Reconstructed <i>Carassius</i> phylogeny of the mitochondrial cyt <i>b</i> and nuclear S7 sequences.

<p>Topologies of phylogenetic trees follow BAY analysis graphical outline. Numbers at the nodes represent statistical support for BAY and MP analyses, respectively. Bootstrap supports below 50 and Bayesian posterior probabilities below 0.75 are not shown. Sequences of the analysed individuals: hybrid female (CCAHe1Fi) and male (CCAHe2Fi) are highlighted by the grey and white rectangles, respectively.</p

List of <i>Carassius</i> samples included in molecular phylogenetic analyses.

<p>List of <i>Carassius</i> samples included in molecular phylogenetic analyses.</p

28S rDNA FISH.

<p>(A, C) Chromosomes counterstained by DAPI; B&W. 28S rDNA probe showing four signals; red, both on (B) CCAHe1Fi female and (D) CCAHe2Fi male. 28S rDNA FISH signals are indicated by arrows. Scale bars = 10 μm.</p

Karyotype of the CCAHe2Fi male with 100 chromosomes with 18 5S rDNA signals.

<p>Two strong on <i>sm</i>, two weak on <i>sm</i> and 14 signals on <i>st-a</i> chromosomes, DAPI (blue) and FITC filter (green). All nine chromosome pairs are highlighted by asterisks. Scale bar = 10 μm.</p

Morphologically indistinguishable hybrid <i>Carassius</i> female with 156 chromosomes: A threat for the threatened crucian carp, <i>C</i>. <i>carassius</i>, L - Fig 8

<p>Alternative hypothetical scenarios for the origin of a triploid hybrid female with 156 chromosomes, together with the genomes of <i>C</i>. <i>gibelio</i> (A) and <i>C</i>. <i>carassius</i> (B). Scenario A) Genome addition hypothesis. Scenario B) Spontaneous allotriploid origin. Capital letters “A” and “B” denote somatic genome; lowercase letters “a” and “b” show gamete chromosome complement (both egg and spermatozoa). Asterisk marks unusual number of chromosomes in the egg. 56/106 chromosomes instead of 50/100 in female gametes, respectively, might be caused by unequal chromosome segregation during meiosis II.</p

Morphological characteristics of a triploid, diploid representative and <i>C</i>. <i>carassius</i>.

<p>Morphological characteristics of a triploid, diploid representative and <i>C</i>. <i>carassius</i>.</p

Experiment 1 (glochidia infections)

Raw data from Experiment 1 (glochidia infections), see Readme file for detail

Experiment 3b (impacts on Baltic fish)

experimental data on population impacts of A. woodiana invasion for Baltic populations of R. amaru

Experiment 3a (impact on Danubian fish)

experimental data on population impacts of A. woodiana invasion for Danubian populations of R. amaru