo experimental em período maior.105109Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Ingestion Of Polydextrose Increase The Iron Absorption In Rats Submitted To Partial Gastrectomy.

To investigate whether polydextrose stimulates iron absorption in rats submitted to partial gastrectomy and sham operated. The rats were submitted to partial gastrectomy (Billroth II) or laparotomy (sham-operated control), in groups of 20 and 20 each respectively. The animals were fed with a control diet (AIN-93M) without polydextrose or a diet containing polydextrose (50g/Kg of diet) for eight weeks. They were divided into four subgroups: sham-operated and Billroth II gastrectomy and with or without polydextrose. Two animals died during the experiment. All rats submitted to gastrectomy received B-12 vitamin (intramuscular) each two weeks. The hematocrit and hemoglobin concentration were measured at the start and on day 30 and 56 after the beginning of the experimental period. At the end of the study, the blood was collected for determination of serum iron concentration. The diet with polydextrose reduced the excretion of iron. Apparent iron absorption was higher in the polydextrose fed groups than in the control group. The haematocrit and haemoglobin concentration were lower after Billroth II gastrectomy rats fed the control diet as compared to the polydextrose diet groups. Polydextrose increase iron absorption and prevents postgastrectomy anemia.25518-2

Dietary galactooligosaccharides increase calcium absorption in normal and gastrectomized rats

OBJECTIVE: To determine whether the galactooligosaccharide stimulates calcium absorption in partially gastrectomized rats. METHODS: Animals were submitted to laparotomy (sham-operated control) and partial gastrectomy (Billroth II) in groups of 20. They were fed a control diet (AIN-93M) without galacto-oligosaccharide or a diet containing (galacto-oligosaccharide - 50g/kg diet) for eight weeks. The animals were divided into four groups: sham-operated and non-gastrectomized with galactooligosaccharide, sham-operated and gastrectomized without galactooligosaccharide. On the final day of the study, whole blood was collected for determination of serum calcium concentration. RESULTS: In the group with galactooligosaccharides calcium excretion in feces was significantly lower than the group without prebiotics. The apparent absorption of calcium in gastrectomized and normal rats was higher in groups fed with galactooligosaccharide than in the control diet group. CONCLUSION: The ingestion of galactooligosaccharides prevents osteopenia in partially gastrectomized rats.OBJETIVO: Verificar se o galactooligossacarídeo estimula a absorção de cálcio em ratos Wistar parcialmente gastrectomizados. MÉTODOS: Os animais foram laparotomizados (controle sham-operado) e parcialmente gastrectomizados (Billroth II) em grupos de 20 e 20 cada, respectivamente. Eles foram alimentados com uma dieta controle (AIN-93M), sem galacto-oligossacarídeo ou uma dieta contendo (galacto-oligossacarídeo - 50g/kg de dieta) durante oito semanas. Os animais foram divididos em quatro subgrupos: sham-operados e não gastrectomizados com galacto-oligossacarídeo, sham-operados e gastrectomizados sem galacto-oligossacarídeo. No dia final do estudo, o sangue total foi coletado para determinação da concentração de cálcio sérico. RESULTADOS: Na dieta no grupo com galacto-oligossacarídeo a excreção do cálcio nas fezes foi significativamente menor do que no grupo sem prebióticos. A absorção aparente de cálcio em ratos gastrectomizados e normais foi maior nos grupos alimentados com galacto-oligossacarídeo do que no grupo com dieta controle. CONCLUSÃO: A dieta com galacto-oligossacarídeos previne a osteopenia em ratos parcialmente gastrectomizados.186191Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Efeitos da gastrectomia parcial e vagotomia troncular anterior no metabolismo de ferro de ratos

PURPOSE: To evaluate the effects of the partial gastrectomy associated to anterior truncal vagotomy in the absorption and metabolism of iron. METHODS: Eighteen adults male Wistar rats were submitted to the partial gastrectomy associated to anterior truncal vagotomy (eight animals) and the sham operation (10 animals, control group). The diet consumption and the weight gains of the animals were monitored three times during the week. The animals received supplements of vitamin B12, for the prevention of the megaloblastic anemia, and a formulated experimental diet orally and denominated AIN-93M by 8 weeks. The fecal iron, apparent absorption of the iron, haematocrit, haemoglobin and serum iron were measured after 60 days. RESULTS: The animals undergoing sham operation presented larger statistical averages (p<0.05) for the diet consumption, weight gains, apparent absorption of iron, haematocrit, haemoglobin and serum iron compared to the gastrectomized group. However, the excretion of iron in feces was larger in the gastrectomized group (+14.3%) (p<0.05) when compared to the control group. CONCLUSION: Partial gastrectomy associated to anterior truncal vagotomy showed to be a good experimental model the study of iron metabolism, decreasing the haematocrit, the haemoglobin and the iron absorption, consequently causing anemia.OBJETIVO: Avaliar os efeitos da gastrectomia parcial associada a vagotomia troncular anterior na absorção e metabolismo do ferro. MÉTODOS: Dezoito ratos Wistar machos e adultos foram submetidos a gastrectomia parcial e vagotomia troncular anterior (oito animais) e à laparotomia simples como grupo controle (10 animais). O consumo de dieta e o peso dos animais foram monitorados três vezes por semana. Os animais receberam suplementos de vitamina B12 para prevenção de anemia megaloblástica, além de dieta oral formulada experimentalmente e denominada AIN-93M, durante 8 semanas. O ferro fecal, a absorção aparente de ferro, o hematócrito a hemoglobina e o ferro sérico foram mensurados, após 60 dias de observação. RESULTADOS: Os animais controles apresentaram médias estatísticas maiores (p <0.05) para o consumo de dieta, ganho de peso, absorção aparente de ferro, hematócrito, hemoglobina e ferro sérico comparados aos animais gastrectomizados. Entretanto, a excreção de ferro nas fezes foi superior no grupo de animais gastrectomizados (+14.3%) (p<0.05) quando comparado com o grupo controle. CONCLUSÃO: A gastrectomia parcial associada a vagotomia troncular anterior mostrou ser um bom modelo experimental para o estudo do metabolismo do ferro, ocasionando diminuição do hematócrito, da hemoglobina e absorção de ferro, e conseqüentemente causando anemia.40140

Effects Of The Partial Gastrectomy Associated To Anterior Truncal Vagotomy In The Metabolism Of The Iron In Rats.

To evaluate the effects of the partial gastrectomy associated to anterior truncal vagotomy in the absorption and metabolism of iron. Eighteen adults male Wistar rats were submitted to the partial gastrectomy associated to anterior truncal vagotomy (eight animals) and the sham operation (10 animals, control group). The diet consumption and the weight gains of the animals were monitored three times during the week. The animals received supplements of vitamin B12, for the prevention of the megaloblastic anemia, and a formulated experimental diet orally and denominated AIN-93M by 8 weeks. The fecal iron, apparent absorption of the iron, haematocrit, haemoglobin and serum iron were measured after 60 days. The animals undergoing sham operation presented larger statistical averages (p<0.05) for the diet consumption, weight gains, apparent absorption of iron, haematocrit, haemoglobin and serum iron compared to the gastrectomized group. However, the excretion of iron in feces was larger in the gastrectomized group (+14.3%) (p<0.05) when compared to the control group. Partial gastrectomy associated to anterior truncal vagotomy showed to be a good experimental model the study of iron metabolism, decreasing the haematocrit, the haemoglobin and the iron absorption, consequently causing anemia.23401-