HPLC elution profiles of four fecal extracts from a Bengal tiger collected on Days 89, 97, 98 and 100 (a) and from a jaguar collected on Days 70, 73, 76 and 77 (b).

<p>Immunoreactivitiy of each fraction was determined in the PGFM EIA, arrows represent the elution positions of reference standards.</p

HPLC elution profile of immunoreactive PGFM in fecal extracts of a pregnant tiger (days 97, a) and a pregnant jaguar (days 76, b).

<p>Fecal extracts of each animal were separated by RP-HPLC and then immunoreactivitiy of each fraction was determined by the PGFM EIA. Arrows indicate the elution positions of prostaglandin standards.</p

Term-wise PGFM concentration measured by EIA in Asiatic lion, Bengal tiger and jaguar.

<p>To examine differences in PGFM concentrations between different gestation periods, we divided pregnancy into three equal periods as early (1–5 weeks), mid (6–10 weeks) and late term (11–15 weeks) for Asiatic lion, Bengal tiger and jaguar.</p

Intraluteal relative mRNA levels during the breeding season in post-mating Iberian lynx.

<p>Box plots depict the distribution of data; horizontal lines indicate the median. frCL-I/II: frCL from Iberian lynx1/2; perCL-I/II: perCL from Iberian lynx 1/2 * P < 0.05, ** P < 0.005; Mann-Whitney U-test.</p

Immunohistochemical localization of CASP3 and TNF proteins in CL of the domestic cat.

<p>Active CASP3 was localized in luteal cells at the stages of: CL formation (A), development/maintenance (B), early regression (C) and late regression (D). The protein expression was consistently present throughout the CL life span, except for CA (E). TNF protein expression was identified in non-steroidogenic cells, presumably macrophages, but not in luteal cells (F). Arrows indicate positive staining. NC: negative control. Scale bar– 50 μm.</p

List of analyzed factors, sequences of PCR primers used for sequence analysis and expression studies, annealing temperatures, and product sizes.

<p>* GenBank sequences were obtained in this study</p><p>bp, base pair; fw, forward; rv, reverse; T_A, annealing temperature; a, used for sequence analysis; b, used for gene expression studies. Intrinsic/extrinsic pathway refers to the apoptosis pathways</p

Intraluteal concentrations of estrogens and progesterone in Iberian and Eurasian lynx.

<p>Intraluteal concentrations of estrogens and progesterone in the domestic cat can be found in Amelkina <i>et al</i> [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0143414#pone.0143414.ref024" target="_blank">24</a>]</p

F1T_coverage_MethLevels

MethyPipe input data sons testis with coverage, raw methylation levels. Columns: scaffold, starting position, strand, CpG, methylation level, coverag

Venn_data_genes_table_F0LF1LF1T_withGO

DMRs and GO result

Schematic lynx ovarian cycle.

<p>Schematic diagram of the development of follicles and CLs within a reproductive year in pregnant and non-pregnant lynx. P4, E2, PGFM in ng/mL are shown with lines.</p