Perceptions of pastoralist problems: A participatory study on animal management, disease spectrum and animal health priorities of small ruminant pastoralists in Georgia

Small ruminants support the livelihoods of millions of poor pastoralist and sedentary households around the world. While pastoralists are generally not amongst the poorest in terms of assets, they are frequently marginalised in terms of their access to political power, health and education. This study was undertaken among pastoralist households keeping small ruminants in four regions of the country of Georgia. Small ruminants are an important cultural, social and economic asset in Georgia and are mainly managed in a transhumant pastoralist system. Georgia suffered its first, and so far only outbreak of peste des petits ruminants (PPR) in 2016. This qualitative interview study was designed to acquire contextual understanding of local small ruminant husbandry and the livelihood situations of the participating pastoralists, and to detect historical, unreported PPR outbreaks. Focus group discussions comprising participatory epidemiology tools and other forms of interviews were used to explore small ruminant management, disease spectrum and management, and animal health priorities.The participants had experienced a wide variety of animal health constraints, with intestinal worms, braxy, piroplasmosis, pasture-related problems, predators and lameness emerging as priorities. No historic, unreported PPR outbreak was detected in this study, and PPR was not a priority for participants. Instead, the day-to-day reality of animal health for the pastoralists was characterised by co-infections of mainly endemic pathogens, and problems related to other challenges such as access to land, feed and genetic resources. The rationale behind the participants' prioritisation of animal health problems was supported by the need to pay extra attention to animals in order to avoid risk factors, keep animals healthy and minimise the negative impact of diseases or management problems; the various epidemiological and clinical parameters of the prioritised diseases; the economic impact of the specific problems and the zoonotic potential of diseases and predation. Even within regions, and within seemingly socially and culturally homogenous groups, there were important local differences in the problems faced by pastoralists that affect their livestock management. This study underlines the importance of a contextualised understanding of the local disease panorama and complexities in the livelihood situations of rural people when designing actions to improve animal health in general or, more specifically, passive surveillance as well as prevention or control measures. Finally, it is concluded that to achieve such an understanding, there is a need for participatory, scoping-style studies that specifically acknowledge diversity and power relations

Comparing the genetic typing methods for effective surveillance and rabies control in Georgia

A full nucleoprotein gene sequencing of 68 isolates collected from passive rabies surveillance system in Georgia between 2015 and 2016 identified two distinct dog rabies phylogroups, GEO_V1 and GEO_V2, which both belonged to the cosmopolitan dog clade. GEO_V1 was found throughout the country and was further divided into four sub-phylogroups that overlapped geographically; GEO_V2 was found in the southeast region and was closely related to dog rabies in Azerbaijan. A sequence analysis of the full N gene, partial nucleoprotein gene of N-terminal and C-terminal, and the amplicon sequences of pan-lyssavirus RT-qPCR LN34 showed that all four sequencing approaches provided clear genetic typing results of canine rabies and could further differentiate GEO_V1 and GEO_V2. The phylogenetic analysis results vary and were affected by the length of the sequences used. Amplicon sequencing of the LN34 assay positive samples provided a rapid and cost-effective method for rabies genetic typing, which is important for improving rabies surveillance and canine rabies eradication globally

Implementation of the One Health approach to fight arbovirus infections in the Mediterranean and Black Sea Region: Assessing integrated surveillance in Serbia, Tunisia and Georgia

Background In the Mediterranean and Black Sea Region, arbovirus infections are emerging infectious diseases. Their surveillance can benefit from one health inter-sectoral collaboration; however, no standardized methodology exists to study One Health surveillance. Methods We designed a situation analysis study to document how integration of laboratory/clinical human, animal and entomological surveillance of arboviruses was being implemented in the Region. We applied a framework designed to assess three levels of integration: policy/institutional, data collection/data analysis and dissemination. We tested the use of Business Process Modelling Notation (BPMN) to graphically present evidence of inter-sectoral integration. Results Serbia, Tunisia and Georgia participated in the study. West Nile Virus surveillance was analysed in Serbia and Tunisia, Crimea-Congo Haemorrhagic Fever surveillance in Georgia. Our framework enabled a standardized analysis of One Health surveillance integration, and BPMN was easily understandable and conducive to detailed discussions among different actors/institutions. In all countries, we observed integration across sectors and levels except in data collection and data analysis. Data collection was interoperable only in Georgia without integrated analysis. In all countries, surveillance was mainly oriented towards outbreak response, triggered by an index human case. Discussion The three surveillance systems we observed prove that integrated surveillance can be operationalized with a diverse spectrum of options. However, in all countries, the integrated use of data for early warning and inter-sectoral priority setting is pioneeristic. We also noted that early warning before human case occurrence is recurrently not operationally prioritized

Post-vaccination rabies sero-survey in Georgia, 2015

ObjectiveThe objective of this survey was to study vaccination coverage andquality in dogs in Georgia through the detection of post-vaccinationantibodies.IntroductionRabies is endemic in Georgia with up to 100 confirmed cases inanimals per year. There is an estimated 350,000 domestic and straydogs/cats in Georgia. The prophylactic vaccination of domesticanimals against rabies was reestablished in Georgia in 2013. Each yearsince 2013, coverage has increased aiming to cover approximately70% of the total population of dogs/cats in Georgia.MethodsOnly vaccinated dog populations were included in the sero-survey. Using random selection, five locations were selected. Thesurvey was conducted over a period of 4-8 weeks after vaccination.In order to study vaccination coverage, the total dog population wasregistered. Samples were taken only from vaccinated dogs (confirmedby vaccination papers) and samples were sent to the Laboratory ofthe Ministry of Agriculture where they were tested for the presenceof antibodies using ELISA. Epidemiological information and GPScoordinates were recorded in the electronic integrated diseasesurveillance system (EIDSS) and geographic information system(GIS).ResultsOut of 572 dogs in sampled villages, 373 animal’s vaccination wasconfirmed leading to 65% vaccination coverage. Out of 255 samples,241 were suitable for testing; 237 samples (98.3%) were positive forthe existence of antibodies. Antibody titer was not measured.ConclusionsBased on the results of the survey, it can be seen that vaccinationcoverage is generally not high (65%) and needs improvement.The vaccination quality (as determined through the existence ofantibodies) is good (98.3%). In further surveys, antibody titersmust be measured in order to extract more information regardingvaccination quality

Post-vaccination rabies sero-survey in Georgia, 2015

ObjectiveThe objective of this survey was to study vaccination coverage andquality in dogs in Georgia through the detection of post-vaccinationantibodies.IntroductionRabies is endemic in Georgia with up to 100 confirmed cases inanimals per year. There is an estimated 350,000 domestic and straydogs/cats in Georgia. The prophylactic vaccination of domesticanimals against rabies was reestablished in Georgia in 2013. Each yearsince 2013, coverage has increased aiming to cover approximately70% of the total population of dogs/cats in Georgia.MethodsOnly vaccinated dog populations were included in the sero-survey. Using random selection, five locations were selected. Thesurvey was conducted over a period of 4-8 weeks after vaccination.In order to study vaccination coverage, the total dog population wasregistered. Samples were taken only from vaccinated dogs (confirmedby vaccination papers) and samples were sent to the Laboratory ofthe Ministry of Agriculture where they were tested for the presenceof antibodies using ELISA. Epidemiological information and GPScoordinates were recorded in the electronic integrated diseasesurveillance system (EIDSS) and geographic information system(GIS).ResultsOut of 572 dogs in sampled villages, 373 animal’s vaccination wasconfirmed leading to 65% vaccination coverage. Out of 255 samples,241 were suitable for testing; 237 samples (98.3%) were positive forthe existence of antibodies. Antibody titer was not measured.ConclusionsBased on the results of the survey, it can be seen that vaccinationcoverage is generally not high (65%) and needs improvement.The vaccination quality (as determined through the existence ofantibodies) is good (98.3%). In further surveys, antibody titersmust be measured in order to extract more information regardingvaccination quality

Successful implementation of electronic disease reporting in Georgia

ObjectiveThe objective of this study was to evaluate several aspects of theelectronic disease reporting system and its abilities used in Georgia.Also, the study assessed if the system abilities are tailored to thenational surveillance requirements. User attitudes (system strengthand weaknesses) toward the system was also surveyed.IntroductionThe Ministry of Health of Georgia accepted the ElectronicIntegrated Disease Surveillance System (EIDSS) as an officialdisease reporting system in 2012. The Georgian government adoptedelectronic reporting for both veterinary and human diseases in2015. We conducted a comparative assessment of progress in theimplementation of electronic reporting.MethodsA face-to-face initial survey was conducted in 2012, a follow-upsurvey (through telephone interviews) was performed in 2016. Theinitial survey was conducted in regions that had EIDSS installedand the follow-up survey was conducted in all regions. Standardizedquestionnaires were used and data was analyzed in Epi Info.ResultsOut of 450 trained EIDSS users, 32% were interviewed in theinitial survey and 25% (of 550) EIDSS trained users were interviewedin the follow-up survey. Of 147 respondents in the initial survey and138 in the follow-up survey, 44% and 79%, believed that they wereusing EIDSS effectively, respectively. The follow-up survey showeda 23% increase in respondents who acknowledge an improvement ofthe electronic reporting; acceptance of EIDSS increased from 80.3%to 97.8%. Of those interviewed in the follow-up survey, 19.7%mentioned that the main success in development of the system is dueto improved collaboration between institutes. However, 17.36% of therespondents in the follow-up survey reported non-sufficient qualitydata.ConclusionsOur study suggests that the acceptance and use of EIDSS hasnoticeably improved, indicating the successful implementation ofelectronic reporting. Recommendations have been made to furtherimprove the data quality by conducting regular data cleaning andadditional user training. We recommend the continuation of EIDSStraining

Successful implementation of electronic disease reporting in Georgia

ObjectiveThe objective of this study was to evaluate several aspects of theelectronic disease reporting system and its abilities used in Georgia.Also, the study assessed if the system abilities are tailored to thenational surveillance requirements. User attitudes (system strengthand weaknesses) toward the system was also surveyed.IntroductionThe Ministry of Health of Georgia accepted the ElectronicIntegrated Disease Surveillance System (EIDSS) as an officialdisease reporting system in 2012. The Georgian government adoptedelectronic reporting for both veterinary and human diseases in2015. We conducted a comparative assessment of progress in theimplementation of electronic reporting.MethodsA face-to-face initial survey was conducted in 2012, a follow-upsurvey (through telephone interviews) was performed in 2016. Theinitial survey was conducted in regions that had EIDSS installedand the follow-up survey was conducted in all regions. Standardizedquestionnaires were used and data was analyzed in Epi Info.ResultsOut of 450 trained EIDSS users, 32% were interviewed in theinitial survey and 25% (of 550) EIDSS trained users were interviewedin the follow-up survey. Of 147 respondents in the initial survey and138 in the follow-up survey, 44% and 79%, believed that they wereusing EIDSS effectively, respectively. The follow-up survey showeda 23% increase in respondents who acknowledge an improvement ofthe electronic reporting; acceptance of EIDSS increased from 80.3%to 97.8%. Of those interviewed in the follow-up survey, 19.7%mentioned that the main success in development of the system is dueto improved collaboration between institutes. However, 17.36% of therespondents in the follow-up survey reported non-sufficient qualitydata.ConclusionsOur study suggests that the acceptance and use of EIDSS hasnoticeably improved, indicating the successful implementation ofelectronic reporting. Recommendations have been made to furtherimprove the data quality by conducting regular data cleaning andadditional user training. We recommend the continuation of EIDSStraining

Implementation of a Regional Training Program on African Swine Fever As Part of the Cooperative Biological Engagement Program across the Caucasus Region

A training and outreach program to increase public awareness of African swine fever (ASF) was implemented by Defense Threat Reduction Agency and the Ministries of Agriculture in Armenia, Georgia, Kazakhstan, and Ukraine. The implementing agency was the company SAFOSO (Switzerland). Integration of this regional effort was administered by subject matter experts for each country. The main teaching effort of this project was to develop a comprehensive regional public outreach campaign through a network of expertise and knowledge for the control and prevention of ASF in four neighboring countries that experience similar issues with this disease. Gaps in disease knowledge, legislation, and outbreak preparedness in each country were all addressed. Because ASF is a pathogen with bioterrorism potential and of great veterinary health importance that is responsible for major economic instability, the project team developed public outreach programs to train veterinarians in the partner countries to accurately and rapidly identify ASF activity and report it to international veterinary health agencies. The project implementers facilitated four regional meetings to develop this outreach program, which was later disseminated in each partner country. Partner country participants were trained as trainers to implement the outreach program in their respective countries. In this paper, we describe the development, execution, and evaluation of the ASF training and outreach program that reached more than 13,000 veterinarians, farmers, and hunters in the partner countries. Additionally, more than 120,000 booklets, flyers, leaflets, guidelines, and posters were distributed during the outreach campaign. Pre- and post-ASF knowledge exams were developed. The overall success of the project was demonstrated in that the principles of developing and conducting a public outreach program were established, and these foundational teachings can be applied within a single country or expanded regionally to disseminate disease information across borders; overall, this method can be modified to raise awareness about many other diseases