22 research outputs found
Inhibition of inducible nitric oxide synthase reduces myocardial injury in autoimmune myocarditis rats
The Japanese Society of Pathology Guidelines on the handling of pathological tissue samples for genomic research: Standard operating procedures based on empirical analyses
Genome research using appropriately collected pathological tissue samples is expected to yield breakthroughs in the development of biomarkers and identification of therapeutic targets for diseases such as cancers. In this connection, the Japanese Society of Pathology (JSP) has developed “The JSP Guidelines on the Handling of Pathological Tissue Samples for Genomic Research” based on an abundance of data from empirical analyses of tissue samples collected and stored under various conditions. Tissue samples should be collected from appropriate sites within surgically resected specimens, without disturbing the features on which pathological diagnosis is based, while avoiding bleeding or necrotic foci. They should be collected as soon as possible after resection: at the latest within about 3 h of storage at 4°C. Preferably, snap‐frozen samples should be stored in liquid nitrogen (about −180°C) until use. When intending to use genomic DNA extracted from formalin‐fixed paraffin‐embedded tissue, 10% neutral buffered formalin should be used. Insufficient fixation and overfixation must both be avoided. We hope that pathologists, clinicians, clinical laboratory technicians and biobank operators will come to master the handling of pathological tissue samples based on the standard operating procedures in these Guidelines to yield results that will assist in the realization of genomic medicine
The lymphocyte-dendritic cell system
Antigens provoke immune responses. The
group of immunocompetent cells related directly to this
response includes T and B cells, macrophages (MO) and
dendritic cells (DCs). DCs acting as antigen-presenting
cells have been recently recognized to be important in
initiating the immune response.
B cells and follicular dendritic cells (FDCs), the
major immunocompetent cells in the B-cell dependent
area, play an important role in humoral immunity, while
T cells and interdigitating cells (IDCs), which are the
major immunocompetent cells in the T-cell dependent
(TD)-area, play an important role in cellular immunity.
The B cell-IDC interaction in the TD-area is also
essential for the B-cell response against TD-antigen.
Consequently, the lymphocyte-DC interaction is
essential in the response to antigenic stimulation and in
inducing the potent effector cells. B cell-DC, T cell-DC
and DC-B cell-T cell interactions are regulated in predetermined
sites by complex and varied mechanisms.
Much recent evidence demonstrates that DCs modulate
lymphocyte biology in its broadest aspects, including
generation, differen-tiation, proliferation, and activation.
In this review, we outline recent studies on the
generation, structure, and function of lymphatic tissues,
propose the concept of the "Lymphocyte-Dendritic Cell
System (LDS)", and finally describe the significance and
functions of this system in health and disease
Development of follicular dendritic cells: A study using short-term bone marrow cell grafting in SCID mice
To evaluate the cellular origin of follicular
dendritic cells (FDC) in lymphoid follicles (LFs), severe
combined immunodeficient (SCID) mice (H-2d) were
grafted with 5-bromo-2'-deoxyuridine (BrdU)-
incorporated bone marrow cells from CB-17 mice (H-2'9
and with non-BrdU-incorporated bone marrow cells
from C3H mice (H-2k) and Wistar rats (RTIU). This
procedure was followed by antigenic stimulation with
horseradish peroxidase and related immune complex
(mouse peroxidase anti-peroxidase) administration.
Secondary LFs in the lymph nodes and spleen of the
reconstructed SCID mice were examined morphologically
and immunocytochemically. LFs reconstructed
with CB-17 mouse bone marrow cells contained FDCs
capable of trapping and/or retaining mouse peroxidase
anti-peroxidase as immune complexes. Secondary LFs
contained BrdU-incorporated germinal center lymphocytes
but not non-lymphoid stromal cells. A cell grafting
study in SCID mice using bone marrow cells from C3H
mice and Wistar rats demonstrated that FDCs in reconstructed
LFs exhibited a marker specific for the
recipient but not for the donor. These data indicate that
functionally active FDCs occur de novo in reconstructed
LFs in SCID mice, and do not support the view that
FDCs originate from bone marrow cells in short-term
reconstructed LFs
Endogenous sex hormone and estrogen binding activity in thyroid cancer
Summary. The reactions of four anti-sex hormone
(Estrone, Estradiol, Estriol and Testosterone) antisera
were immunohistochemically examined in 109 cancerous
and 80 normal and benign thyroid tissues. Four kinds of
sex hormones were detected in the tumour cells of 61
cases (56%) of thyroid cancer and in the follicular epithelial
cells of 4 cases (5%) of normal and benign thyroid tissues.
Among the thyroid cancers, 54 female (61%) and 7 cases
in males (33%) were positive for sex hormones. Furthermore,
estrogen binding activity was screened histochemically
in 36 thyroid tissues of various types, and detected
not only in thyroid cancer (6115 cases), but in normal and
benign thyroid tissues (3121 cases) as well. It was concluded
that endogenous estradiol was located in thyroid cancers
more frequently in females than in males and that there
was estrogen binding activity in the cells of not only
thyroid cancers, but also normal and benign thyroid
tissues. This is the first report of the dernonstration of
endogenous sex hormones in thyroid cancer
Modeling and Identification of Data Center HVAC System with Super-Multipoint Temperature Sensing System
This paper investigates a heating, ventilation, and air-conditioning (HVAC) system in a data center equipped with a previously developed super-multipoint temperature sensing system. This system is expected to be a key technology for reducing the total power consumption of the HVAC system by controlling the inlet temperature distribution of the servers in real time. For this purpose, we present an overview of our fan-control system based on model predictive control. The main objective of this paper is to identify a dynamical model of temperature variations, in order to predict the future evolution of the distribution. However, the spatially high-density temperature data provided by the sensing system is not suited to the needed model accuracy, and the present modeling problem is differentiated from standard ones. We thus present a systematic scheme for the spatial density reduction of sensors by using spectral clustering and graph theory and associated techniques to acquire the dynamical model. Through simulation with real data, we finally show that the developed model achieves an accuracy of 0.58 degrees Celsius on average
IBL-like T cell lymphoma expressing monoclonal gammopathy (macroglobulinemia) in the serum
A case of IBL-like T cell lymphoma with
serum monoclonal gammopathy was reported. A 58-
year-old woman, who had suffered from heart failure.
was admitted because of asthma attack, fever and
lyrnphadenopathy. Leucopenia with a small amount of
atypical lymphocytes was detected. Serum analysis
showed monoclonal elevation of IgM-K (M-protein) and
hv~erviscositv. Urinarv Bence-Jones urotein was
diiected. Lymph &de biopsy rAealed the
disappearance of normal structure and proliferation of T
cells with pale cells which characterized IBL-like T cell
lymphoma. Immunocytochemistry revealed the pale
cells to bear T cell markers (MT-1, CD 5, CD 8 or CD 4)
and IgM-positive cell distribution. Tonsilar biopsy
showed the infiltration of atypical lymphoids and pale
cells. Bone marrow biopsy showed moderate
lymphoplasmacytoid proliferation with lymph follicles.
Clinical data and serum analysis suggested
macroglobulinemia. Additional lymph node biopsy was
performed and revealed IBL-like T cell lymphoma. IBLlike
T cell lymphoma is characterized by polyclonal
hypergammaglobulinemia. The present case probably
occurred initially as IBL-like T cell lymphoma and
lymphoplasmacytoid cell proliferation might have
followed due to an excess of CD 4' cell
Function of the follicular dendritic cell in the germinal center of lymphoid follicles
The authors made an immunocytochemical
examination of the germinal centers (GCs) of (1) lymph
follicles in physiological lymph nodes and (2) extra-nodal
tissues of divergent diseases including thyroid disorders,
rheumatoid arthritis, Warthin's tumor and Kimura's disease
(Eosinophilic lymphfolliculoid granuloma). In these
germinal centers, the presence of immunoglobulins (IgG
and IgM), early acting complement components (Clq, C4,
C3c,C3d), receptors for C3b and C3d and dendritic
reticulum cell-l was demonstrated in lace-like network
patterns which were proven electron-microscopically to
coincide with the surface of follicular dendritic cells. IgE
was distributed in a lace-like pattern in the GCs of
proliferating follicles of Kimura's disease, in which the lysis
of follicles was frequently observed. This lysis appeared to
be related to the presence of complement components. In
the germinal centers of extra-nodal tissues, including the
thyroid tissues accompanying the lymph follicles,
rheumatoid arthritis synovial tissues as well as Warthin's
tumors, thyroglobulin, rheumatoid factor and salivary
amylase were detected as specific antigens, occurring in lacelike
patterns.
It is possible that follicular dendritic cells may play a
role in the genesis of GCs and be responsible for the
immune response through C3 receptors