4 research outputs found
Correction to Structure Elucidation and Antimalarial Activity of Apicidin F: An Apicidin-like Compound Produced by Fusarium fujikuroi
Structure Elucidation and Antimalarial Activity of Apicidin F: An Apicidin-like Compound Produced by <i>Fusarium fujikuroi</i>
Apicidins are cyclic tetrapeptides
with histone deacetylase inhibitory activity. Since their discovery
in 1996 a multitude of studies concerning the activity against protozoa
and certain cancer cell lines of natural and synthetic apicidin analogues
have been published. Until now, the only published natural sources
of apicidin are the fungus <i>Fusarium pallidoroseum</i>,
later known as <i>F. semitectum</i> and two unspecified <i>Fusarium</i> strains. The biosynthetic origin
of apicidins could be associated with a gene cluster, and a biosynthetic
pathway has been proposed. Recently, our group was able to identify
for the first time an apicidin-like gene cluster in <i>F. fujikuroi</i> that apparently does not lead to the production of any known apicidin
analogue. By overexpressing the pathway-specific transcription factor
we were able to identify a new apicidin-like compound. The present
study provides the complete structure elucidation of the new compound,
named apicidin F. Activity evaluation against <i>Plasmodium falciparum</i> showed good in vitro activity with an IC<sub>50</sub> value of 0.67
μM
Isolation and Structure Elucidation of Fujikurins A–D: Products of the PKS19 Gene Cluster in <i>Fusarium fujikuroi</i>
<i>Fusarium fujikuroi</i> is a member of the <i>Gibberella fujikuroi</i> species
complex and well known for
the production of gibberellins and mycotoxins including fusarins and
fusaric acid. A recent genome sequencing study revealed that the fungus
has the genetic potential to produce many more secondary metabolites
than have been reported. This paper describes the structure elucidation
of the products of the cryptic and silent PKS19 gene cluster that
were recently identified (fujikurins A–D). We present the complete
NMR data for the structure elucidation of the main compound fujikurin
D, which shows tautomeric 1,3-diketo elements. The different tautomeric
structures could be confirmed using quantum chemical calculations.
Additionally, the structures of the minor compounds fujikurins A–C
were elucidated by high-resolution mass spectrometric fragmentation
experiments. It emerged that fujikurin A was identical to the bioactive
compound CR377 of the taxonomically unclassified <i>Fusarium</i> strain CR377, while fujikurins B–D have not been reported
from other fungi
Structure elucidation and antimalarial activity of apicidin F : an apicidin-like compound produced by Fusarium fujikuroi
Apicidins are cyclic tetrapeptides with histone deacetylase inhibitory activity. Since their discovery in 1996 a multitude of studies concerning the activity against protozoa and certain cancer cell lines of natural and synthetic apicidin analogues have been published. Until now, the only published natural sources of apicidin are the fungus Fusarium pallidoroseum, later known as F. semitectum and two unspecified Fusarium strains. The biosynthetic origin of apicidins could be associated with a gene cluster, and a biosynthetic pathway has been proposed. Recently, our group was able to identify for the first time an apicidin-like gene cluster in F. fujikuroi that apparently does not lead to the production of any known apicidin analogue. By overexpressing the pathway-specific transcription factor we were able to identify a new apicidin-like compound. The present study provides the complete structure elucidation of the new compound, named apicidin F. Activity evaluation against Plasmodium falciparum showed good in vitro activity with an IC50 value of 0.67 μM