78 research outputs found
Designing Controls for Network Organisations: A Value-Based Approach
Tan, Y.H. [Promotor]Paans, R. [Promotor]Gordijn, J. [Copromotor
EFFICACY AND SAFETY OF IMMUNOMODULATORS APPLICATION IN PREVENTION OF COMPLICATIONS IN PATIENTS WITH PERTUSSIS
Pertussis is an urgent pediatric problem due to its frequency and severity in the first year of life. One of the most threatening complications of pertussis is pneumonia. In a parallel, non-randomized open-label study we have demonstrated clinical efficacy and safety of immunomodulators for the prophylaxis of bacterial complications of pertussis
Evaluation of the long-term memory T cell in mice after immunization with a live tularemia vaccine
The vaccine strain F. tularensis 15 NIIEG induces long-lived cell-mediated immunity but exhibits a certain reactogenicity and genetic instability. Progress in development of a vaccine against tularemia has been limited by a lack of information regarding the mechanisms required to protect against this disease. The BALB/c mouse is the most commonly used animal to study tularemia due to its relatively low cost, well-characterized genetics, available immunological tools and mouse infection with virulent F. tularensis recapitulates human disease.CD4+ and CD8+T cells are known to be critical for the formation of protective immunity but the relative roles of memory T cell subpopulations in long lived protection against virulent strains of F. tularensis are not well established. We hypothesized that this immunity depends on central (TCM) and effector memory (TEM) T cells and their functional activity. In this study we have dissected the T cell immune response in BALB/c mice 30, 60 and 90 days after subcutaneous vaccination with 15 NIIEG.Multiparametric flow cytometry were used to characterize in vitro recall responses of splenocytes to F. tularensis antigen. TEM cells were identified as CD3+CD4+CD44+CD62L- and CD3+CD8+CD44+CD62L-, TCM cells as CD3+CD4+CD44+CD62L+ and CD3+CD8+CD44+CD62L+, respectively. The functional activity of memory T cells was assessed by the following parameters: the level of expression of the activation marker CD69 and cytokine-producing activity by staining with the intracellular cytokines IFNg and TNFa.Thus, development of a long-lived vaccine directed against F. tularensis is dependent on identifying not only the correlates of immunity present early after vaccination, but also those that persist in the host after the effector phase has ended. The maintenance of long-term protective immunity initiated by vaccination with F. tularensis strain 15 NIIEG has been shown to require the presence of antigen-specific CD4+ and CD8+ memory T cells producing IFNg and TNFa and expressing the activation marker CD69. A decrease in count and functional activity of CD8+TCM and CD8+TEM was detected in the long term after vaccination. The detected parameters of functional activity of memory T cells can be used as criteria for evaluation of protective immunity against virulent strains of F. tularensis
ΠΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΠΈ Π±ΡΠΎΠ½Ρ ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΡ Ρ Π΄Π΅ΡΠ΅ΠΉ Π² ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ΅
Six-year dynamics of epidemiology of bronchial asthma was analyzed in Novosibirsk according to a common standardized protocol of the "International Study of Asthma and Allergic Diseases in Children" (ISAAC) programme. The pathology spread was studied using a total surveying among first-form and eight-form pupils (the survey involved 7291 pupils 1996, 6631 ones in 1999 and 6943 children in 2002). The results showed stable spread of bronchial asthma symptoms among the senior and junior pupils and prevalence of mild asthma with rare exacerbations in both the groups. A progredient growth of severe asthma attack rate was noted in eight-form pupils. An increase in frequency of combined upper and lower airway pathologies was registered with reduction in skin atopy rate. A problem of underestimating of the pathology was discussed.ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π° 6-Π»Π΅ΡΠ½ΡΡ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ° ΡΠΏΠΈΠ΄Π΅ΠΌΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΡ ΠΏΠΎ Π΅Π΄ΠΈΠ½ΡΠΌ ΡΡΠ°Π½Π΄Π°ΡΡΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΠΌ ΠΏΡΠΎΡΠΎΠΊΠΎΠ»Π°ΠΌ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ "ΠΠ΅ΠΆΠ΄ΡΠ½Π°ΡΠΎΠ΄Π½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π°ΡΡΠΌΡ ΠΈ Π°Π»Π»Π΅ΡΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ Ρ Π΄Π΅ΡΠ΅ΠΉ" (ISAAC) Π² ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ΅. ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΠΈ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡ ΠΏΡΠΈ ΡΠΏΠ»ΠΎΡΠ½ΠΎΠΌ Π°Π½ΠΊΠ΅ΡΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΡΠ΅Π½ΠΈΠΊΠΎΠ² 1-Ρ
ΠΈ 8-Ρ
ΠΊΠ»Π°ΡΡΠΎΠ² (7291 ΡΠΊΠΎΠ»ΡΠ½ΠΈΠΊ Π² 1996 Π³., 6631 β Π² 1999 Π³. ΠΈ 6943 β Π² 2002 Π³.). Π Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΠ΅ Π²ΡΡΠ²Π»Π΅Π½Ρ ΡΡΠ°Π±ΠΈΠ»ΡΠ½Π°Ρ ΡΠ°ΡΠΏΡΠΎΡΡΡΠ°Π½Π΅Π½Π½ΠΎΡΡΡ ΡΠΈΠΌΠΏΡΠΎΠΌΠΎΠ² Π±ΡΠΎΠ½Ρ
ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π°ΡΡΠΌΡ Ρ ΡΡΠ°ΡΡΠΈΡ
ΠΈ ΠΌΠ»Π°Π΄ΡΠΈΡ
ΡΠΊΠΎΠ»ΡΠ½ΠΈΠΊΠΎΠ², ΡΠΎΡ
ΡΠ°Π½Π΅Π½ΠΈΠ΅ ΠΏΡΠ΅ΠΎΠ±Π»Π°Π΄Π°Π½ΠΈΡ Π»Π΅Π³ΠΊΠΈΡ
ΡΠ΅Π΄ΠΊΠΎ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΈΡΠΎΠ²Π°Π²ΡΠΈΡ
ΡΠΎΡΠΌ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ Π² ΠΎΠ±Π΅ΠΈΡ
Π²ΠΎΠ·ΡΠ°ΡΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΠ°Ρ
. ΠΡΠΌΠ΅ΡΠ΅Π½ ΠΏΡΠΎΠ³ΡΠ΅Π΄ΠΈΠ΅Π½ΡΠ½ΡΠΉ ΡΠΎΡΡ ΡΡΠΆΠ΅Π»ΡΡ
ΠΏΡΠΈΡΡΡΠΏΠΎΠ² ΡΠ΄ΡΡΡΡ Ρ Π²ΠΎΡΡΠΌΠΈΠΊΠ»Π°ΡΡΠ½ΠΈΠΊΠΎΠ². Π ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΈ ΡΠΊΠΎΠ»ΡΠ½ΠΈΠΊΠΎΠ² Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΡΠΎΡΠ΅ΡΠ°Π½Π½ΠΎΠ³ΠΎ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ Π²Π΅ΡΡ
Π½ΠΈΡ
ΠΈ Π½ΠΈΠΆΠ½ΠΈΡ
ΠΎΡΠ΄Π΅Π»ΠΎΠ² ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° Ρ ΡΠΎΠΊΡΠ°ΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ°ΡΡΠΎΡΡ ΠΊΠΎΠΆΠ½ΡΡ
ΡΠΎΡΠΌ Π°ΡΠΎΠΏΠΈΠΈ. ΠΡΡΠ°ΠΆΠ΅Π½Π° ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ° Π³ΠΈΠΏΠΎΠ΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΠΈ
ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² ΠΏΡΠΈ Π²ΠΈΡΡΡΠ½ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ , ΠΏΡΠΎΡΠ΅ΠΊΠ°ΡΡΠΈΡ Ρ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ Ρ Π΄Π΅ΡΠ΅ΠΉ
The aim of the study was to study the characteristics of the content of certain cytokines in serum as indicators of the immune response in viral infections accompanied by diarrhea syndrome in children. Materials and research methods. A survey was conducted of 92 children aged 3 months to 15 years with acute respiratory viral infections hospitalized in hospital No. 6 in Novosibirsk in the first 2 days from the onset of the disease. Two groups were formed β group 1 β patients with diarrheal syndrome and signs of respiratory system damage. Group 2 β children with isolated lesions of the respiratory tract. In all children, the genetic material of viruses was determined by polymerase chain reaction (PCR) in the nose and throat washes. Also, in all children, the determination of serum levels of IL-8, IL-17, IFN-Γ£ by ELISA was performed. Results and discussion. When studying the levels of cytokines (IFN-Γ£, IL-8, IL-17) in patients with signs of gastrointestinal tract lesions in comparison with isolated respiratory manifestations, no statistically significant differences were found. However, when analyzing the levels of the studied parameters in patients with diarrheal syndrome in different age groups, significant differences in the levels of IFN-Γ£ and IL-8 were revealed.Π¦Π΅Π»ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²ΠΈΠ»ΠΎΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΊΠ°ΠΊ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ ΡΠ΅Π°Π³ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΈ Π²ΠΈΡΡΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ
, ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°ΡΡΠΈΡ
ΡΡ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ Ρ Π΄Π΅ΡΠ΅ΠΉ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ 92 Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 3 ΠΌΠ΅ΡΡΡΠ΅Π² Π΄ΠΎ 15 Π»Π΅Ρ Ρ ΠΎΡΡΡΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ Π²ΠΈΡΡΡΠ½ΡΠΌΠΈ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΠΌΠΈ, Π³ΠΎΡΠΏΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π² ΡΡΠ°ΡΠΈΠΎΠ½Π°Ρ ΠΠΠ β6 Π³ΠΎΡΠΎΠ΄Π° ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ° Π² ΠΏΠ΅ΡΠ²ΡΠ΅ Π΄Π²ΠΎΠ΅ ΡΡΡΠΎΠΊ ΠΎΡ Π½Π°ΡΠ°Π»Π° Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ. Π‘ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Ρ Π΄Π²Π΅ Π³ΡΡΠΏΠΏΡ: I Π³ΡΡΠΏΠΏΠ° β ΠΏΠ°ΡΠΈΠ΅Π½ΡΡ Ρ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ ΠΈ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌΠΈ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ. ΠΡΡΠΏΠΏΠ° II β Π΄Π΅ΡΠΈ Ρ ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ°. Π£ Π²ΡΠ΅Ρ
Π΄Π΅ΡΠ΅ΠΉ ΠΏΡΠΎΠ²Π΅Π»ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π²ΠΈΡΡΡΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ (ΠΠ¦Π ) Π² ΡΠΌΡΠ²Π°Ρ
ΠΈΠ· Π½ΠΎΡΠΎ- ΠΈ ΡΠΎΡΠΎΠ³Π»ΠΎΡΠΊΠΈ. Π’Π°ΠΊΠΆΠ΅ Ρ Π²ΡΠ΅Ρ
Π΄Π΅ΡΠ΅ΠΉ Π±ΡΠ»ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ IL-8, IL-17, IFN-Γ£ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ€Π. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈ ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. ΠΡΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΡΡΠΎΠ²Π½Π΅ΠΉ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² (IFN-Γ£, IL-8, IL-17) Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌΠΈ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎ-ΠΊΠΈΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° Π² ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ Ρ ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡΠΌΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΡ
ΡΠ°Π·Π»ΠΈΡΠΈΠΉ Π²ΡΡΠ²Π»Π΅Π½ΠΎ Π½Π΅ Π±ΡΠ»ΠΎ. ΠΠ΄Π½Π°ΠΊΠΎ ΠΏΡΠΈ Π°Π½Π°Π»ΠΈΠ·Π΅ ΡΡΠΎΠ²Π½Π΅ΠΉ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π²ΠΎΠ·ΡΠ°ΡΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΠ°Ρ
Π²ΡΡΠ²Π»Π΅Π½Ρ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΡΠ΅ ΡΠ°Π·Π»ΠΈΡΠΈΡ Π² ΡΡΠΎΠ²Π½Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ IFN-Γ£ ΠΈ IL-8
Microfluidic droplet platform for ultrahigh-throughput single-cell screening of biodiversity
Β© 2017, National Academy of Sciences. All rights reserved.Ultrahigh-throughput screening (uHTS) techniques can identify unique functionality from millions of variants. To mimic the natural selection mechanisms that occur by compartmentalization in vivo, we developed a technique based on single-cell encapsulation in droplets of a monodisperse microfluidic double water-in-oil-in-water emulsion (MDE). Biocompatible MDE enables in-droplet cultivation of different living species. The combination of droplet-generating machinery with FACS followed by next-generation sequencing and liquid chromatography-mass spectrometry analysis of the secretomes of encapsulated organisms yielded detailed genotype/phenotype descriptions. This platform was probed with uHTS for biocatalysts anchored to yeast with enrichment close to the theoretically calculated limit and cell-to-cell interactions. MDE-FACS allowed the identification of human butyrylcholinesterase mutants that undergo self-reactivation after inhibition by the organophosphorus agent paraoxon. The versatility of the platform allowed the identification of bacteria, including slow-growing oral microbiota species that suppress the growth of a common pathogen, Staphylococcus aureus, and predicted which genera were associated with inhibitory activity
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