The assessment of influence of different nitrogen compounds and time on germination of Lupinus angustifolius seeds and chemical composition of final products

The aim of the research was to determine changes in the chemical composition in lupin seeds during seed germination in a solution containing selected nitrogen compounds. Blue lupin seeds of the Neptun cultivar were germinated for 3 or 4 days in darkness, at 24°C in 1% solutions containing the following nitrogen compounds: urea, nitric acid, ammonium sulphate, methionine or yeast extract. The control consisted of seeds germinated in water. The presence of all nitrogen sources has resulted in a poorer growth of the sprouts as compared to seeds germinated in water. There was a significant increase in the true protein content as well as a significant decrease in the content of crude ash and alkaloids in the germinated seeds. The seeds germinated for 4 days had significantly higher total protein content and significantly lower content of nitrogen-free extract compounds and oligosaccharides as compared to the seeds germinated for 3 days. A significant influence of the nitrogen source on the concentration of some amino acids was found. Cystine was found to be absent in seeds germinated in the nitric acid solution, while there occurred a twofold increase in the cystine content and a fivefold increase in the methionine content in seeds germinated in the aqueous solution of methionine and an 50% increase in the cystine concentration was observed in seeds germinated in the yeast extract and ammonium sulphate solution. Methionine and cystine were the limiting amino acids in all the samples, accept of seeds germinated in the methionine solution were it was valine. Aqueous solutions of ammonium sulphate and yeast extract were found to have the most advantageous influence on the chemical composition of lupin germination products

Učinak fermentacije pomoću suhog kvasca na kemijski sastav i svojstva proteina u sjemenkama plave lupine

The eff ect of 24-hour fermentation of lupin seeds by different yeast strains on their chemical composition was determined. After fermentation, the mass fraction of proteins increased and their in vitro digestibility and biological activity significantly improved. The amino acid profi le of fermented products was similar to that of raw lupin seeds. The significant reduction in the mass fraction of oligosaccharides and phytate, but not of alkaloids was found. The pH level of fermented products decreased as a consequence of the increase of lactic and propionic acid mass fractions. The most favourable changes in the Chemical composition of blue lupin seeds were obtained in fermentation with Saccharomyces cerevisiae baker’s yeast and Fermivin 7013 strain.Određen je učinak 24-satne fermentacije sjemenki lupine pomoću različitih sojeva kvasca na njihov kemijski sastav. Nakon fermentacije se bitno povećao maseni udjel proteina, njihova probavljivost in vitro i biološka aktivnost. Aminokiselinski profil fermentiranih proizvoda bio je sličan onom sirovih sjemenki lupine. Pronađen je bitno manji maseni udjel oligosaharida i fitata, ali ne i alkaloida. Povećali su se udjeli mliječne i propionske kiseline, što je bitno smanjilo pH-vrijednost fermentiranih proizvoda. Najbolji kemijski sastav imale su sjemenke plave lupine fermentirane pomoću pekarskog kvasca (Saccharomyces cerevisiae) i soja Fermivin 701

Hranjiva vrijednost i fiziološki učinak hrane za štakore s dodatkom sirovih sjemenki lupine te sjemenki fermentiranih s pomoću kvasca Candida utilis

The growth and physiological responses of the rats to diet supplemented with raw and Candida utilis-fermented yellow and blue lupin seeds were determined. The diets containing soya bean meal, raw and fermented blue and yellow lupin were administered to eight rats in each diet group for four weeks. Yellow lupin seeds in the diets of rats improved significantly (p<0.05) feed intake, protein digestibility, body mass gain and protein efficiency ratio in comparison with blue lupin seeds. On the contrary, blue lupin seeds affected significantly (p<0.05) gastrointestinal fermentation processes in comparison with yellow lupin seeds. Fermentation of lupin seeds increased crude protein content and reduced phytate and oligosaccharide content. In the fermented products, a higher number of lactic acid bacteria and yeasts but reduced number of coliform bacteria was found. Fermentation by Candida utilis positively (p<0.05) aff ected protein digestibility of feed, body mass gain and protein efficiency ratio of rats, as well as the activity of some bacterial enzymes and cholesterol concentrations in the blood serum.U radu je ispitan utjecaj hranidbe štakora sjemenkama žute i plave lupine, sirovim ili fermentiranim s pomoću kvasca Candida utilis, na njihov rast i fiziološke promjene. Štakori su u grupama po osam jedinki hranjeni sojinom sačmom, te sirovim ili fermentiranim sjemenkama plave i žute lupine tijekom četiri tjedna. Sjemenke žute lupine znatno su povećale (p<0,05) unos hrane, probavljivost proteina i tjelesnu masu, te povećale omjer proteinske djelotvornosti u usporedbi sa sjemenkama plave lupine. Sjemenke su plave lupine pak bitno utjecale (p<0,05) na fermentacijske procese u gastrointestinalnom traktu, za razliku od žute lupine. Fermentacijom se u sjemenkama lupine povećao udjel sirovih proteina, a smanjio udjel fitata i oligosaharida. U fermentiranim je proizvodima pronađeno više bakterija mliječno-kiselog vrenja te kvasaca, a manje koliformnih bakterija. Fermentacija s pomoću kvasca Candida utilis pozitivno (p<0,05) je utjecala na probavljivost proteina, povećanje tjelesne mase i omjer proteinske djelotvornosti, koncentraciju kolesterola u krvi, te na aktivnost nekih bakterijskih enzima

A comparison of biochemical and genetic classification of Propionibacterium acnes strains isolated from skin lesions of patients with acne

Przeprowadzono analizę 66 szczepów Propionibacterium acnes. Szczepy podzielono na typy biochemiczne i genetyczne; oceniano także występowa­nie β-hemolizy. Biochemiczny typ I sorbitolo-dodatni dominował u 79%, a hemolizę typu β zaobserwowano u 36% badanych szczepów. Wszystkie szczepy typu biochemicznego I reprezentowały genotyp A. Typ II charak­teryzował się wysoką zmiennością genotypową obejmując genotypy A, A’, B i C. Uzyskane wyniki uzupełnią wiedzę o różnorodności biochemicznej i genetycznej P. acnes

Feasibility of FPGA to HPC computation migration of plasma impurities diagnostic algorithms

We present a feasibility study of fast events parameters estimation algorithms regarding their execution time. It is the first stage of procedure used on data gathered from gas electron multiplier (GEM) detector for diagnostic of plasma impurities. Measured execution times are estimates of achievable times for future and more complex algorithms. The work covers usage of Intel Xeon and Intel Xeon Phi - high-performance computing (HPC) devices as a possible replacement for FPGA with highlighted advantages and disadvantages. Results show that less than 10 ms feedback loop can be obtained with the usage of 25% hardware resources in Intel Xeon or 10% resources in Intel Xeon Phi which leaves space for future increase of algorithms complexity. Moreover, this work contains a simplified overview of basic problems in actual measurement systems for diagnostic of plasma impurities, and emerging trends in developed solutions

Zero-Suppression Trigger Mode for GEM detector measurement system

A novel approach to a trigger mode in the GasElectron Multiplier (GEM) detector readout system is presented.The system is already installed at WEST tokamak. The articlebriefly describes the architecture of the GEM detector and themeasurement system. Currently the system can work in twotrigger modes: Global Trigger and Local Trigger. All trigger processingblocks are parts of the Charge Signal Sequencer modulewhich is responsible for transferring data to the PC. Therefore,the article presents structure of the Sequencer with details aboutbasic blocks, theirs functionality and output data configuration.The Sequencer with the trigger algorithms is implemented inan FPGA chip from Xilinx. Global Trigger, which is a defaultmode for the system, is not efficient and has limitations due tostoring much data without any information. Local trigger whichis under tests, removes data redundancy and is constructed tosend only valid data, but the rest of the software, especially on thePC side, is still under development. Therefore authors proposethe trigger mode which combines functionality of two existingmodes. The proposed trigger, called Zero Suppression Trigger, iscompatible with the existing interfaces of the PC software, butis also capable to verify and filter incoming signals and transferonly recognized events. The results of the implementation andsimulation are presented

Soft X-ray diagnostic system upgrades and data quality monitoring features for tokamak usage

The validation of the measurements quality after on-site diagnostic system installation is necessary in order to provide reliable data and output results. This topic is often neglected or not discussed in detail regarding measurement systems. In the paper recently installed system for soft X-ray measurements is described in introduction. The system is based on multichannel GEM detector and the data is collected and sent in special format to PC unit for further postprocessing. The unique feature of the system is the ability to compute final data based on raw data only. The raw data is selected upon algorithms by FPGA units. The FPGAs are connected to the analog front-end of the system and able to register all of the signals and collect the useful data. The interface used for data streaming is PCIe Gen2 x4 for each FPGA, therefore high throughput of the system is ensured. The paper then discusses the properties of the installation environment of the system and basic functionality mode. New features are described, both in theoretical and practical approach. New modes correspond to the data quality monitoring features implemented for the system, that provide extra information to the postprocessing stage and final algorithms. In the article is described also additional mode to perform hardware simulation of signals in a tokamak-like environment using FPGAs. The summary describes the implemented features of the data quality monitoring features and additional modes of the system