Witches, pagans and historians:an extended review of Max Dashu, <i>Witches and Pagans: Women in European Folk Religion, 700-1000 </i>

Additional file 4: Table S4. DEGs associated with the HB sources but not with the behavior. A modified t-test was performed on the log-transformed transcription ratios gained from 82 hybridizations. Oligo Ids, Gene Ids, and Comments are quoted from the UIUC Honey bee oligo 13 K v1 annotation file (May 2007)

Box plots display the uncapping engagement and performance of L1 and L2 bees (shown in blue) and H1 and H2 bees (shown in red) in the mixed L/H, pure L and pure H bee groups.

<p>(A) The proportions (%) of L1 or L2 and H1 and H2 bees that engaged in the uncapping task in the different cages. (B) The counts of uncapping acts per L1 or L2 and H1 and H2 bee that engaged in the uncapping task in the different cages. The differences between L and H bees in the mixed and pure groups in (A) and (B) were compared by <i>MWU</i>-tests (* denotes <i>P</i><0.05, <i>n</i> = 12).</p

Group behavioral uncapping responses in the mixed L/H and pure L and H bee cages.

<p>(A) The number of workers that engaged in uncapping tasks per treated brood cell (boxplot; combined data from different cages). Numbers represent cumulative data from all cages. Medians were compared with the <i>Median</i>-test; * denotes <i>P</i><0.05, and ** denotes <i>P</i><0.005. (B) The number of uncapped and non-uncapped cells in a colony as a measure of colony-level performance. The expected value in mixed cages was calculated based on the additive combination of the pure L and H bee colony outcomes and the relative contributions of bees in the mixed cages. * denotes <i>P</i><0.02. We identified 105 treated brood cells at which bees engaged in uncapping behavior, which were analyzed in (A), and 108 treated brood that were actually uncapped in the different genotypic mixed groups.</p

Modules of co-regulated transcripts identified among the 943 candidate genes that exhibited altered expression in the combined condition of behavior and genotypic mixing.

<p>|<i>r^—</i>| denotes the average correlation as estimated from the mean of all pairwise correlations of transcripts within a module. B denotes the condition of behavior, and C represents the condition of genotypic mixing. Relative proportions were calculated from the entire set of pairwise correlations within the modules. The percentage of identified orthologs in <i>Drosophila melanogaster</i> was obtained from the UIUC Honey Bee oligo 13K v1 annotation file (May 2007).</p

Hygienic behavior of honeybee workers in the Low (L), High (H), and Low/High (L/H) mixed groups.

<p>(A) Schematic representation of the experimental setup of the cages. Newly emerged L and H worker bees from the two L (L1 and L2) and two H (H1 and H2) genetic hygienic behavior sources were used to establish mixed L/H, pure L and pure H bee cages. Each cage consisted of ∼500 individually marked bees. L bees are shown in blue, and H bees are shown in red. (B) An example of a worker (tag no. 89) that engaged in uncapping behavior. The hygienic behavior of ∼12-day-old workers was monitored for 12 hours in a brood comb that contained ∼33 pin-killed pupae and control brood cells.</p

Model of the indirect genetic effects on behavioral responsiveness and gene expression in the brain.

<p>Model of the indirect genetic effects on behavioral responsiveness and gene expression in the brain.</p

SNP_Info_HoneyBeeSNPAssay.xlsx

The columns in the file "SNP_Info_HoneyBeeSNPAssay.xlsx" are as follows: -lsid:local SNP ID referring to a file (amel_v3_asm_snps.dbsnps.gz) published by the Honey Bee Genome Project (http://www.hgsc.bcm.tmc.edu/project-species-i-Apis%20mellifera.hgsc?page Location=Apis%20mellifera), which is accessible via the URL: "http://www.hgsc.bcm.tmc.edu/ftp-archive/Amellifera/snp/"; -linkage group: Amel assembly version 3.0 linkage group 1~16; -Cooridnate: base position in linkage group containing polymorphism; -allele: polymorphism in format A/G, consensus base on left; -left flanking sequence: linkage group left flanking sequence (250 bases); -right flanking sequence: linkage group right flanking sequence (250 bases

SNP data, family structure and SNP positions

The zip-file contains the SNP data ( 2 files), the identification of the queens (families) and the SNP map used

Additional file 1 of On the relevance of technical variation due to building pools in microarray experiments

Matrices for EM-REML and Mixed model equations. This file shows various matrices for the experimental data sets in detail. These matrices are explained in the Materials and Methods section. (PDF 48 kb