Synthesis and Cytotoxicity Screening of Some Novel Benzofuranoyl-pyrazole Derivatives against Liver and Cervix Carcinoma Cell Lines

A series of new pyrazole, thiazole and thiazolinone derivatives incorporated into benzofuran were synthesized by using 3-(benzofuran-2-yl)-1-phenyl-1H-pyrazol-4-carboxaldehyde as starting material. A total of 41 novel compounds were synthesized. Some of these synthesized compounds were evaluated for cytotoxicity activity against HEPG2 (liver carcinoma cell line) and HELA (cervix carcinoma cell line). The tested compounds (1, 2c, 7c, 8b, 9b, 13b and 14b) showed better activities at low concentration against the commonly used human carcinoma cell lines. A detailed synthesis, spectroscopic data and cytotoxicity screening for the new compounds are described.Keywords: Pyrazole, thiazolinone, benzofuran, benzofuranoyl-pyrazoles, cytotoxic screening, HEPG2, HELAPDF and Supplementry file attache

Control of saltwater intrusion by aquifer storage and recovery

This is the final version of the article. Available from the publisher via the DOI in this record.Published Online 18th August 2016This paper presents the results obtained from the application of aquifer storage and recovery (ASR) technique to control seawater intrusion (SWI) in coastal aquifers. The study is based on the numerical modelling experiments performed using the SUTRA (Saturated–Unsaturated TRAnsport) finite-element code on the Wadi Ham aquifer in the UAE. A three-dimensional numerical model of this aquifer is developed and calibrated based on the available hydrogeological data in real scale. A significant amount of SWI has been calculated for the year 2015 due to the high rates of pumping from the available local well fields. To study the future responses of the aquifer to different control actions, the transient responses of SWI are simulated over a 10-year planning horizon. The proposed management measure (ASR) is implemented in repeated cycles of artificial recharge, storage and recovery using an additional set of wells defined in the model. The results show that ASR is a reliable method in controlling SWI in coastal aquifer systems besides its conventional role in subsurface water banking.The authors acknowledge their gratitude to the British Council, UK, for providing funding (project code: SH-04509) to support this research

Virus free seed potato production through sprout cutting technique under net-house

In order to evaluate the performance of sprout cutting for seed potato production against virus infection, a study was conducted at Bangladesh Agricultural Development Corporation (BADC) Foundation Seed Potato Production Farm, Domar, Nilphamary, Bangladesh in 2005 - 2006. Sprout cut seedlings were grown under three production practices viz. Net-house practice, BADC practice and farmers’ practice. Sprout cutting technique was proved to be an effective method of seed potato production against potato virus Y (PVY) and potato leaf roll virus (PLRV). The incidence of PVY and PLRV was detected as nil in net-house practice. However, PVY and PLRV were prevalent in BADC practice and farmers’ practice although, BADC practice performed better over farmers’ practice. Yield of seed potato and non-seed tubers along with yield attributes were found to be higher in all the five varieties (Diamant, Baraka, Asterix, Raja and Provento) in net-house practice followed by BADC practice and farmers’ practice. Among the varieties Diamant, Asterix and Raja seemed to be better in performance as compared to the others. In most of the cases net-house practice differed significantly (p = 0.05) with the others.Keywords: Potato virus Y, potato leaf roll virus, seed potato, sprout cuttingAfrican Journal of Biotechnology Vol. 9(36), pp. 5852-5858, 6 September, 201

Investigating usability of mobile health applications in Bangladesh

Background Lack of usability can be a major barrier for the rapid adoption of mobile services. Therefore, the purpose of this paper is to investigate the usability of Mobile Health applications in Bangladesh. Method We followed a 3-stage approach in our research. First, we conducted a keyword-based application search in the popular app stores. We followed the affinity diagram approach and clustered the found applications into nine groups. Second, we randomly selected four apps from each group (36 apps in total) and conducted a heuristic evaluation. Finally, we selected the highest downloaded app from each group and conducted user studies with 30 participants. Results We found 61% usability problems are catastrophe or major in nature from heuristic inspection. The most (21%) violated heuristic is aesthetic and minimalist design. The user studies revealed low System Usability Scale (SUS) scores for those apps that had a high number of usability problems based on the heuristic evaluation. Thus, the results of heuristic evaluation and user studies complement each other. Conclusion Overall, the findings suggest that the usability of the mobile health apps in Bangladesh is not satisfactory in general and could be a potential barrier for wider adoption of mobile health services

Probing ultrafast carrier dynamics and nonlinear absorption and refraction in core-shell silicon nanowires

We investigate the relaxation dynamics of photogenerated carriers in silicon nanowires consisting of a crystalline core and a surrounding amorphous shell, using femtosecond time-resolved differential reflectivity and transmission spectroscopy at photon energies of 3.15 eV and 1.57 eV. The complex behavior of the differential transmission and reflectivity transients is the mixed contributions from the crystalline core and the amorphous silicon on the nanowire surface and the substrate where competing effects of state filling and photoinduced absorption govern the carrier dynamics. Faster relaxation rates are observed on increasing the photo-generated carrier density. Independent experimental results on crystalline silicon-on-sapphire help us in separating the contributions from the carrier dynamics in crystalline core and the amorphous regions in the nanowire samples. Further, single beam z-scan nonlinear transmission experiments at 1.57 eV in both open and close aperture configurations yield two-photon absorption coefficient \$beta$ (~3 cm/GW) and nonlinear refraction coefficient \$gamma$ (-2.5x10^-4 cm2/GW).Comment: 6 pages, 6 figure

Screening of barley genotypes for drought tolerance based on culm reserves contribution to grain yield

Grain filling determines the grain weight, a major component of grain yield in cereals. Grain filling in barley depends on current assimilation and culm reserves. A pot experiment was conducted at the Grilled House, Department of Crop Botany, Bangladesh Agricultural University, Mymensingh during October 2015–May 2016 to study the grain filling patterns and the contributions of culm reserves to grain yield under drought stress. The experiment consisted of two factors—barley cultivars (six cultivars) and drought stress treatments (control and drought stress). Drought stress was imposed by limiting the irrigation during grain filling period. The tillers were sampled at anthesis, milk-ripe and maturity to determine the changes in dry weights of different parts, viz., leaf lamina, culm with sheath, spikes, and grains; and to examine the contribution of culm reserves to grain yield. The result in this experiment revealed that the grain yield was reduced by 5–25% due to drought stress. The reduction in grain yield was attributable to reduce number of grains per spike and lighter grain weight due to the stress. Drought stress drastically reduced the grain filling duration by about 30% and the stress induced early leaf senescence. Photosynthesis rate and leaf greenness were also reduced in stress. The stress altered the contribution of culm reserves, water soluble carbohydrates (WSCs) in culms to grains. At milk ripe stage, accumulation reached its peak. It accumulated 29.0 to 70.0 mg and from 15.8 to 40.6 mg culm−1 in control and stressed plants, respectively. The residual culm WSCs ranged from 3.5 to 11.2 mg and 1.0 to 3.5 mg culm−1 under control and stress conditions, respectively. The highest contribution of culm WSCs to grain yield was observed in BARI barley2 and the lowest was in BARI barley5 both in control and stress condition. Among the cultivars studied, BARI barley2 produced higher yield with the higher contribution of culm reserves to grain yield under the drought stress

Computation of non-isothermal thermo-convective micropolar fluid dynamics in a Hall MHD generator system with non-linear distending wall

A theoretical model for steady non-isothermal convective heat transfer in non-Newtonian magnetized micropolar gas flow from a non-linear stretching/contracting wall in the presence of strong magnetic field is presented, as a simulation of an MHD (magnetohydrodynamic) Hall energy generator. Subsonic flow is considered, and compressibility effects neglected. The strength of the magnetic field which is applied in the general case obliquely to the wall is sufficient to invoke the collective effects of Hall current and Ohmic heating (Joule dissipation). Viscous heating is also included in the energy balance. Deploying similarity transformations, the governing equations are normalized into nonlinear ordinary differential equations with associated boundary conditions. The non-linear boundary value problem thus posed is then solved computationally with Nachtsheim-Swigert iteration technique along with the fourth-fifth order Runge-Kutta integration method (RKM). Verification of solutions is obtained with the semi-analytical Homotopy analysis method (HAM). Further validation is conducted with the semi-numerical Adomian Decomposition Method (ADM). In both cases excellent agreement is obtained with the Runge-Kutta shooting quadrature solutions. Additional validation is conducted with earlier Newtonian studies in the absence of micropolar, Hall current and dissipation effects. The influence of local Grashof number, local Hartmann number, Eringen microrotational parameter, Eringen coupling vortex parameter, Prandtl number and Eckert number on non-dimensional velocity components (primary, secondary and angular) and temperature within the boundary layer are graphically illustrated and interpreted at length. Furthermore, the effects of the thermophysical (e.g. non-isothermal power law index), electromagnetic parameters (e.g. Hall parameter) and geometric parameter (wall extension/contraction parameter) on the skinfriction coefficient (i.e. primary and secondary shear stress and wall couple stress) and surface heat transfer rate (Nusselt number) are evaluated. The study is relevant to near wall transport phenomena in novel MHD Hall power generators

Evaluation of adjunctive HPV testing by Hybrid Capture II(® )in women with minor cytological abnormalities for the diagnosis of CIN2/3 and cost comparison with colposcopy

BACKGROUND: As a proportion of high grade cervical intraepithelial neoplasia (CIN2/3) are associated with equivocal cervical smears, which show borderline or mild dyskaryosis, follow up with repeat smears, colposcopy and biopsy is required. Since infection with oncogenic Human Papilloma Virus (HR HPV) has been found to be associated with the development of cervical cancer, HRHPV testing appears to be an alternative. OBJECTIVE: The present study assesses if HRHPV testing can predict CIN2/3 in women referred for mild dyskaryosis and borderline cytological changes in an health authority with a referral policy to colposcopy after one single mild dyskaryotic Pap smear. STUDY DESIGN: The HPV DNA Hybrid Capture II (Digene/Abbott, Maidenhead) was evaluated on 110 consenting women with mild dyskaryosis and 23 women with persistent borderline changes, who were referred for colposcopy between May and November 2001. A cost comparison between two referral policies was performed. RESULTS: CIN2/3 was diagnosed histologically in 30 of 133 women (22%) with minor cytological abnormalities. As the Receiver Operator Characteristics plot suggested a cut-off of 3 pg/ml the HRHPV HCII was evaluated at 3 RLU (relative light units) and at the manufacturer's recommendation of 1 RLU. At both cut-offs sensitivity and negative predictive value were high at 97%. Specificity was low at 37% at a cut-off of 1 pg/ml and 46% at a cut-off of 3 RLU. To remain cost neutral in comparison to immediate colposcopy the costs for one HR HPV HC II must not exceed £34.37 per test at a cut off of 3 pg/ml. CONCLUSION: The negative likelihood ratio (NLR) was of good diagnostic value with 0.089 at 1 RLU and 0.072 at 3 RLU, which reduces the post-test probability for CIN2/3 to 2% in this population. Women with minor cytological disorders can be excluded from colposcopy on a negative HR HPV result. Specificity can be improved by restricting HR HPV testing to women with persistent borderline cytological changes or to women over 30 years

Early rheumatoid arthritis is characterized by a distinct and transient synovial fluid cytokine profile of T cell and stromal cell origin

Pathological processes involved in the initiation of rheumatoid synovitis remain unclear. We undertook the present study to identify immune and stromal processes that are present soon after the clinical onset of rheumatoid arthritis ( RA) by assessing a panel of T cell, macrophage, and stromal cell related cytokines and chemokines in the synovial fluid of patients with early synovitis. Synovial fluid was aspirated from inflamed joints of patients with inflammatory arthritis of duration 3 months or less, whose outcomes were subsequently determined by follow up. For comparison, synovial fluid was aspirated from patients with acute crystal arthritis, established RA and osteoarthritis. Rheumatoid factor activity was blocked in the synovial fluid samples, and a panel of 23 cytokines and chemokines measured using a multiplex based system. Patients with early inflammatory arthritis who subsequently developed RA had a distinct but transient synovial fluid cytokine profile. The levels of a range of T cell, macrophage and stromal cell related cytokines ( e. g. IL-2, IL-4, IL-13, IL-17, IL-15, basic fibroblast growth factor and epidermal growth factor) were significantly elevated in these patients within 3 months after symptom onset, as compared with early arthritis patients who did not develop RA. In addition, this profile was no longer present in established RA. In contrast, patients with non-rheumatoid persistent synovitis exhibited elevated levels of interferon-gamma at initiation. Early synovitis destined to develop into RA is thus characterized by a distinct and transient synovial fluid cytokine profile. The cytokines present in the early rheumatoid lesion suggest that this response is likely to influence the microenvironment required for persistent RA

Ontology-driven, adaptive, medical questionnaires for patients with mild learning disabilities

Patients with Learning Disabilities (LD) have substantial and unmet healthcare needs, and previous studies have highlighted that they face both health inequalities and worse outcomes than the general population. Primary care practitioners are often the first port-of-call for medical consultations, and one issue faced by LD patients in this context is the very limited time available during consultations - typically less than ten minutes. In order to alleviate this issue, we propose a digital communication aid in the form of an ontology-based medical questionnaire that can adapt to a patient’s medical context as well as their accessibility needs (physical and cognitive). The application is intended to be used in advance of a consultation so that a primary care practitioner may have prior access to their LD patients’ self-reported symptoms. This work builds upon and extends previous research carried out in the development of adaptive medical questionnaires to include interactive and interface functionalities designed specifically to cater for patients with potentially complex accessibility needs. A patient’s current health status and accessibility profile (relating to their impairments) is used to dynamically adjust the structure and content of the medical questionnaire. As such, the system is able to significantly limit and focus questions to immediately relevant concerns while discarding irrelevant questions. We propose that our ontology-based design not only improves the relevance and accessibility of medical questionnaires for patients with LDs, but also provides important benefits in terms of medical knowledge-base modularity, as well as for software extension and maintenance