Diversity of agroecosystems and ecosystem services gain for agrobiodiversity conservation in agricultural landscape in northern Togo

To tackle food insecurity and alleviate rural poverty, the Togolese government initiated a novel form of land-based investment in rural agriculture named «Kara agropole», which promotes the cultivation of maize, rice, sesame, soybean and cashew mainly for commercial and industrial purposes. This study analyses the agricultural diversity as a baseline to foster the sustainable management of agrobiodiversity while implementing the project. The study was carried out in 11 counties from 4 districts (Bassar, Dankpen, Doufelgou and Kéran) in Kara region. A survey of 115 households was conducted to collect data about agricultural practices. Botanical inventory had been carried out in 60 square plots of 25mx25m to evaluate the current agrobiodiversity and to identify ecosystem services. Data analysis matching with empirical field observations showed that there are 6 types of agroecosystems (pure cropping – crop rotation, intercropping, agroforestry, forestry, fallow lands and pasture lands) in the area with pure cropping as the main agricultural practice. Provisioning services turn out to be of the highest interest to the survey respondents for annual and perennial species. Therefore, a landscape approach that strikes a balance between agricultural land use and conservation might help to conserve more  agrobiodiversity and promote other categories of ecosystem services. Keywords: Agroecosystems, ecosystem services, species, sustainable agriculture, Togo. French title: Diversite des agroecosystemes et benefice des services ecosystemiques pour la conservation de l’agrobiodiversite en paysage agricole au nord TogoPour lutter contre l’insécurité alimentaire et la pauvreté rurale, le gouvernement togolais a lancé une nouvelle forme d’investissement foncier en agriculture rurale, appelée « agropole de Kara », favorisant la culture du maïs, du riz, du sésame, du soja et de l’anacarde aux fins commerciales et industrielles. La présente étude analyse la diversité agricole comme référence pour favoriser la gestion durable del’agrobiodiversité pendant la mise en œuvre du projet. L’étude est réalisée dans 11 cantons de 4 préfectures (Bassar, Dankpen,  Doufelgou et Kéran) de la région de Kara. Une enquête menée auprès de 115 ménages a recueilli des données sur les pratiques  agricoles. L’inventaire botanique est réalisé dans 60 parcelles carrées de 25mx25m pour évaluer l’agrobiodiversité présente et identifier les services écosystémiques. L’analyse de données couplées à l’observation empirique montrent l’existence de 6 types d’agroécosystèmes (culture pure, cultures associées, agroforesterie, sylviculture, jachère et pâturage) dans la zone, avec la culture pure comme principale pratique agricole. Les services d’approvisionnement sont d’un grand intérêt aux répondants enquêtés pour les espèces annuelles et pérennes. Par conséquent, l’approche paysage qui établit un équilibre entre utilisation et conservation des terres agricoles pourrait contribuer à conserver davantage l’agrobiodiversité et promouvoir d’autres catégories de services écosystémiques. Mots-clés : Agroécosystèmes, services écosystémiques, espèces, agriculture durable, Tog

Knowledge, Perception of Risk and Attitude of Sierra Leone Military Personnel towards Colleagues with HIV/AIDS

The aim of this survey was to gain an insight into the level of knowledge, perception of risk and attitude of Sierra Leone Military personnel towards colleagues with HIV/AIDS. Four hundred and fifty (450) randomly selected male and female military personnel including officers and other ranks from six battalions were surveyed with a standard questionnaire. Results of this survey demonstrated a relatively low level of knowledge of HIV and AIDS amongst the survey population as evidenced by the 40% and 52% of participants stating that HIV is transmitted by mosquito bites and from public toilets respectively. An equally low perception of risk of the infection was demonstrated from the responses of the participants about attitude towards HIV infected colleagues. Three-fourth of the participants indicated that nobody should be informed if a colleague is HIV positive, with almost all the participants expressing their willingness to take care of an HIV/AIDS person in their household. Varying responses obtained demonstrate the necessity for scaling-up HIV education within the Republic of Sierra Leone Armed Forces. More resources most therefore be made available to the HIV/AIDS office of the Republic of Sierra Leone Armed Forces so that HIV education activities can be extended to all the brigades and battalions. Keywords: Knowledge, Perception, Attitude, HIV/AIDS

Knowledge, beliefs and attitude towards malaria control and prevention among students in tertiary institutions in the Gambia

BACKGROUNDEven though Malaria caused by five parasite species, two of which – Plasmodium falciparum and Plasmodium vivax is preventable, curable and treatable, it continues to pose a significant health threat to many communities around the world. Particularly in Sub-Saharan Africa, The Gambia is one of the African countries that has seen a significant reduction in malaria cases. Malaria cases in The Gambia had decreased gradually from 346.9 per 100,000 persons in 2004 to 66 per 100,000 people in 2018. The fight against malaria is great progress for the future. AIMThis study aimed at assessing the knowledge, attitude and practice of students attending tertiary institutions in The Gambia in regard to Malaria prevention and control. METHODOLOGYFrom May to June 2021, a standardized pre-tested semi-structured questionnaire was used to obtain information from 431 students in four public institutions in The Gambia. The University of The Gambia (UTG) Brikama Campus, The Gambia College-Brikama Campus, Management Development Institution (MDI) and The Gambia Technical Training Institution (GTTI. Respondents were chosen using a random sampling approach of students who were found on campuses and consented to participate. Sample size was determined using the formula described by Thrusfield (2007) based on a 95% confidence interval. It was first entered into excel and then exported to SPSS version 22 (SPSS Inc., Chicago, Ill., USA) for data analyses. RESULTThe aetiology, symptoms and therapy of malaria were all well-understood by the respondents. Age (P-value=0.005) and program of study (P-value=0.014) were highly significant with students’ knowledge on the mode of transmission of malaria as for students belief of the disease. Institution of learning Odd ratio (1.385, P value=0.003) was the only factor which affected students perception of malaria. Gender (Odd ratio=2.491, P-value=0.005) and the institution of learning (Odd ratio=1.506, P-value=0.003) were factors which had a high statistical significance with students practice of sleep under an ITNs. CONCLUSIONThis study reported a high level of knowledge, poor attitude and practice towards malaria control interventions among students attending tertiary institutions in The Gambia. Students from the UTG and GTTI showed a better positive knowledge than those from the other participants. Their curriculum studies and social activities (individual students’ unions) exposed them to a higher level of awareness. RECOMMENDATIONSHealth-related programs on malaria prevention and control should be organized to raise awareness in through television and radio or any other media. Health education should be a compulsory topic or module in institutions

Demographics and survival of AIDS cases with cancer, Washington, DC, 1996-2006

Background Washington, DC (DC) has one of the highest HIV/AIDS rates in the U.S and cancer is the second leading cause of death among DC residents. This study sought to examine the demographic characteristics and survival of persons with AIDS defining cancers (ADCs) compared to those with non-AIDS defining cancers (NADCs) between the early HAART era (1996-2001) and the late HAART era (2002-2006) in DC. Methods Cases reported from 1996-2006 to the DC Cancer Registry and the AIDS Surveillance Registry were linked using a probabilistic matching algorithm. Cases were included if the cancer occurred from 4 months to 60 months post-AIDS diagnosis and were stratified into ADCs and NADCs for analyses. Cancer diagnoses were stratified into the early and late HAART eras to compare the availability of HAART on the distribution of cancer type. Kaplan-Meier survival analysis and adjusted Cox proportional hazards regression were used to assess survival time and risk of death by cancer type. Results From 1996-2006, among 8,800 AIDS cases, 300 (3.4%) cases had a cancer diagnosis. NADCs accounted for 51% of cancers and were significantly more likely to be diagnosed with AIDS (p\u3c0.0001) and cancer (p\u3c0.0001) at 40 years or older and had a significantly longer median time from AIDS to cancer diagnosis (2.46 vs. 1.75 years, p=0.01) compared to ADCs. The most common ADCs were Kaposi sarcoma (40%) and non-Hodgkin lymphoma (NHL) (44%); the most common NADC cases were lung (20%), Hodgkin lymphoma (8%) and anal (8%) cancer. ADCs accounted for 56% of cancer cases in the late-HAART as compared to the early-HAART period (45%). Mortality within the first year of cancer diagnosis was similar (ADC 41% vs. NADC 37%) and no statistical difference in survival time was observed. In the adjusted model, NHL and lung cases were significantly more likely to die as compared to other cancers (NHL HR=3.06; Lung HR=3.44). Conclusions In DC, despite high HIV/AIDS and cancer prevalence, only a small proportion of AIDS cases also develop cancer with ADCs and NADCs being equally common. HAART availability does not seem to have altered survival among ADCs and NADCs. Survival among NHL cases was relatively low reflecting the need for increased access to care among HIV+ persons. NADC cases are most likely developing cancers related to advancing age with higher proportions of lung cancers being observed. Public health efforts should focus on lung cancer prevention and continued monitoring of HIV-infected persons for cancers

Ebola viral load at diagnosis associates with patient outcome and outbreak evolution

BACKGROUND. Ebola virus (EBOV) causes periodic outbreaks of life-threatening EBOV disease in Africa. Historically, these outbreaks have been relatively small and geographically contained; however, the magnitude of the EBOV outbreak that began in 2014 in West Africa has been unprecedented. The aim of this study was to describe the viral kinetics of EBOV during this outbreak and identify factors that contribute to outbreak progression. METHODS. From July to December 2014, one laboratory in Sierra Leone processed over 2,700 patient samples for EBOV detection by quantitative PCR (qPCR). Viremia was measured following patient admission. Age, sex, and approximate time of symptom onset were also recorded for each patient. The data was analyzed using various mathematical models to find trends of potential interest. RESULTS. The analysis revealed a significant difference (P = 2.7 × 10–77) between the initial viremia of survivors (4.02 log10 genome equivalents [GEQ]/ml) and nonsurvivors (6.18 log10 GEQ/ml). At the population level, patient viral loads were higher on average in July than in November, even when accounting for outcome and time since onset of symptoms. This decrease in viral loads temporally correlated with an increase in circulating EBOV-specific IgG antibodies among individuals who were suspected of being infected but shown to be negative for the virus by PCR. CONCLUSIONS. Our results indicate that initial viremia is associated with outcome of the individual and outbreak duration; therefore, care must be taken in planning clinical trials and interventions. Additional research in virus adaptation and the impacts of host factors on EBOV transmission and pathogenesis is needed

Isolation of Angola-like Marburg virus from Egyptian rousette bats from West Africa.

Marburg virus (MARV) causes sporadic outbreaks of severe Marburg virus disease (MVD). Most MVD outbreaks originated in East Africa and field studies in East Africa, South Africa, Zambia, and Gabon identified the Egyptian rousette bat (ERB; Rousettus aegyptiacus) as a natural reservoir. However, the largest recorded MVD outbreak with the highest case-fatality ratio happened in 2005 in Angola, where direct spillover from bats was not  shown. Here, collaborative studies by the Centers for Disease Control and Prevention, Njala University, University of California, Davis USAID-PREDICT, and the University of Makeni identify MARV circulating in ERBs in Sierra Leone. PCR, antibody and virus isolation data from 1755 bats of 42 species shows active MARV infection in approximately 2.5% of ERBs. Phylogenetic analysis identifies MARVs that are similar to the Angola strain. These results provide evidence of MARV circulation in West Africa and demonstrate the value of pathogen surveillance to identify previously undetected threats

The Sleep Quality- and Myopia-Linked PDE11A-Y727C Variant Impacts Neural Physiology by Reducing Catalytic Activity and Altering Subcellular Compartmentalization of the Enzyme

Recently, a Y727C variant in the dual-specific 3′,5′-cyclic nucleotide phosphodiesterase 11A (PDE11A-Y727C) was linked to increased sleep quality and reduced myopia risk in humans. Given the well-established role that the PDE11 substrates cAMP and cGMP play in eye physiology and sleep, we determined if (1) PDE11A protein is expressed in the retina or other eye segments in mice, (2) PDE11A-Y7272C affects catalytic activity and/or subcellular compartmentalization more so than the nearby suicide-associated PDE11A-M878V variant, and (3) Pde11a deletion alters eye growth or sleep quality in male and female mice. Western blots show distinct protein expression of PDE11A4, but not PDE11A1-3, in eyes of Pde11a WT, but not KO mice, that vary by eye segment and age. In HT22 and COS-1 cells, PDE11A4-Y727C reduces PDE11A4 catalytic activity far more than PDE11A4-M878V, with both variants reducing PDE11A4-cAMP more so than PDE11A4-cGMP activity. Despite this, Pde11a deletion does not alter age-related changes in retinal or lens thickness or axial length, nor vitreous or anterior chamber depth. Further, Pde11a deletion only minimally changes refractive error and sleep quality. That said, both variants also dramatically alter the subcellular compartmentalization of human and mouse PDE11A4, an effect occurring independently of dephosphorylating PDE11A4-S117/S124 or phosphorylating PDE11A4-S162. Rather, re-compartmentalization of PDE11A4-Y727C is due to the loss of the tyrosine changing how PDE11A4 is packaged/repackaged via the trans-Golgi network. Therefore, the protective impact of the Y727C variant may reflect a gain-of-function (e.g., PDE11A4 displacing another PDE) that warrants further investigation in the context of reversing/preventing sleep disturbances or myopia.</p