865 research outputs found
Assessment of Oxidative Stress in Peste Des Petits Ruminants (Ovine Rinderpest) Affected Goats
The aim of the present investigation was to evaluate oxidative stress in goats affected with peste des petits ruminants (PPR). The experiment was designed to collect blood samples from PPR affected as well as healthy goats during a series of PPR outbreaks which occurred during February to April 2012 in different districts of Rajasthan state (India). Out of total 202 goats of various age groups and of both the sexes, 155 goats were PPR affected and 47 were healthy. Oxidative stress was evaluated by determining various serum biomarkers viz. vitamin A, vitamin C, vitamin E, glutathione, catalase, superoxide dismutase, glutathione reductase and xanthine oxidase, the mean values of which were 1.71±0.09 µmol L-1, 13.02±0.14 µmol L-1, 2.22±0.09 µmol L-1, 3.03±0.07 µmol L-1, 135.12±8.10 kU L-1, 289.13±8.00 kU L-1, 6.11± 0.06 kU L-1 and 98.12±3.12 mU L-1, respectively. Each parameter analysis of variance showed highly significant effect (P=0.0001) of health status and age category. Further interaction between health status and age category was also highly significant (P=0.0001) for each parameter studied. The results indicated that vitamins A, C, E and glutathione levels depressed by 18.95%, 38.67%, 47.64%, and 47.39%, respectively and the serum catalase, superoxide dismutase, glutathione reductase and xanthine oxidase activities increased by 90.79%, 75.11%, 90.34%, and 44.06%, respectively in affected animals as compared to that in healthy ones. On the basis of the altered levels of serum biomarkers of oxidative stress it was concluded that the animals affected with PPR developed oxidative stress
Evaluation of Antimicrobial Efficacy Against E. Coli Isolates of Different Serogroups Obtained From Diarrhoeic Neonates of Calves, Kids, and Lambs
Fourteen antimicrobials were evaluated their efficacy against 115 Escherichia coli isolates recovered from faecal samples collected from neonatal diarrhoeic calves (66), kids (25) and lambs (24). Of these isolates 37 were nontypeable, four were of rough type and 74 isolates were typeable for 'O' antigen belonging to 19 different serogroups (O2, O3, O5, O17, O21, O22, O25, O41, O45, O55, O60, O65, O70, O76, O114, O116, O147, O152, and O158). Evaluation of antimicrobial efficacy by disc diffusion method revealed that the most effective antibiotics were ciprofloxacin, sparfloxacin and triple sulpha followed by co-trimoxazole, chloramphenicol, nitrofurantoin, gentamicin, nalidixic acid, polymixin-B and amoxyclav in decreasing order of their efficacy while ampicillin and cephotaxime were found totally ineffective against these isolates. All the isolates showed multiple drug resistance to various antibiotics ranging from two to ten antibiotics. The resistance patterns were independent of serogroups of isolates and the animal species from which isolates were obtained. However, there was a variation in resistance pattern for same antibiotic vis-a vis locations of their isolation
Assessment of Oxidative Stress in Peste des petits ruminants (Ovine rinderpest) Affected Goats
The aim of the present investigation was to evaluate oxidative stress in goats affected with peste des petits ruminants (PPR). The experiment was designed to collect blood samples from PPR affected as well as healthy goats during a series of PPR outbreaks which occurred during February to April 2012 in different districts of Rajasthan state (India). Out of total 202 goats of various age groups and of both the sexes, 155 goats were PPR affected and 47 were healthy. Oxidative stress was evaluated by determining various serum biomarkers viz. vitamin A, vitamin C, vitamin E, glutathione, catalase, superoxide dismutase, glutathione reductase and xanthine oxidase, the mean values of which were 1.71±0.09 µmol L-1, 13.02±0.14 µmol L-1, 2.22±0.09 µmol L-1, 3.03±0.07 µmol L-1, 135.12±8.10 kU L-1, 289.13±8.00 kU L-1, 6.11± 0.06 kU L-1 and 98.12±3.12 mU L-1, respectively. Each parameter analysis of variance showed highly significant effect (P=0.0001) of health status and age category. Further interaction between health status and age category was also highly significant (P=0.0001) for each parameter studied. The results indicated that vitamins A, C, E and glutathione levels depressed by 18.95%, 38.67%, 47.64%, and 47.39%, respectively and the serum catalase, superoxide dismutase, glutathione reductase and xanthine oxidase activities increased by 90.79%, 75.11%, 90.34%, and 44.06%, respectively in affected animals as compared to that in healthy ones. On the basis of the altered levels of serum biomarkers of oxidative stress it was concluded that the animals affected with PPR developed oxidative stress
Evaluation of Oxidative Stress in Sheep Affected with Peste des petits ruminants
The aim of the investigation was to evaluate oxidative stress in sheep affected with peste des petits ruminants (PPR). Oxidative stress in the affected sheep was evaluated by determining various serum biomarkers viz. vitamin A, vitamin C, vitamin E, glutathione, catalase, superoxide dismutase, glutathione reductase and xanthine oxidase, the mean values of which were 1.70±0.07µmol L-1, 13.00± 0.10 µmol L-1, 2.25 ±0.07 µmol L-1, 3.10 ±0.06 µmol L-1, 140.00 ±8.00 kU L-1, 294.22 ±9.91 kU L-1, 6.99± 0.05 kU L-1 and 100.10 ±3.00 m U L-1, respectively. The levels of vitamins A, C, E and glutathione decreased significantly (p≤0.05) and the serum catalase, superoxide dismutase, glutathione reductase and xanthine oxidase activities increased significantly (p≤0.05) in affected sheep as compared to that in healthy ones. On the basis of the altered levels of serum biomarkers of oxidative stress it was concluded that the animals affected with PPR developed oxidative stress. The findings suggested the relevance of periodic assessment of oxidative status in ruminants for healthier management through supplementation of proper antioxidants as supportive treatment in PPR and in healthy in-contact animals
Evaluation of Antimicrobial Efficacy Against E. coli Isolates of Different Serogroups Obtained from Diarrhoeic Neonates of Calves, Kids, and Lambs
Fourteen antimicrobials were evaluated their efficacy against 115 Escherichia coli isolates recovered from faecal samples collected from neonatal diarrhoeic calves (66), kids (25) and lambs (24). Of these isolates 37 were nontypeable, four were of rough type and 74 isolates were typeable for 'O' antigen belonging to 19 different serogroups (O2, O3, O5, O17, O21, O22, O25, O41, O45, O55, O60, O65, O70, O76, O114, O116, O147, O152, and O158). Evaluation of antimicrobial efficacy by disc diffusion method revealed that the most effective antibiotics were ciprofloxacin, sparfloxacin and triple sulpha followed by co-trimoxazole, chloramphenicol, nitrofurantoin, gentamicin, nalidixic acid, polymixin-B and amoxyclav in decreasing order of their efficacy while ampicillin and cephotaxime were found totally ineffective against these isolates. All the isolates showed multiple drug resistance to various antibiotics ranging from two to ten antibiotics. The resistance patterns were independent of serogroups of isolates and the animal species from which isolates were obtained. However, there was a variation in resistance pattern for same antibiotic vis-a vis locations of their isolation
Broken time-reversal symmetry in cubic skutterudite-like superconductor YRuGe
The microscopic properties of superconducting cubic skutterudite-like
material YRuGe are investigated using muon spin relaxation and
rotation (SR) measurements. Zero-field SR measurements reveal the
presence of a spontaneous internal field with a magnitude of 0.18~mT
below the superconducting transition temperature, indicating broken
time-reversal symmetry in the ground state. In line with previous experiments,
transverse-field SR measurements are consistent with a fully developed
superconductivity gap in YRuGe. Our observations point towards
the relevance of electronic correlations beyond electron-phonon coupling as
origin and indicate that spin-orbit coupling is likely not the key driving
force behind the spontaneous breaking of time-reversal symmetry in this system.Comment: 7 pages, 3 figure
Time-reversal symmetry breaking in superconducting low-carrier-density quasi-skutterudite Lu3Os4Ge13
The complex structure of the Remeika phases, the intriguing quantum states
they display, and their low carrier concentrations are a strong motivation to
study the nature of their superconducting phases. In this work, the microscopic
properties of the superconducting phase of single-crystalline
LuOsGe are investigated by muon-spin relaxation and rotation
(SR) measurements. The zero-field SR data reveal the presence of
spontaneous static or quasi-static magnetic fields in the superconducting
state, breaking time-reversal symmetry; the associated internal magnetic field
scale is found to be exceptionally large ( 0.18~mT). Furthermore,
transverse-field SR measurements in the vortex state of
LuOsGe imply a complex gap function with significantly different
strengths on different parts of the Fermi surface. While our measurements do
not completely determine the order parameter, they strongly indicate that
electron-electron interactions are essential to stabilizing pairing in the
system, thus, demonstrating its unconventional nature.Comment: 7 pages, 2 figure
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