5 research outputs found
A genome-wide function of THSC/TREX-2 at active genes prevents transcription–replication collisions
The THSC/TREX-2 complex of Saccharomyces cerevisiae
mediates the anchoring of transcribed genes
to the nuclear pore, linking transcription elongation
with mRNA export and genome stability, as shown
for specific reporters. However, it is still unknown
whether the function of TREX-2 is global and the reason
for its relevant role in genome integrity. Here, by
studying two TREX-2 representative subunits, Thp1
and Sac3, we show that TREX-2 has a genome-wide
role in gene expression. Both proteins show similar
distributions along the genome, with a gradient disposition
at active genes that increases towards the
3 end. Thp1 and Sac3 have a relevant impact on the
expression of long, G+C-rich and highly transcribed
genes. Interestingly, replication impairment detected
by the genome-wide accumulation of the replicative
Rrm3 helicase is increased preferentially at highly
expressed genes in the thp1Δ and sac3Δ mutants
analyzed. Therefore, our work provides evidence of a
function of TREX-2 at the genome-wide level and suggests
a role for TREX-2 in preventing transcription–
replication conflicts, as a source of genome instability
derived from a defective messenger ribonucleoprotein
particle (mRNP) biogenesis.Spanish Ministry of Economy and Competitiveness
[BFU2010-16372]; Junta de AndalucĂa [CVI4567 and
P12/BIO-1238]; European Union (FEDER); and a JAE
predoctoral training grant from the Spanish Research
Council (CSIC) [to J.M.S-P.]. Funding for open access
charge: Spanish Ministry of Economy and Competitiveness
[BFU2010-16372].Peer reviewe