Roadways Diagnostics by Georadar

Import 04/02/2014Práce vystihuje základní vlastnosti georadaru, provozní nastavení parametrů před započetím terénního měření s ohledem na možná omezení a aplikace georadaru. Práce je zakončena vyhodnocením vlastních výsledků měření na zkušebním úseku v Tišnově a úseku ve Starém Městě u Uherského Hradiště.This thesis describes the basic characteristics of the Ground Penetrating Radar (GPR), operating setting of parameters prior to the commencement of ground measurements with regard to possible limitations and applications of GPR. The work ends with the evaluation of my own measurements on a test section in Tišnov and a section in Staré Město u Uherského Hradiště.Prezenční227 - Katedra dopravního stavitelstvívýborn

Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3K/Akt and MAPK/Erk Activity in Mouse Embryonic Stem Cells

Reactive oxygen species (ROS) are important regulators of cellular functions. In embryonic stem cells, ROS are suggested to influence differentiation status. Regulated ROS formation is catalyzed primarily by NADPH-dependent oxidases (NOXs). Apocynin and diphenyleneiodonium are frequently used inhibitors of NOXs; however, both exhibit uncharacterized effects not related to NOXs inhibition. Interestingly, in our model of mouse embryonic stem cells we demonstrate low expression of NOXs. Therefore we aimed to clarify potential side effects of these drugs. Both apocynin and diphenyleneiodonium impaired proliferation of cells. Surprisingly, we observed prooxidant activity of these drugs determined by hydroethidine. Further, we revealed that apocynin inhibits PI3K/Akt pathway with its downstream transcriptional factor Nanog. Opposite to this, apocynin augmented activity of canonical Wnt signaling. On the contrary, diphenyleneiodonium activated both PI3K/Akt and Erk signaling pathways without affecting Wnt. Our data indicates limits and possible unexpected interactions of NOXs inhibitors with intracellular signaling pathways

Hypoxia Downregulates MAPK/ERK but Not STAT3 Signaling in ROS-Dependent and HIF-1-Independent Manners in Mouse Embryonic Stem Cells

Hypoxia is involved in the regulation of stem cell fate, and hypoxia-inducible factor 1 (HIF-1) is the master regulator of hypoxic response. Here, we focus on the effect of hypoxia on intracellular signaling pathways responsible for mouse embryonic stem (ES) cell maintenance. We employed wild-type and HIF-1α-deficient ES cells to investigate hypoxic response in the ERK, Akt, and STAT3 pathways. Cultivation in 1% O2 for 24 h resulted in the strong dephosphorylation of ERK and its upstream kinases and to a lesser extent of Akt in an HIF-1-independent manner, while STAT3 phosphorylation remained unaffected. Downregulation of ERK could not be mimicked either by pharmacologically induced hypoxia or by the overexpression. Dual-specificity phosphatases (DUSP) 1, 5, and 6 are hypoxia-sensitive MAPK-specific phosphatases involved in ERK downregulation, and protein phosphatase 2A (PP2A) regulates both ERK and Akt. However, combining multiple approaches, we revealed the limited significance of DUSPs and PP2A in the hypoxia-mediated attenuation of ERK signaling. Interestingly, we observed a decreased reactive oxygen species (ROS) level in hypoxia and a similar phosphorylation pattern for ERK when the cells were supplemented with glutathione. Therefore, we suggest a potential role for the ROS-dependent attenuation of ERK signaling in hypoxia, without the involvement of HIF-1

Behavioral and Pharmacokinetic Profile of Indole-Derived Synthetic Cannabinoids JWH-073 and JWH-210 as Compared to the Phytocannabinoid Δ9-THC in Rats

Synthetic cannabinoid compounds are marketed as “legal” marijuana substitutes, even though little is known about their behavioral effects in relation to their pharmacokinetic profiles. Therefore, in the present study we assessed the behavioral effects of systemic treatment with the two synthetic cannabinoids JWH-073 and JWH-210 and the phytocannabinoid Δ9-THC on locomotor activity, anxiety-like phenotype (in the open field) and sensorimotor gating (measured as prepulse inhibition of the acoustic startle response, PPI), in relation to cannabinoid serum levels. Wistar rats were injected subcutaneously (sc.) with JWH-073 (0.1, 0.5, or 5 mg/kg), JWH-210 (0.1, 0.5, or 5 mg/kg), Δ9-THC (1 or 3 mg/kg) or vehicle (oleum helanti) in a volume of 0.5 ml/kg and tested in the open field and PPI. Although JWH-073, JWH-210, Δ9-THC (and its metabolites) were confirmed in serum, effects on sensorimotor gating were absent, and locomotor activity was only partially affected. Δ9-THC (3 mg/kg) elicited an anxiolytic-like effect as suggested by the increased time spent in the center of the open field (p < 0.05). Our results further support the potential anxiolytic-like effect of pharmacological modulation of the endocannabinoid system

Analýza úvěrové politiky Komerční banky, akciové společnosti

Prezenční výpůjčkaVŠB - Technická univerzita Ostrava. Ekonomická fakulta. Katedra (154) financ

Vliv termínu množení na efektivitu množení dřevin bylinnými řízky

This thesis evaluates the influence of the term of propagation on the propagation efficiency of woody plants by herbaceous cuttings of these species: Berberis thunbergii DC. 'Atropurpurea Nana', Salix integra Thunb. 'Hakuro-Nishiki', Chaenomeles superba L. 'Elly Mossel', Ribes sanguineum 'King Edward VII', Potentilla fruticosa L. ' Super Red', Cornus alba L. 'Sibirica Variegata'. Experiment was carried out in Pasič spol. s.r.o. Dolní Životice company in terms of 17. 6. 2015, 1. 7. 2015 and 15. 7. 2015. Each variant had 4 repetitions with 140 pieces from each plant species. Experiment was evaluated after 55 days from cutting. It was shown that herbaceous cuttings rooting is the most influenced by the course of temperature and humidity in the room for propagation, especially by its sharp fluctuations during growth season. The most effective term for propagation of Berberis thunbergii DC. 'Atropurpurea Nana', Potentilla fruticosa L. ' Super Red', Salix integra Thunb. 'Hakuro-Nishiki' was the first term of 17. 6. 2015. For plants of Cornus alba L. 'Sibirica Variegata', Chaenomeles superba L. 'Elly Mossel', Ribes sanguineum 'King Edward VII', the third term of 15.7.2015 was the most effective. For Potentilla fruticosa L. ' Super Red', the effect of the term on maturity of cuttings is essential

Reconstruction of the Prokopa Velikého Street in Ostrava-Vítkovice

Import 03/08/2012V bakalářské práci je proveden návrh stavebních úprav, pomocí nichž dojde ke zvýšení bezpečnosti všech účastníků silničního provozu na ulici Prokopa Velikého. Dojde zde také ke zkvalitnění organizace ve statické dopravě. Pro lepší představivost práce obsahuje obrázky a výkresy s využitím informačních technologií.The bachelor work describes a proposition of construction modification of the Prokopa Velikého Street. The modifications will bring a safety increase for all of road users. Also the static traffic will be improved. Throughout this work visual aids have been employed to highlight certain points. These where produced with the computer aided design programs.Prezenční227 - Katedra dopravního stavitelstvívýborn

Processing of Selected Topics in Mathematics for Children with Limited Mobility Hands - The Second Class of Elementary School

My thesis is focused mainly on my own electronic work sheets for children with limited hand mobility - the second class of elementary school. The introduction deals with methodology of chosen topics which I have used in my work sheets. I compare teaching methods which I have used with alternative ones. The main part of my thesis aims at SMART Board 10 software for interactive whiteboard which I have used for creating my work sheets, secondly on its use, thirdly on its tools from the tool kit and at last but not at least on description of my work sheets. I also describe the teaching of a physically disabled girl with limited hand mobility that I have taught. The conclusion consists of summarizing my experience with teaching based on my work sheets and assessing my teaching lessons

Alkaline Phosphatase in Stem Cells

Alkaline phosphatase is an enzyme commonly expressed in almost all living organisms. In humans and other mammals, determinations of the expression and activity of alkaline phosphatase have frequently been used for cell determination in developmental studies and/or within clinical trials. Alkaline phosphatase also seems to be one of the key markers in the identification of pluripotent embryonic stem as well as related cells. However, alkaline phosphatases exist in some isoenzymes and isoforms, which have tissue specific expressions and functions. Here, the role of alkaline phosphatase as a stem cell marker is discussed in detail. First, we briefly summarize contemporary knowledge of mammalian alkaline phosphatases in general. Second, we focus on the known facts of its role in and potential significance for the identification of stem cells

The Mitochondrial Permeability Transition Pore—Current Knowledge of Its Structure, Function, and Regulation, and Optimized Methods for Evaluating Its Functional State

The mitochondrial permeability transition pore (MPTP) is a calcium-dependent, ion non-selective membrane pore with a wide range of functions. Although the MPTP has been studied for more than 50 years, its molecular structure remains unclear. Short-term (reversible) opening of the MPTP protects cells from oxidative damage and enables the efflux of Ca2+ ions from the mitochondrial matrix and cell signaling. However, long-term (irreversible) opening induces processes leading to cell death. Ca2+ ions, reactive oxygen species, and changes in mitochondrial membrane potential regulate pore opening. The sensitivity of the pore to Ca2+ ions changes as an organism ages, and MPTP opening plays a key role in the pathogenesis of many diseases. Most studies of the MPTP have focused on elucidating its molecular structure. However, understanding the mechanisms that will inhibit the MPTP may improve the treatment of diseases associated with its opening. To evaluate the functional state of the MPTP and its inhibitors, it is therefore necessary to use appropriate methods that provide reproducible results across laboratories. This review summarizes our current knowledge of the function and regulation of the MPTP. The latter part of the review introduces two optimized methods for evaluating the functional state of the pore under standardized conditions